AB100704

Mouse IL-1 beta ELISA Kit

Name: Mouse IL-1 beta ELISA Kit (ab100704) | Abcam
Brand: Abcam Limited
SKU: AB100704

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(76 Publications)

Mouse IL-1 beta ELISA Kit is a Sandwich ELISA for the measurement of Mouse IL-1 beta in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Interleukin-1 beta, IL-1 beta, Il1b

5 Images

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB100704)

sELISA

Unknown

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB100704)

Representative standard curve using ab100704

sELISA

Unknown

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB100704)

Standard curve of msIL-1b in different diluents with background signal subtracted (duplicates; +/- SD).

sELISA

Unknown

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB100704)

Representative standard curve using ab100704

sELISA

Unknown

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB100704)

IL-1b measured in mouse tissue lysates (expressed as per mg of extracted protein), with background signal subtracted (duplicates; +/- SD).

sELISA

Unknown

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB100704)

IL-1b detected in supernatants from RAW 246.7 control cells (C) or cells stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297) (P), or with PMA and 1 ug x mL-1 of LPS (Sigma) (P+L) for the last 6 hours. Results shown after background signal was subtracted (duplicates +/- SD).

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 5 pg/mL

Range

2.74 - 2000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Abcam's IL-1 beta Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-1 beta in plasma and cell culture supernatants.

This assay employs an antibody specific for mouse IL-1 beta coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-1 beta antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

We have not been able to detect the endogenous Mouse IL-1 beta in normal serum with ab100704, only in serum spiked with Mouse IL-1 beta.

Get higher sensitivity in only 90 minutes with Mouse IL-1 beta ELISA Kit (ab197742) from our SimpleStep ELISA^® range.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "89 - 110 %", "average": "= 98.64" }, { "sample": "Plasma", "range": "90 - 109 %", "average": "= 99.24" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100704-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100704-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100704-CMP07", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100704-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100704-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB100704-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse IL-1 beta Standard (lyophilized)", "number":"AB100704-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-mouse IL-1 beta", "number":"AB100704-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IL-1 beta Microplate (12 x 8 wells)", "number":"AB100704-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100704-CMP08", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Product protocols

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Target data

Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis : its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore.

See full target information Il1b

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Publications (76)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 16:1567210 PubMed40160456

2025

The protective role of cannabidiol in stress-induced liver injury: modulating oxidative stress and mitochondrial damage.

Applications

Unspecified application

Species

Unspecified reactive species

Chengyu Huang,Huichao Liang,Xiaohua Liang,Yueyi Liu,Jiaoling Wang,Haoran Jiang,Xinhui Kou,Jun Chen,Lili Huang

Heliyon 9:e20124 PubMed37771539

2023

IL-1β-pretreated bone mesenchymal stem cell-derived exosomes alleviate septic endoplasmic reticulum stress via regulating SIRT1/ERK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xinsheng Cheng,Shikai Wang,Zhipeng Li,Di He,Jianguo Wu,Weiwei Ding

Thrombosis journal 21:44 PubMed37076891

2023

Long non-coding RNA crnde promotes deep vein thrombosis by sequestering miR-181a-5p away from thrombogenic Pcyox1l.

Applications

Unspecified application

Species

Unspecified reactive species

Xin He,Yu Liu,Yaozhen Li,Kemin Wu

Allergologia et immunopathologia 51:159-167 PubMed36617836

2023

TRIM52 aggravated inflammation and pyroptosis in dextran sulfate sodium-induced inflammatory bowel disease through activation of the TLR4/NF-κBs pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jin-Ping Ma,Cheng-Zi Yao,Zhi-Qiang Jia,A-Mei Wang,Xiang-Xia Miao,Xiang-Xiang Gao,Li-Ping Su

Allergologia et immunopathologia 50:84-90 PubMed36086968

2022

Knockdown of EDA2R alleviates hyperoxia-induced lung epithelial cell injury by inhibiting NF-κB pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Nan Jia,Yi Jia,Fen Yang,Wenchao Du

BMC pharmacology & toxicology 23:58 PubMed35906691

2022

Pharmacological evaluation of newly synthesized organotin IV complex for antiulcer potential.

Applications

Unspecified application

Species

Unspecified reactive species

Syed Azmatullah,Arif-Ullah Khan,Neelam Gul Qazi,Humaira Nadeem,Nadeem Irshad

Frontiers in immunology 13:941219 PubMed35911675

2022

Cold Plasma Irradiation Attenuates Atopic Dermatitis Enhancing HIF-1α-Induced MANF Transcription Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Sun,Xinru Zhang,Chao Hou,Shujun Yu,Yujing Zhang,Zhuo Yu,Ling Kong,Changqing Liu,Lijie Feng,Dong Wang,Guohua Ni

Frontiers in cellular and infection microbiology 12:839592 PubMed35719341

2022

Fenofibrate Ameliorated Systemic and Retinal Inflammation and Modulated Gut Microbiota in High-Fat Diet-Induced Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xue Wang,Chaofeng Yu,Xiaomei Liu,Jiasong Yang,Yuliang Feng,Yajun Wu,Yali Xu,Yihua Zhu,Wensheng Li

Journal of neuroinflammation 19:122 PubMed35624490

2022

Intermittent fasting reduces neuroinflammation in intracerebral hemorrhage through the Sirt3/Nrf2/HO-1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shuhui Dai,Jialiang Wei,Hongchen Zhang,Peng Luo,Yuefan Yang,Xiaofan Jiang,Zhou Fei,Wenbin Liang,Jianli Jiang,Xia Li

The Kaohsiung journal of medical sciences 38:565-573 PubMed35579106

2022

microRNA-145-5p attenuates acute lung injury via targeting ETS2.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Qiao,Rong-Xia Li,Shan-Gang Hu,Yu Liu,Hong-Qiang Liu,Hong-Jun Wu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse IL-1 beta ELISA Kit

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB100704)

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB100704)

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB100704)

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB100704)

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB100704)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay Platform

Complementary Products

Assay Kits

Assay Kits

Secondary Antibodies

Assay Kits

Reactivity data

Product details

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (76)

Product promise