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AB316264

Mouse IgG1 ELISA Kit

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Mouse IgG1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse IgG1 with a sensitivity of 40.7 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Competitive ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form

3 Images
Competitive ELISA - Mouse IgG1 ELISA Kit (AB316264)
  • cELISA

Supplier Data

Competitive ELISA - Mouse IgG1 ELISA Kit (AB316264)

Interpolated concentrations of native IgG1 were measured in duplicate at different sample concentrations. Undiluted samples are 1 : 1000 serum, 1 : 500 plasma (citrate), 1 : 500 plasma (EDTA), 1 : 500 plasma (heparin). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Competitive ELISA - Mouse IgG1 ELISA Kit (AB316264)
  • cELISA

Supplier Data

Competitive ELISA - Mouse IgG1 ELISA Kit (AB316264)

Recombinant IgG1 was spiked into the following samples and diluted in a 2-fold dilution series in Sample Diluent NS. Undiluted samples are 50% urine and cell culture media. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Competitive ELISA - Mouse IgG1 ELISA Kit (AB316264)
  • cELISA

Supplier Data

Competitive ELISA - Mouse IgG1 ELISA Kit (AB316264)

Example of mouse IgG1 standard curve. OD (450 nm) values (mean +/- SD) are graphed

Key facts

Detection method

Colorimetric

Sample types

Serum, EDTA Plasma, Cell culture media, Urine, Heparin Plasma, Citrate plasma

Reacts with

Mouse

Assay type

Competitive

Results type

Quantitative

Sensitivity

= 40.7 ng/mL

Range

62.5 - 2000 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse IgG1 SimpleStep ELISA® kit is a single-wash 90 min competitive ELISA designed for the quantitative measurement of IgG1 in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture media, and Urine. Quantitate Mouse IgG1 with 40.7 ng/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
        -High sensitivity, specificity and reproducibility from superior antibodies
        -Fully validated in biological samples
        -96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Citrate plasma", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 9.8" }, { "reproducibilityType": "Inter", "sample": "Citrate plasma", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 8.93" } ]
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Recovery

[ { "sample": "Serum", "range": "100 - 114 %", "average": "= 106" }, { "sample": "Citrate plasma", "range": "95 - 123 %", "average": "= 107" }, { "sample": "EDTA Plasma", "range": "95 - 97 %", "average": "= 96" }, { "sample": "Heparin Plasma", "range": "89 - 101 %", "average": "= 96" }, { "sample": "Cell culture media", "range": "89 - 102 %", "average": "= 93" }, { "sample": "Urine", "range": "89 - 113 %", "average": "= 101" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

See full target information Ighg1
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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