JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB273150

Mouse IgG ELISA kit - 30 minutes

Be the first to review this product! Submit a review

|

(0 Publication)

Mouse IgG ELISA kit - 30 minutes is a Sandwich (quantitative) ELISA for the measurement of Mouse IgG - 30 minutes in in Cell Culture Media, Biofluids samples.
1 Images
ELISA - Mouse IgG ELISA kit - 30 minutes (AB273150)
  • ELISA

Unknown

ELISA - Mouse IgG ELISA kit - 30 minutes (AB273150)

Mouse Immunoglobulin Quantification Standard curve.

Example of Mouse IgG quantitative kit Standard curve

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Ascites Fluid

Assay type

Sandwich (quantitative)

Sensitivity

= 6 ng/mL

Range

20 - 1900 ng/mL

Assay Platform

Microplate

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

The Mouse IgG quantification Kit (ab273150) provides a rapid and easy method (one step ELISA) for the quantitative determination of mouse IgG in cell culture supernatant and ascitic fluid.

The kit includes ready-to-use reagents necessary to analyze up to 90 samples in 30 min. Buffer solutions are color coded in order to simplify pipetting steps.

A polyclonal antibody specific to mouse IgG (H+L) is pre-coated onto microwells. Samples and standards are pipetted into microwells and mouse IgG present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-mouse IgG (H+L) antibody is pipetted and incubated simultaneously with samples. After washing microwells in order to remove any non specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of mouse IgG in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Sample H 1/1000", "replicates": 30, "mean": null, "standardDeviation": "4.97", "coefficientOfVariability": null }, { "reproducibilityType": "Inter", "sample": "Sample H 1/250", "replicates": 30, "mean": null, "standardDeviation": "3.45", "coefficientOfVariability": null }, { "reproducibilityType": "Inter", "sample": "Sample H 1/500", "replicates": 30, "mean": null, "standardDeviation": "2.99", "coefficientOfVariability": null }, { "reproducibilityType": "Intra", "sample": "G 1/100", "replicates": 9, "mean": "102.47 µg/mL", "standardDeviation": "10.38", "coefficientOfVariability": null }, { "reproducibilityType": "Intra", "sample": "Sample A 1/100", "replicates": 9, "mean": "10.63 µg/mL", "standardDeviation": "5.33", "coefficientOfVariability": null }, { "reproducibilityType": "Intra", "sample": "Sample B 1/100", "replicates": 9, "mean": "11.56 µg/mL", "standardDeviation": "5.33", "coefficientOfVariability": null }, { "reproducibilityType": "Intra", "sample": "Sample C 1/100", "replicates": 9, "mean": "22.61 pg/mL", "standardDeviation": "8.48", "coefficientOfVariability": null }, { "reproducibilityType": "Intra", "sample": "Sample D 1/100", "replicates": 9, "mean": "28.88 µg/mL", "standardDeviation": "10.03", "coefficientOfVariability": null }, { "reproducibilityType": "Intra", "sample": "Sample E 1/100", "replicates": 9, "mean": "66.82 µg/mL", "standardDeviation": "8.39", "coefficientOfVariability": null }, { "reproducibilityType": "Intra", "sample": "Sample F 1/100", "replicates": 9, "mean": "75.92 µg/mL", "standardDeviation": "9.9", "coefficientOfVariability": null } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab273150-1x96Tests", "assetComponentDetails": [ { "size":"1 x 0.3 mL", "name":"Mouse IgG standard 0.1 µg/ml", "number":"AB273150-CMP03" }, { "size":"1 x 0.3 mL", "name":"Mouse IgG standard 1.9 µg/ml", "number":"AB273150-CMP06" }, { "size":"1 x 0.3 mL", "name":"Mouse IgG standard 0.75 µg/ml", "number":"AB273150-CMP05" }, { "size":"1 x 0.3 mL", "name":"Mouse IgG standard 0.2 µg/ml", "number":"AB273150-CMP04" }, { "size":"1 x 0.3 mL", "name":"Mouse IgG standard 0.02 µg/ml", "number":"AB273150-CMP02" }, { "size":"1 x 12 mL", "name":"Detection antibody", "number":"AB273150-CMP11" }, { "size":"1 x 30 mL", "name":"Sample Diluent", "number":"AB273150-CMP08" }, { "size":"1 x 0.3 mL", "name":"Mouse IgG standard 0.0 µg/ml", "number":"AB273150-CMP01" }, { "size":"1 x 12 mL", "name":"TMB Substrate", "number":"AB273150-CMP07" }, { "size":"1 x 96 Test", "name":"Pre-coated 8 microwells strips", "number":"AB273150-CMP10" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB273150-CMP09" } ] }, "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab273150-10x96Tests", "assetComponentDetails": [ { "size":"1 x 1 mL", "name":"Mouse IgG standard 0.1 µg/ml", "number":"AB273150-CMP03", "productcode":"" }, { "size":"1 x 1 mL", "name":"Mouse IgG standard 1.9 µg/ml", "number":"AB273150-CMP06", "productcode":"" }, { "size":"1 x 1 mL", "name":"Mouse IgG standard 0.75 µg/ml", "number":"AB273150-CMP05", "productcode":"" }, { "size":"1 x 1 mL", "name":"Mouse IgG standard 0.2 µg/ml", "number":"AB273150-CMP04", "productcode":"" }, { "size":"1 x 1 mL", "name":"Mouse IgG standard 0.02 µg/ml", "number":"AB273150-CMP02", "productcode":"" }, { "size":"1 x 1 mL", "name":"Mouse IgG standard 0.0 µg/ml", "number":"AB273150-CMP01", "productcode":"" }, { "size":"1 x 120 mL", "name":"Detection antibody", "number":"AB273150-CMP11", "productcode":"" }, { "size":"10 x 96 Test", "name":"Pre-coated 8 microwells strips", "number":"AB273150-CMP10", "productcode":"" }, { "size":"1 x 500 mL", "name":"Sample Diluent", "number":"AB273150-CMP08", "productcode":"" }, { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB273150-CMP09", "productcode":"" }, { "size":"1 x 120 mL", "name":"TMB Substrate", "number":"AB273150-CMP07", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column
style
left-column

Product protocols

style
left-column

Target data

style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Assay Kits

AB214568

Human IgM ELISA Kit

SimpleStep

ELISA - Human IgM ELISA Kit (AB214568)

  • 0
  • 0
Assay Kits

AB196263

Human IgA ELISA Kit

Recombinant|SimpleStep

ELISA - Human IgA ELISA Kit (AB196263)

  • 4
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Assay Kits

AB189577

Chicken IgY ELISA Kit

SimpleStep

ELISA - Chicken IgY ELISA Kit (AB189577)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com