AB208345

Mouse IGFBP5 ELISA Kit

Recombinant
SimpleStep
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(1 Publication)

Mouse IGFBP5 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse IGFBP5 with a sensitivity of 73 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

3 Images

Sandwich ELISA - Mouse IGFBP5 ELISA Kit (AB208345)

sELISA

Supplier Data

Sandwich ELISA - Mouse IGFBP5 ELISA Kit (AB208345)

Example of mouse IGFBP5 standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse IGFBP5 ELISA Kit (AB208345)

Interpolated concentrations of native IGFBP5 in mouse serum and plasma samples.

The concentrations of IGFBP5 were measured in duplicates, interpolated from the IGFBP5 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 600, plasma 1 : 800, and plasma (heparin) 1 : 800. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP5 concentration was determined to be 2,058 ng/mL in serum, 3,670 ng/mL in plasma (citrate), and 3,865 ng/mL in plasma (heparin).

sELISA

Supplier Data

Sandwich ELISA - Mouse IGFBP5 ELISA Kit (AB208345)

This assay detects rat IGFBP5.

The concentrations of IGFBP5 in rat serum were measured in duplicates, interpolated from the mouse IGFBP5 standard curves and corrected for sample dilution. Undiluted samples are rat serum 1 : 250. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP5 concentration was determined to be 2691 ng/mL in rat serum.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 73 pg/mL

Range

125 - 8000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse IGFBP5 ELISA Kit (ab208345) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IGFBP5 protein in cell culture supernatant, serum, and plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IGFBP5 with 73 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Insulin-like growth factor-binding protein 5 (IGFBP5) is an IGF binding protein involved in prolonging its half-life, and has both inhibitory and stimulatory effects on IGFs. IGFBP5 can also alter the interaction of IGFs with their receptors.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Mouse Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.4" }, { "reproducibilityType": "Intra", "sample": "Mouse Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.6" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "102 - 106 %", "average": "= 104" }, { "sample": "Serum", "range": "83 - 96 %", "average": "= 88" }, { "sample": "Citrate plasma", "range": "92 - 102 %", "average": "= 98" }, { "sample": "Heparin Plasma", "range": "80 - 104 %", "average": "= 93" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Multifunctional protein that plays a critical role in regulating the availability of IGFs to their receptors and thereby regulates IGF-mediated cellular processes including proliferation, differentiation, and apoptosis in a cell-type specific manner. Increases the cell proliferation of osteoblasts, intestinal smooth muscle cells and neuroblastoma cells (By similarity). Enhances adhesion and survival of epithelial cells but decreases adhesion of mesenchymal cells (PubMed : 24120850). Once secreted, acts as a major mediator of mTORC1-dependent feedback inhibition of IGF1 signaling (By similarity). Also plays a role in the induction of extracellular matrix (ECM) production and deposition independently of its nuclear translocation and binding to IGFs. Acts itself as a growth factor that can act independently of IGFs to regulate bone formation. Acts as a ligand for the ROR1 receptor which triggers formation of ROR1/HER2 heterodimer to enhance CREB oncogenic signaling (By similarity).

See full target information Igfbp5

Alternative Names

Igfbp-5, Igfbp5, Insulin-like growth factor-binding protein 5, IBP-5, IGF-binding protein 5, IGFBP-5

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

American journal of physiology. Gastrointestinal and liver physiology 316:G15-G24 PubMed30406699

2018

Molecular characterization of a precision-cut rat liver slice model for the evaluation of antifibrotic compounds.

Applications

Unspecified application

Species

Unspecified reactive species

Xinqiang Huang,Hong Cai,Ron Ammar,Yan Zhang,Yihe Wang,Kandasamy Ravi,John Thompson,Gabor Jarai

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse IGFBP5 ELISA Kit

Sandwich ELISA - Mouse IGFBP5 ELISA Kit (AB208345)

Sandwich ELISA - Mouse IGFBP5 ELISA Kit (AB208345)

Sandwich ELISA - Mouse IGFBP5 ELISA Kit (AB208345)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Primary Antibodies

Assay Kits

Assay Kits

Assay Kits

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Alternative Names

Publications (1)

Product promise