JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB100693

Mouse IGFBP5 ELISA Kit

Be the first to review this product! Submit a review

|

(1 Publication)

Mouse IGFBP5 ELISA Kit is a Sandwich ELISA for the measurement of Mouse IGFBP5 in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Igfbp-5, Igfbp5, Insulin-like growth factor-binding protein 5, IBP-5, IGF-binding protein 5, IGFBP-5

1 Images
Sandwich ELISA - Mouse IGFBP5 ELISA Kit (AB100693)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IGFBP5 ELISA Kit (AB100693)

Representative Standard Curve using ab100693

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 5 pg/mL

Range

4.12 - 3000 pg/mL

Assay Platform

Microplate

style
left-column

Complementary Products

Primary Antibodies

AB242770

Anti-IGFBP5 antibody [EPR18013-137] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-IGFBP5 antibody [EPR18013-137] - BSA and Azide free (Detector) (AB242770)

  • 0
  • 0
Assay Kits

AB245726

Mouse Uromodulin ELISA Kit

Recombinant|SimpleStep

ELISA - Mouse Uromodulin ELISA Kit (AB245726)

  • 0
  • 0
Assay Kits

AB315043

Ms/Rt Synaptophysin ELISA Kit

SimpleStep

Sandwich ELISA - Ms/Rt Synaptophysin ELISA Kit (AB315043)

  • 0
  • 0
Assay Kits

AB302765

Human GRP78 BiP ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human GRP78 BiP ELISA Kit (AB302765)

  • 4
  • 1
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Abcam's IGFBP5 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IGFBP5 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for mouse IGFBP5 coated on a 96- well plate. Standards and samples are pipetted into the wells and IGFBP5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IGFBP5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGFBP5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Get better reproducibility in only 90 minutes with Mouse IGFBP5 ELISA Kit (ab208345) from our SimpleStep ELISA® range.

style
left-column

Recovery

[ { "sample": "Cell culture supernatant", "range": "80 - 101 %", "average": "= 91.26" }, { "sample": "Serum", "range": "86 - 130 %", "average": "= 115.3" }, { "sample": "Plasma", "range": "83 - 102 %", "average": "= 95.11" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100693-1x96Tests", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"Assay Diluent C", "number":"AB100693-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100693-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100693-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IGFBP5 Microplate (12 x 8 wells)", "number":"AB100693-CMP06", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100693-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100693-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse IGFBP5 Standard (lyophilized)", "number":"AB100693-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Mouse IGFBP5", "number":"AB100693-CMP09", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB100693-CMP02", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column
style
left-column

Product protocols

style
left-column

Target data

Multifunctional protein that plays a critical role in regulating the availability of IGFs to their receptors and thereby regulates IGF-mediated cellular processes including proliferation, differentiation, and apoptosis in a cell-type specific manner. Increases the cell proliferation of osteoblasts, intestinal smooth muscle cells and neuroblastoma cells (By similarity). Enhances adhesion and survival of epithelial cells but decreases adhesion of mesenchymal cells (PubMed : 24120850). Once secreted, acts as a major mediator of mTORC1-dependent feedback inhibition of IGF1 signaling (By similarity). Also plays a role in the induction of extracellular matrix (ECM) production and deposition independently of its nuclear translocation and binding to IGFs. Acts itself as a growth factor that can act independently of IGFs to regulate bone formation. Acts as a ligand for the ROR1 receptor which triggers formation of ROR1/HER2 heterodimer to enhance CREB oncogenic signaling (By similarity).
See full target information Igfbp5
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The international journal of biochemistry & cell b 45:2774-85 PubMed24120850

2013

IGFBP-5 enhances epithelial cell adhesion and protects epithelial cells from TGFβ1-induced mesenchymal invasion.

Applications

Unspecified application

Species

Unspecified reactive species

A Vijayan,D Guha,F Ameer,I Kaziri,C C Mooney,L Bennett,A Sureshbabu,E Tonner,J Beattie,G J Allan,J Edwards,D J Flint
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com