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AB245712

Mouse IGFBP7 ELISA Kit

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(1 Publication)

Mouse IGFBP7 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse IGFBP7 with a sensitivity of 6.03 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Mac25, Igfbp7, Insulin-like growth factor-binding protein 7, IBP-7, IGF-binding protein 7, IGFBP-7, MAC25 protein

5 Images
ELISA - Mouse IGFBP7 ELISA Kit (AB245712)
  • ELISA

Supplier Data

ELISA - Mouse IGFBP7 ELISA Kit (AB245712)
Sandwich ELISA - Mouse IGFBP7 ELISA Kit (AB245712)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IGFBP7 ELISA Kit (AB245712)

Interpolated concentrations of native IGFBP7 in mouse lung extract (25 ug/mL load) and spleen extract (50 ug/mL load). The concentrations of IGFBP7 were measured in duplicate and interpolated from the IGFBP7 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP7 concentration was determined to be 2039.98 pg/mL in lung extract and 2422.48 pg/mL in spleen extract.

Sandwich ELISA - Mouse IGFBP7 ELISA Kit (AB245712)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IGFBP7 ELISA Kit (AB245712)

Example of mouse IGFBP7 standard curve in Sample Diluent NS. The IGFBP7 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse IGFBP7 ELISA Kit (AB245712)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IGFBP7 ELISA Kit (AB245712)

Interpolated concentrations of native IGFBP7 in mouse serum, plasma, urine, and cell culture supernatant samples. The concentrations of IGFBP7 were measured in duplicates, interpolated from the IGFBP7 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1%, plasma (citrate) 1%, plasma (EDTA) 1%, urine 1%, and 3T3-L1 cell culture supernatant 6.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP7 concentration was determined to be 87.42 ng/mL in serum, 74.99 ng/mL in plasma (citrate), 85.41 ng/mL in plasma (EDTA) 28.69 ng/mL in urine, and 24.46 ng/mL in 3T3-L1 cell culture supernatant.

Sandwich ELISA - Mouse IGFBP7 ELISA Kit (AB245712)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IGFBP7 ELISA Kit (AB245712)

Example of mouse IGFBP7 standard curve in 1X Cell Extraction Buffer PTR. The IGFBP7 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Urine, Tissue Extracts, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 6.03 pg/mL

Range

39.06 - 2500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse IGFBP7 ELISA Kit ab245712 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse IGFBP7 in cell culture supernatant, citrate plasma, EDTA plasma, serum, tissue extracts, urine. This SimpleStep sensitivity is 6.03 pg/mL.

How the assay works

Mouse IGFBP7 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse IGFBP7 ELISA Kit ab245712 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-IGFBP7 antibody [EPR21922-228] - BSA and Azide free (Capture) ab259554
- Anti-IGFBP7 antibody [EPR21922-278] - BSA and Azide free (Detector) ab259554

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "11.2" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.6" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "83 - 100 %", "average": "= 92" }, { "sample": "Serum", "range": "103 - 111 %", "average": "= 106" }, { "sample": "EDTA Plasma", "range": "92 - 104 %", "average": "= 97" }, { "sample": "Urine", "range": "100 - 104 %", "average": "= 102" }, { "sample": "Tissue Extracts", "range": "97 - 100 %", "average": "= 99" }, { "sample": "Citrate plasma", "range": "100 - 108 %", "average": "= 104" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab245712-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB245712-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB245712-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB245712-CMP09", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB245712-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB245712-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB245712-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse IGFBP7 Lyophilized Recombinant Protein", "number":"AB245712-CMP04", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB245712-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse IGFBP7 Capture Antibody", "number":"AB245712-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse IGFBP7 Detector Antibody", "number":"AB245712-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB245712-CMP06", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB245712-CMP10", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Binds IGF1 and IGF2 with a relatively low affinity. Stimulates prostacyclin (PGI2) production. Stimulates cell adhesion. Acts as a ligand for CD93 to play a role in angiogenesis.
See full target information Igfbp7
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature cardiovascular research 1:1195-1214 PubMed39196168

2022

Insulin-like growth factor-binding protein-7 (IGFBP7) links senescence to heart failure.

Applications

Unspecified application

Species

Unspecified reactive species

Liyong Zhang,David Smyth,Mohammad Al-Khalaf,Alice Blet,Qiujiang Du,Jordan Bernick,Michael Gong,Xu Chi,Yena Oh,Malaika Roba-Oshin,Elizabeth Coletta,Michel Feletou,Anthony O Gramolini,Kyoung-Han Kim,Thais Coutinho,James L Januzzi,Benoit Tyl,Andre Ziegler,Peter P Liu
View all publications
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websiteProtocolBooklet
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Product promise

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