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AB326893

Mouse IGFBP7 ELISA Kit, Chemiluminescent

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Mouse IGFBP7 ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Mouse IGFBP7 with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 11.43 - 25000 pg/ml with a sensitivity of 1.6 pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending

View Alternative Names

Mac25, Igfbp7, Insulin-like growth factor-binding protein 7, IBP-7, IGF-binding protein 7, IGFBP-7, MAC25 protein

3 Images
Sandwich ELISA - Mouse IGFBP7 ELISA Kit, Chemiluminescent (AB326893)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IGFBP7 ELISA Kit, Chemiluminescent (AB326893)

Interpolated concentrations of native IGFBP7 in mouse lung extract (25 ug/mL load) and spleen extract (50 ug/mL load). The concentrations of IGFBP7 were measured in duplicate and interpolated from the IGFBP7 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP7 concentration was determined to be 2039.98 pg/mL in lung extract and 2422.48 pg/mL in spleen extract.

Sandwich ELISA - Mouse IGFBP7 ELISA Kit, Chemiluminescent (AB326893)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IGFBP7 ELISA Kit, Chemiluminescent (AB326893)

Interpolated concentrations of native IGFBP7 in mouse serum, plasma, urine, and cell culture supernatant samples. The concentrations of IGFBP7 were measured in duplicates, interpolated from the IGFBP7 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1%, plasma (citrate) 1%, plasma (EDTA) 1%, urine 1%, and 3T3-L1 cell culture supernatant 6.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP7 concentration was determined to be 87.42 ng/mL in serum, 74.99 ng/mL in plasma (citrate), 85.41 ng/mL in plasma (EDTA) 28.69 ng/mL in urine, and 24.46 ng/mL in 3T3-L1 cell culture supernatant.

Sandwich ELISA - Mouse IGFBP7 ELISA Kit, Chemiluminescent (AB326893)
  • sELISA

Lab

Sandwich ELISA - Mouse IGFBP7 ELISA Kit, Chemiluminescent (AB326893)

Standard curve comparison between the Chemiluminescent Mouse IGFBP7 SimpleStep ELISA and colorimetric Mouse IGFBP7 SimpleStep ELISA (ab245712) in NS. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Luminescent

Sample types

Cell culture supernatant, Serum, EDTA Plasma, Urine, Tissue Extracts, Citrate plasma

Reacts with

Human, Mouse, Rat, Cow

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.6 pg/mL

Range

11.43 - 25000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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Product details

Mouse IGFBP7 ELISA Kit, Chemiluminescent (ab326893) is a rapid single-wash sandwich 55-min ELISA to measure Mouse IGFBP7 in Cell culture supernatant, Serum, EDTA Plasma, Urine, Tissue Extracts, Citrate plasma with a sensitivity of 1.6 pg/mL.

How the assay works

Mouse IGFBP7 ELISA Kit, Chemiluminescent (ab326893) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse IGFBP7 (ab326893) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-IGFBP7 antibody [EPR21922-228] - BSA and Aside free (Capture) ab259553
- Anti-IGFBP7 antibody [EPR21922-278] - BSA and Aside free (Detector) ab259554
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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.6" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 11.2" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "83 - 100 %", "average": "= 100" }, { "sample": "Serum", "range": "103 - 111 %", "average": "= 111" }, { "sample": "EDTA Plasma", "range": "92 - 104 %", "average": "= 104" }, { "sample": "Urine", "range": "100 - 104 %", "average": "= 104" }, { "sample": "Tissue Extracts", "range": "97 - 100 %", "average": "= 100" }, { "sample": "Citrate plasma", "range": "100 - 108 %", "average": "= 108" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Binds IGF1 and IGF2 with a relatively low affinity. Stimulates prostacyclin (PGI2) production. Stimulates cell adhesion. Acts as a ligand for CD93 to play a role in angiogenesis.
See full target information Igfbp7
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com