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AB133045

Mouse IgG1 ELISA Kit

3

(1 Review)

|

(8 Publications)

Mouse IgG1 ELISA Kit is a sandwich ELISA designed to quantify Mouse IgG1 with a sensitivity of 0.116 ng/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 7.81 - 250 ng/mL
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Sandwich ELISA - Mouse IgG1 ELISA Kit (AB133045)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IgG1 ELISA Kit (AB133045)

Representative Standard Curve using ab133045.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 0.116 ng/mL

Range

7.81 - 250 ng/mL

Assay time

90m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse IgG1 ELISA Kit ab133045 is a sandwich ELISA to measure Mouse IgG1 in serum, cell culture supernatant with a sensitivity of 0.116 ng/ml.

How the assay works

IgG1 specific antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and along with an HRP-conjugated IgG1 detection antibody and the microplate is then incubated at room temperature. After the removal of unbound proteins by washing, TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a colored product that changes after adding acidic stop solution. The density of coloration is directly proportional to the IgG1 amount of sample captured in plate.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Mouse IgG1 ELISA Kit ab133045 protocol summary

1. Add standard or sample to each well used. Incubate at room temperature.
2. Aspirate and wash each well.
3. Add prepared HRP labeled secondary detector antibody. Incubate at room temperature
4. Aspirate and wash each well.
5. Add TMB Substrate Solution to each well.
6. Immediately begin recording the color development

IgG is divided into four subclasses; IgG1, IgG2, IgG3, and IgG4. IgG1 is the most abundant immunoglobulin found in the blood. It is a glycoprotein which consists of two identical heavy chains (50 kDa each) and two identical light chains (25 kDa each), to give a combined mass of approximately 150 kDa. The chains are held in place by covalent disulfide bonds. Each light chain contains two immunoglobulin (Ig) domains, while the heavy chains contain four Ig domains each. In the middle of each heavy chain is a relative varying portion called the "hinge region" which is unique to each IgG. This region allows for molecular flexibility and sets IgG1 apart from its IgG counterparts. IgG1 properties and functions include neutralization, opsonization, activation of the complement system, diffusion into extravascular sites and crossing the placenta.

Cross Reactivity

Compound Cross Reactivity
Rat IgG1 0.9%
Mouse IgG2b 0.21
Human IgG1 <0.01%
Mouse IgG2a <0.01%
Mouse IgG3 <0.01%
Mouse IgM <0.01%

Recommended dilution of serum is 1:20,000

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Precision

[ { "reproducibilityType": "Inter", "sample": "High", "replicates": 16, "mean": "148.7 ng/mL", "standardDeviation": null, "coefficientOfVariability": "8.1" }, { "reproducibilityType": "Inter", "sample": "Low", "replicates": 16, "mean": "48.5 ng/mL", "standardDeviation": null, "coefficientOfVariability": "3.1" }, { "reproducibilityType": "Inter", "sample": "Media", "replicates": 16, "mean": "98.3 ng/mL", "standardDeviation": null, "coefficientOfVariability": "4.4" }, { "reproducibilityType": "Intra", "sample": "High", "replicates": 19, "mean": "160.4 ng/mL", "standardDeviation": null, "coefficientOfVariability": "3.6" }, { "reproducibilityType": "Intra", "sample": "Low", "replicates": 19, "mean": "49.9 ng/mL", "standardDeviation": null, "coefficientOfVariability": "1.2" }, { "reproducibilityType": "Intra", "sample": "Media", "replicates": 19, "mean": "99.6 ng/mL", "standardDeviation": null, "coefficientOfVariability": "2.7" } ]
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Recovery

[ { "sample": "Serum", "range": null, "average": "= 102.8" }, { "sample": "Tissue Culture Media", "range": null, "average": "= 105.3" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Science advances 7:eabj5830 PubMed34851674

2021

Biomaterials direct functional B cell response in a material-specific manner.

Applications

Unspecified application

Species

Unspecified reactive species

Erika M Moore,David R Maestas,Chris C Cherry,Jordan A Garcia,Hannah Y Comeau,Locke Davenport Huyer,Sean H Kelly,Alexis N Peña,Richard L Blosser,Gedge D Rosson,Jennifer H Elisseeff

Cell reports 36:109372 PubMed34260908

2021

Inhibition of Rag GTPase signaling in mice suppresses B cell responses and lymphomagenesis with minimal detrimental trade-offs.

Applications

Unspecified application

Species

Unspecified reactive species

Ana Ortega-Molina,Cristina Lebrero-Fernández,Alba Sanz,Nerea Deleyto-Seldas,Ana Belén Plata-Gómez,Camino Menéndez,Osvaldo Graña-Castro,Eduardo Caleiras,Alejo Efeyan

Frontiers in immunology 11:575504 PubMed33117374

2020

Combinational PRR Agonists in Liposomal Adjuvant Enhances Immunogenicity and Protective Efficacy in a Tuberculosis Subunit Vaccine.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Hao,Yaqi Wu,Yandi Zhang,Zijie Zhou,Qing Lei,Nadeem Ullah,Jo-Lewis Banga Ndzouboukou,Xiaosong Lin,Xionglin Fan

Journal of immunology research 2020:2083793 PubMed32953889

2020

Differential Immunogenicity and Protective Efficacy Elicited by MTO- and DMT-Adjuvanted CMFO Subunit Vaccines against Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Nadeem Ullah,Ling Hao,Yaqi Wu,Yandi Zhang,Qing Lei,Jo-Lewis Banga Ndzouboukou,Xiaosong Lin,Xionglin Fan

Nature metabolism 1:775-789 PubMed31579886

2019

Oncogenic Rag GTPase signaling enhances B cell activation and drives follicular lymphoma sensitive to pharmacological inhibition of mTOR.

Applications

Unspecified application

Species

Unspecified reactive species

Ana Ortega-Molina,Nerea Deleyto-Seldas,Joaquim Carreras,Alba Sanz,Cristina Lebrero-Fernández,Camino Menéndez,Andrew Vandenberg,Beatriz Fernández-Ruiz,Leyre Marín-Arraiza,Celia de la Calle Arregui,Ana Belén Plata-Gómez,Eduardo Caleiras,Alba de Martino,Nuria Martínez-Martín,Kevin Troulé,Elena Piñeiro-Yáñez,Naoya Nakamura,Shamzah Araf,Gabriel D Victora,Jessica Okosun,Jude Fitzgibbon,Alejo Efeyan

Journal of ethnopharmacology 224:15-26 PubMed29787795

2018

Anti-allergy and anti-tussive activity of Clitoria ternatea L. in experimental animals.

Applications

Unspecified application

Species

Unspecified reactive species

Niraj Kumar Singh,Debapriya Garabadu,Priyanka Sharma,Sushant Kumar Shrivastava,Pradeep Mishra

EBioMedicine 22:143-154 PubMed28711483

2017

A Multistage Subunit Vaccine Effectively Protects Mice Against Primary Progressive Tuberculosis, Latency and Reactivation.

Applications

Unspecified application

Species

Unspecified reactive species

Jilei Ma,Xindong Teng,Xiaochun Wang,Xionglin Fan,Yaqi Wu,Maopeng Tian,Zijie Zhou,Longmeng Li

Virology journal 11:142 PubMed25107552

2014

Development of a reverse genetics system for respiratory syncytial virus long strain and an immunogenicity study of the recombinant virus.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Hu,Jiawei Jiang,Jianbo Zhan,Guoming Li,Yongzhong Jiang,Xuhua Guan,Yuanding Chen,Zhizheng Fang
View all publications
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