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AB113344

Mouse IL-1 alpha ELISA Kit

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(5 Publications)

Mouse IL-1 alpha ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse IL-1 alpha in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Interleukin-1 alpha, IL-1 alpha, Il1a

1 Images
Sandwich ELISA - Mouse IL-1 alpha ELISA Kit (AB113344)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-1 alpha ELISA Kit (AB113344)

Representative Standard Curve using ab113344

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

< 10 pg/mL

Range

12.23 - 3000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's IL-1 alpha Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IL-1 alpha in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Mouse IL-1 alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse IL-1 alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "104 - 123 %", "average": "= 113.6" }, { "sample": "Serum", "range": "113 - 132 %", "average": "= 122.4" }, { "sample": "Plasma", "range": "77 - 140 %", "average": "= 112.3" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab113344-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB113344-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB113344-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse IL-1 alpha Standard (lyophilized)", "number":"AB113344-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D", "number":"AB113344-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB113344-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IL-1 alpha Microplate (12 x 8 wells)", "number":"AB113344-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Mouse IL-1 alpha", "number":"AB113344-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB113344-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin Concentrate", "number":"AB113344-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Cytokine constitutively present intracellularly in nearly all resting non-hematopoietic cells that plays an important role in inflammation and bridges the innate and adaptive immune systems (PubMed : 16256210). After binding to its receptor IL1R1 together with its accessory protein IL1RAP, forms the high affinity interleukin-1 receptor complex. Signaling involves the recruitment of adapter molecules such as MYD88, IRAK1 or IRAK4. In turn, mediates the activation of NF-kappa-B and the three MAPK pathways p38, p42/p44 and JNK pathways (PubMed : 1386364). Within the cell, acts as an alarmin and cell death results in its liberation in the extracellular space after disruption of the cell membrane to induce inflammation and alert the host to injury or damage. In addition to its role as a danger signal, which occurs when the cytokine is passively released by cell necrosis, directly senses DNA damage and acts as a signal for genotoxic stress without loss of cell integrity (By similarity).
See full target information IL1A_MOUSE
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cell biology and toxicology 41:33 PubMed39825191

2025

Deciphering SPP1-related macrophage signaling in the pathogenesis of intervertebral disc degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Jun Yu,Peng Zou,Tian-Qi Li,Xiao-Fan Bai,Shan-Xi Wang,Jian-Bin Guan,Yuan-Ting Zhao,Meng-Wei Li,Xiaodong Wang,Ying-Guang Wang,Ding-Jun Hao

Frontiers in cell and developmental biology 10:794198 PubMed35252176

2022

Therapeutic Effects of Kefir Peptides on Hemophilia-Induced Osteoporosis in Mice With Deficient Coagulation Factor VIII.

Applications

Unspecified application

Species

Unspecified reactive species

Chih-Ching Yen,Yao-Wen Liu,Gary Ro-Lin Chang,Ying-Wei Lan,Yung-Tsung Kao,Shin-Nan Cheng,Wei Chen,Chuan-Mu Chen

CNS neuroscience & therapeutics 27:206-219 PubMed33112032

2020

Activation of dopaminergic VTA inputs to the mPFC ameliorates chronic stress-induced breast tumor progression.

Applications

Unspecified application

Species

Unspecified reactive species

Xi-Rong Xu,Qian Xiao,Yu-Chuan Hong,Yun-Hui Liu,Yue Liu,Jie Tu

Molecular cell 68:185-197.e6 PubMed28943315

2017

NLRP3 Phosphorylation Is an Essential Priming Event for Inflammasome Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Nan Song,Zhao-Shan Liu,Wen Xue,Zhao-Fang Bai,Qian-Yi Wang,Jiang Dai,Xin Liu,Yi-Jiao Huang,Hong Cai,Xiao-Yan Zhan,Qiu-Ying Han,Hongxia Wang,Yuan Chen,Hui-Yan Li,Ai-Ling Li,Xue-Min Zhang,Tao Zhou,Tao Li

Mucosal immunology 11:257-272 PubMed28612844

2017

NLRP3 inflammasome pathway has a critical role in the host immunity against clinically relevant Acinetobacter baumannii pulmonary infection.

Applications

Unspecified application

Species

Unspecified reactive species

N Dikshit,S D Kale,H J Khameneh,V Balamuralidhar,C Y Tang,P Kumar,T P Lim,T T Tan,A L Kwa,A Mortellaro,B Sukumaran
View all publications
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