AB157719
Mouse IgG ELISA Kit is a sandwich ELISA designed to quantify Mouse IgG with a sensitivity of 2.374 ng/mL.
- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 6.25 - 200 ng/mL
- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 6.25 - 200 ng/mL
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- sELISA
Supplier Data
Sandwich ELISA - Mouse IgG ELISA Kit (AB157719)
Representative standard curve using ab157719.
Reactivity data
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Product details
Mouse IgG ELISA Kit ab157719 is a sandwich ELISA to measure Mouse IgG in serum, plasma, other biological fluids with a sensitivity of 2.374 ng/ml.
How the assay works
In this assay the IgG present in samples reacts with the anti-IgG antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgG antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgG. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgG in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgG in the test sample. The quantity of IgG in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Mouse IgG ELISA Kit ab157719 protocol summary
1. Add standard or sample to each well used. Incubate at room temperature.
2. Aspirate and wash each well.
3. Add prepared HRP labeled secondary detector antibody. Incubate at room temperature
4. Aspirate and wash each well.
5. Add Chromogen Substrate Solution to each well.
6. Immediately begin recording the color development
How the assay works
In this assay the IgG present in samples reacts with the anti-IgG antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgG antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgG. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgG in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgG in the test sample. The quantity of IgG in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Mouse IgG ELISA Kit ab157719 protocol summary
1. Add standard or sample to each well used. Incubate at room temperature.
2. Aspirate and wash each well.
3. Add prepared HRP labeled secondary detector antibody. Incubate at room temperature
4. Aspirate and wash each well.
5. Add Chromogen Substrate Solution to each well.
6. Immediately begin recording the color development
Precision
[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
Recovery
[ { "sample": "Serum", "range": null, "average": "> 85" } ]
What's included?
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Properties and storage information
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Publications (7)
Recent publications for all applications. Explore the full list and refine your search
Nutrients 16: PubMed39339746
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Botanical Bioflavonoid Composition from - and -Protected Mice against D-Galactose-Induced Immunosenescence, and Cyclophosphamide Induced Immune Suppression.
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Mesfin Yimam,Teresa Horm,Alexandria O'Neal,Paola Chua,Ping Jiao,Mei Hong,Qi Jia
Antioxidants (Basel, Switzerland) 12: PubMed37107267
2023
Antioxidant and Immune Stimulating Effects of Skin in the RAW 264.7 Cells and in the C57BL/6 Mouse Immunosuppressed by Cyclophosphamide.
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Ji-Su Kim,Eun-Byeol Lee,Ji-Hye Choi,Jieun Jung,Un-Yul Jeong,Ui-Jin Bae,Hwan-Hee Jang,Shin-Young Park,Youn-Soo Cha,Sung-Hyen Lee
Molecular therapy. Methods & clinical development 26:505-518 PubMed36092367
2022
AAV-monoclonal antibody expression protects mice from Ebola virus without impeding the endogenous antibody response to heterologous challenge.
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Laura P van Lieshout,Amira D Rghei,Wenguang Cao,Shihua He,Geoff Soule,Wenjun Zhu,Sylvia P Thomas,Debra Sorensen,Kathy Frost,Kevin Tierney,Brad Thompson,Stephanie Booth,David Safronetz,Raveendra R Kulkarni,Byram W Bridle,Xiangguo Qiu,Logan Banadyga,Sarah K Wootton
Frontiers in immunology 12:693608 PubMed34367152
2021
Single-Cell RNA Sequencing Reveals the Immunological Profiles of Renal Allograft Rejection in Mice.
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Qixia Shen,Yucheng Wang,Jiaoyi Chen,Lifeng Ma,Xiaoru Huang,Sydney C W Tang,Huiyao Lan,Hong Jiang,Jianghua Chen
Acta pharmaceutica (Zagreb, Croatia) 70:483-498 PubMed32412434
2020
Synthesis and evaluation of bisulfate/mesylate-conjugated chlortetracycline with high solubility and bioavailability.
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Deng Liu,Peixing Chen,Xu Yang,Jianhua Wang
Nature 577:543-548 PubMed31915378
2020
Microbiota-targeted maternal antibodies protect neonates from enteric infection.
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Wen Zheng,Wenjing Zhao,Meng Wu,Xinyang Song,Florence Caro,Ximei Sun,Francesca Gazzaniga,Giuseppe Stefanetti,Sungwhan Oh,John J Mekalanos,Dennis L Kasper
Science advances 5:eaaw4357 PubMed31309150
2019
Charge-switchable polymeric complex for glucose-responsive insulin delivery in mice and pigs.
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Jinqiang Wang,Jicheng Yu,Yuqi Zhang,Xudong Zhang,Anna R Kahkoska,Guojun Chen,Zejun Wang,Wujin Sun,Lulu Cai,Zhaowei Chen,Chenggen Qian,Qundong Shen,Ali Khademhosseini,John B Buse,Zhen Gu
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