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AB157720

Mouse IgG2c ELISA Kit

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(3 Publications)

Mouse IgG2c ELISA Kit is a Sandwich ELISA for the measurement of Mouse IgG2c in Mouse in Biofluids samples.
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ELISA - Mouse IgG2c ELISA Kit (AB157720)
  • ELISA

Supplier Data

ELISA - Mouse IgG2c ELISA Kit (AB157720)

Representative standard curve using ab157720 IgG2c Mouse ELISA Kit

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.38 ng/mL

Range

3.13 - 200 ng/mL

Assay Platform

Microplate

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Secondary Antibodies

AB97255

Goat Anti-Mouse IgG2c heavy chain (HRP)

  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's IgG2c Mouse ELISA kit is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of IgG2c in mouse samples.

In this assay the IgG2c present in samples reacts with the anti-IgG2c antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgG2c antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgG2c. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgG2c in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgG2c in the test sample. The quantity of IgG2c in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": null, "average": "> 85" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab157720-1x96Tests", "assetComponentDetails": [ { "size":"1 x 150 µL", "name":"100X HRP-conjugated anti-mouse IgG2c antibody", "number":"AB157720-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Mouse IgG2c Calibrator (lyophilized)", "number":"AB157720-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Mouse IgG2c ELISA Microplate", "number":"AB157720-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Chromogen Substrate Solution", "number":"AB157720-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB157720-CMP01", "productcode":"" }, { "size":"1 x 50 mL", "name":"20X Wash Buffer Concentrate", "number":"AB157720-CMP06", "productcode":"" }, { "size":"1 x 50 mL", "name":"5X Diluent Concentrate", "number":"AB157720-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 11:575504 PubMed33117374

2020

Combinational PRR Agonists in Liposomal Adjuvant Enhances Immunogenicity and Protective Efficacy in a Tuberculosis Subunit Vaccine.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Hao,Yaqi Wu,Yandi Zhang,Zijie Zhou,Qing Lei,Nadeem Ullah,Jo-Lewis Banga Ndzouboukou,Xiaosong Lin,Xionglin Fan

Journal of immunology research 2020:2083793 PubMed32953889

2020

Differential Immunogenicity and Protective Efficacy Elicited by MTO- and DMT-Adjuvanted CMFO Subunit Vaccines against Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Nadeem Ullah,Ling Hao,Yaqi Wu,Yandi Zhang,Qing Lei,Jo-Lewis Banga Ndzouboukou,Xiaosong Lin,Xionglin Fan

EBioMedicine 22:143-154 PubMed28711483

2017

A Multistage Subunit Vaccine Effectively Protects Mice Against Primary Progressive Tuberculosis, Latency and Reactivation.

Applications

Unspecified application

Species

Unspecified reactive species

Jilei Ma,Xindong Teng,Xiaochun Wang,Xionglin Fan,Yaqi Wu,Maopeng Tian,Zijie Zhou,Longmeng Li
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Product promise

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For full details, please see our Terms & Conditions

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