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AB246528

Mouse IGFBP3 ELISA Kit

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Mouse IGFBP3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse IGFBP3 with a sensitivity of 41.08 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Igfbp-3, Igfbp3, Insulin-like growth factor-binding protein 3, IBP-3, IGF-binding protein 3, IGFBP-3

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Sandwich ELISA - Mouse IGFBP3 ELISA Kit (AB246528)
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Supplier Data

Sandwich ELISA - Mouse IGFBP3 ELISA Kit (AB246528)

Interpolated concentrations of native IGFBP3 in mouse liver tissue culture supernatant samples.

The concentrations of IGFBP3 were measured in duplicates, interpolated from the IGFBP3 standard curves and corrected for sample dilution. Undiluted samples are as follows : liver supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP3 concentration was determined to be 7,305 pg/mL in liver supernatant.

Sandwich ELISA - Mouse IGFBP3 ELISA Kit (AB246528)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IGFBP3 ELISA Kit (AB246528)

Mouse IGFBP3 Standard Curve Comparison.

Standard Curve comparison between mouse IGFBP3 SimpleStep ELISA kit and traditional ELISA kit from leading competitor.

Sandwich ELISA - Mouse IGFBP3 ELISA Kit (AB246528)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IGFBP3 ELISA Kit (AB246528)

Example of mouse IGFBP3 standard curve in Sample Diluent NS.

The IGFBP3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse IGFBP3 ELISA Kit (AB246528)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IGFBP3 ELISA Kit (AB246528)

Interpolated concentrations of native IGFBP3 in mouse serum and plasma samples.

The concentrations of IGFBP3 were measured in duplicates, interpolated from the IGFBP3 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 500, plasma (citrate) 1 : 500, plasma (heparin) 1 : 500, and plasma (EDTA) 1 : 500. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP3 concentration was determined to be 1,461 ng/mL in serum, 1,546 ng/mL in plasma (citrate), 2,319 ng/mL in plasma (heparin), and 2,007 ng/ml in plasma (EDTA).

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Serum, Tissue Culture Media, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 41.08 pg/mL

Range

109.375 - 7000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse IGFBP3 ELISA Kit (ab246528) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IGFBP3 protein in cit plasma, edta plasma, hep plasma, serum, and tissue culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IGFBP3 with 41.08 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.3" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.2" } ]
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Recovery

[ { "sample": "Serum", "range": "99 - 109 %", "average": "= 104.5" }, { "sample": "Tissue Culture Media", "range": "88 - 104 %", "average": "= 101" }, { "sample": "EDTA Plasma", "range": "96 - 107 %", "average": "= 101" }, { "sample": "Heparin Plasma", "range": "102 - 110 %", "average": "= 107" }, { "sample": "Citrate plasma", "range": "90 - 102 %", "average": "= 96" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab246528-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB246528-CMP07", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB246528-CMP11", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB246528-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB246528-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse IGFBP3 Lyophilized Recombinant Protein", "number":"AB246528-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse IGFBP3 Capture Antibody", "number":"AB246528-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB246528-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse IGFBP3 Detector Antibody", "number":"AB246528-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB246528-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB246528-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R. Promotes testicular germ cell apoptosis.
See full target information Igfbp3
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websiteProtocolBooklet
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Product promise

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