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AB273149

Mouse IgG Isotyping Kit

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(1 Publication)

Mouse IgG Isotyping Kit is a ELISA for the measurement of Mouse IgG Isotyping Kit in in samples.

Key facts

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Product details

The Mouse IgG Isotyping Kit (ab273149) provides a rapid and easy method (one step ELISA) to characterize mouse monoclonal antibody isotypes in cell culture supernatants or purified antibodies preparations. The kit includes ready-to-use reagents necessary to analyze 12 samples in less than 30 minutes.

Anti-mouse antibodies specific to each of the common light and heavy chains are pre-coated in the wells. Samples are pipetted into microwells and Ig present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-mouse IgG (H+L) antibody is pipetted and incubated simultaneously with samples. After washing microwells in order to remove any non specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops if the specific immunoglobulin is present in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 14:1237498 PubMed37691918

2023

Multi-omics analysis identifies IgG2b class-switching with ALCAM-CD6 co-stimulation in joint-draining lymph nodes during advanced inflammatory-erosive arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

H Mark Kenney,Javier Rangel-Moreno,Yue Peng,Kiana L Chen,Jennifer Bruno,Abdul Embong,Elizabeth Pritchett,Jeffrey I Fox,Enrique Becerril-Villanueva,Armando Gamboa-Domínguez,Sally Quataert,Gowrishankar Muthukrishnan,Ronald W Wood,Benjamin D Korman,Jennifer H Anolik,Lianping Xing,Christopher T Ritchlin,Edward M Schwarz,Chia-Lung Wu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com