AB100705

Mouse IL-1 beta ELISA Kit

Name: Mouse IL-1 beta ELISA Kit (ab100705) | Abcam
Brand: Abcam Limited
SKU: AB100705
Price: 780 USD
Availability: InStock
Rating: 4 (3 reviews)

(3 Reviews)

(25 Publications)

Mouse IL-1 beta ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse IL-1 beta in Mouse in Cell/Tissue Extracts samples.

View Alternative Names

Interleukin-1 beta, IL-1 beta, Il1b

4 Images

ELISA - Mouse IL-1 beta ELISA Kit (AB100705)

ELISA

Lab

ELISA - Mouse IL-1 beta ELISA Kit (AB100705)

IL-1 beta measured in lysates from control cells, or cells stimulated for 24 hours with 50 ng x mL^-1 of PMA (ab120297), with the addition of 1 ug x mL^-1 of LPS (Sigma) (P/L) for the last 6 hours (duplicates +/- SD).

ELISA

Lab

ELISA - Mouse IL-1 beta ELISA Kit (AB100705)

Dilution curves of mouse IL-1 beta (ab100705) and NIBSC standard (93/668). One ng of standard mouse IL-1 beta corresponds to 34.6 (+/-2.1) AU NIBSC 93/668. Background signal subtracted (duplicates; +/- SD).

ELISA

Lab

ELISA - Mouse IL-1 beta ELISA Kit (AB100705)

IL-1 beta measured in mouse tissue lysates (0.02-0.3 mg x mL^-1 protein tested, data expressed per

mg of extracted protein; duplicates +/- SD).

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB100705)

sELISA

Unknown

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB100705)

Representative standard curve using ab100705

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

< 5 pg/mL

Range

2.74 - 2000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Abcam's IL-1 beta Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-1 beta in cell lysates and tissue lysates.

This assay employs an antibody specific for mouse IL-1 beta coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-1 beta antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Get higher sensitivity in only 90 minutes with Mouse IL-1 beta ELISA Kit (ab197742) from our SimpleStep ELISA^® range.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Recovery

[ { "sample": "Cell culture extracts", "range": "90 - 111 %", "average": "= 102.34" }, { "sample": "Tissue Extracts", "range": "89 - 109 %", "average": "= 98.26" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100705-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100705-CMP04", "productcode":"" }, { "size":"1 x 5 mL", "name":"2X Cell Lysis Buffer", "number":"AB100705-CMP09", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100705-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse IL-1 beta Standard (lyophilized)", "number":"AB100705-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100705-CMP08", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Sample Diluent Buffer", "number":"AB100705-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IL-1 beta Microplate (12 x 8 wells)", "number":"AB100705-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB100705-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB100705-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-mouse IL-1 beta", "number":"AB100705-CMP10", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Product protocols

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Target data

Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis : its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore.

See full target information Il1b

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Publications (25)

Recent publications for all applications. Explore the full list and refine your search

Marine drugs 21: PubMed37755092

2023

Krill Oil's Protective Benefits against Ultraviolet B-Induced Skin Photoaging in Hairless Mice and In Vitro Experiments.

Applications

Unspecified application

Species

Unspecified reactive species

Jongkyu Kim,Namju Lee,Yoon-Seok Chun,Sang-Hoon Lee,Sae-Kwang Ku

International journal of molecular sciences 23: PubMed36293516

2022

Treadmill Exercise Reduces Neuroinflammation, Glial Cell Activation and Improves Synaptic Transmission in the Prefrontal Cortex in 3 × Tg-AD Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Lianwei Mu,Dongdong Xia,Jiajia Cai,Boya Gu,Xiaojie Liu,Vladislav Friedman,Qing-Song Liu,Li Zhao

Cell biochemistry and biophysics 79:791-799 PubMed34133012

2021

Inhibition of miR-145-5p Reduces Spinal Cord Injury-Induced Inflammatory and Oxidative Stress Responses via Affecting Nurr1-TNF-α Signaling Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Jiang,Zeng-Chun Wei,Li-Li Xu,Shan-Ying Yu,Chao Li

Journal of neuroinflammation 18:119 PubMed34022892

2021

Annexin A1 protects against cerebral ischemia-reperfusion injury by modulating microglia/macrophage polarization via FPR2/ALX-dependent AMPK-mTOR pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Xu,Weiwei Gao,Lei Li,Jiheng Hao,Bin Yang,Tao Wang,Long Li,Xuesong Bai,Fanjian Li,Honglei Ren,Meng Zhang,Liyong Zhang,Jiyue Wang,Dong Wang,Jianning Zhang,Liqun Jiao

Frontiers in pharmacology 12:607403 PubMed33935701

2021

Thunb. Leaves Ameliorate Cognitive Deficit and Neuronal Damage in a Mouse Model of Amyloid-β-Induced Alzheimer's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Eunjin Sohn,Yu Jin Kim,Joo-Hwan Kim,Soo-Jin Jeong

Phytotherapy research : PTR 35:2678-2690 PubMed33432644

2021

Poria cocos could ameliorate cognitive dysfunction in APP/PS1 mice by restoring imbalance of Aβ production and clearance and gut microbiota dysbiosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yufei Sun,Zhiqiang Liu,Zifeng Pi,Fengrui Song,Jianlin Wu,Shu Liu

Frontiers in oncology 10:577280 PubMed33014883

2020

Chitosan-Coated Titanium Dioxide-Embedded Paclitaxel Nanoparticles Enhance Anti-Tumor Efficacy Against Osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Qu,Mingyang Kang,Xueliang Cheng,Jianwu Zhao

Drug design, development and therapy 14:3223-3234 PubMed32848363

2020

Hirudin Protects Against Kidney Damage in Streptozotocin-Induced Diabetic Nephropathy Rats by Inhibiting Inflammation via P38 MAPK/NF-κB Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jiarui Han,Xinxin Pang,Yage Zhang,Zining Peng,Xiujie Shi,Yufeng Xing

Molecular medicine reports 22:2107-2114 PubMed32705172

2020

Vitamin D protects against necrotising enterocolitis in newborn mice by activating the ERK signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chunmei Lyu,Suhua Jiang,Muxian Kong,Xiaoqian Chen,Li Zhang

International journal of molecular medicine 46:239-251 PubMed32377717

2020

Puerarin improves graft bone defect through microRNA‑155‑3p‑mediated p53/TNF‑α/STAT1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Zhou,Hongyu Lian,Kexin Liu,Deli Wang,Xuelian Xiu,Zhang Sun

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse IL-1 beta ELISA Kit

ELISA - Mouse IL-1 beta ELISA Kit (AB100705)

ELISA - Mouse IL-1 beta ELISA Kit (AB100705)

ELISA - Mouse IL-1 beta ELISA Kit (AB100705)

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB100705)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay Platform

Reactivity data

Product details

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (25)

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