Mouse IgG ELISA Kit

Product details

Mouse IgG ELISA Kit ab151276 is a sandwich ELISA to measure mouse immunoglobulin G (IgG) in cell culture media, mouse IgG, and serum with a sensitivity of 2.2 ng/mL.

How the assay works

This assay employs a mouse IgG specific antibody coated onto plate strips. Standards and samples are pipetted into the wells and IgG present in the sample is bound to the wells by the immobilized antibody. The wells are washed and an HRP-conjugated anti-mouse IgG detector antibody is added. After washing away unbound detector antibody, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IgG bound. The developing blue color is measured at 600 nm. Optionally the reaction can be stopped with the Stop Solution which changes the color from blue to yellow and the intensity can be measured at 450 nm.

Assay protocol summary

Mouse IgG ELISA Kit ab151276 protocol summary:

1. Add samples and standards to wells. Incubate at room temperature.
2. Wash each well and add HRP-labeled detector antibody. Incubate at room temperature.
3. Wash each well and add HRP Development Solution. Incubate at room temperature.
4. Add Stop Solution to each well. Read immediately.

Getting the best performance

ASSAY SPECIFICITY

This kit specifically reactive with mouse IgG, but also weakly reactive with rat serum. Not reactive with bovine serum or goat serum. Other species untested.

Target information

There are five classes of mammalian immunoglobulins: IgA, IgD, IgE, IgM, and IgG. IgG is the most abundant immunoglobulin and is equally distributed in blood and tissue. In mice, the IgG class is further divided into four subclasses: IgG1, IgG2a/ IgG2c (strain specific), IgG2b, and IgG3. The general immunoglobulin structure is composed of four polypeptide chains, two heavy and two light chains linked together and to each other by disulfide bonds, creating a tetrameric quaternary structure. The resulting tetramer creates two identical halves which together form a Y like structure. While the amino-terminal portions that exhibits highly variable amino-acid composition are involved in antigen binding, the C terminal constant parts are involved in complement binding, placental passage and binding to cell membrane. IgG is involved in response to a foreign antigen. The presence of IgG usually signifies a mature antibody response. IgG has a molecular weight of about 150 kDa, it can bind to many pathogens and also plays an important role in antibody-dependent cell-mediated cytotoxicity. Typically mouse serum and plasma samples contain about 7 to 10 mg/ml of IgG.

How other researchers are using this kit

Mouse IgG ELISA Kit ab151276 has been used to study autoimmune diseases and infections in various physio-pathological contexts such as lupus (1) and arthritis (2).

References: 1-Bailing Tian et al. 2022, PMID: 36398225, 2-Miaojia Zhang et al. 2021, PMID: 33815378

Related and recommended products

Mouse IgG ELISA Kit ab151276 is commonly used for the quantification of IgG to measure immune system functioning.

Other ELISA kits to measure Immunoglobulins include:

-Mouse/Rat IgM ELISA Kit ab215085
-Mouse IgA ELISA Kit ab314603
-Mouse IgE ELISA Kit- Intracellular ab317774
-Mouse IgE ELISA Kit- Extracellular ab317773

Mouse IgG ELISA Kit ab151276 is a sandwich ELISA to measure mouse immunoglobulin G in cell culture media, serum and plasma with a sensitivity of 2.2 ng/mL.

How the assay works
This assay employs a mouse IgG specific antibody coated onto plate strips. Standards and samples are pipetted into the wells and IgG present in the sample is bound to the wells by the immobilized antibody. The wells are washed and an HRP-conjugated anti-mouse IgG detector antibody is added. After washing away unbound detector antibody, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IgG bound. The developing blue color is measured at 600 nm. Optionally the reaction can be stopped with the Stop Solution which changes the color from blue to yellow and the intensity can be measured at 450 nm.

Assay specificity
This kit specifically reactive with mouse IgG, but also weakly reactive with rat serum. Not reactive with bovine serum or goat serum. Other species untested.

Mouse IgG ELISA Kit ab151276 protocol summary
1. Add samples and standards to wells. Incubate at room temperature
2. Wash each well and add HRP-labeled detector antibody. Incubate at room temperature
3. Wash each well and add HRP Development Solution. Incubate at room temperature
4. Add Stop Solution to each well. Read immediately.

How other researchers are using Mouse IgG ELISA Kit ab151276
Mouse IgG ELISA Kit ab151276 has been used to study autoimmune diseases and infections in various physio-pathological contexts such as lupus⁽¹⁾ and arthritis ⁽²⁾.

References:
⁽¹⁾Bailing Tian et al. 2022, PMID: 36398225,
⁽²⁾Miaojia Zhang et al. 2021, PMID: 33815378

Related and recommended products
Mouse IgG ELISA Kit ab151276 is commonly used for the quantification of IgG to measure immune system functioning.

Other ELISA kits to measure Immunoglobulins include:
- Mouse/Rat IgM ELISA Kit ab215085
- Mouse IgA ELISA Kit ab314603
- Mouse IgE ELISA Kit- Intracellular ab317774
- Mouse IgE ELISA Kit- Extracellular ab317773