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AB108866

Mouse IL-1 beta ELISA kit

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(2 Publications)

Mouse IL-1 beta ELISA kit is a Sandwich (quantitative) ELISA for the measurement of Mouse IL-1 beta in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Interleukin-1 beta, IL-1 beta, Il1b

1 Images
Sandwich ELISA - Mouse IL-1 beta ELISA kit (AB108866)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-1 beta ELISA kit (AB108866)

Representative Standard Curve using ab108866

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 6.7 pg/mL

Range

60 - 500 pg/mL

Assay time

5h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's IL-1 beta (Interleukin-1 beta) mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of mouse IL-1 beta concentrations in plasma, serum and cell culture supernatants.

An IL-1 beta specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an IL-1 beta specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-1 beta captured in plate.

Get better reproducibility in only 90 minutes with Mouse IL-1 beta ELISA Kit (ab197742) from our SimpleStep ELISA® range.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.2" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108866-1x96Tests", "assetComponentDetails": [ { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB108866-CMP05", "productcode":"" }, { "size":"1 x 120 µL", "name":"50X Biotinylated Mouse IL-1 beta Antibody", "number":"AB108866-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"IL-1 beta Standard", "number":"AB108866-CMP07", "productcode":"" }, { "size":"1 x 2 mL", "name":"1X Standard Diluent", "number":"AB108866-CMP02", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB108866-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IL-1 beta Microplate (12 x 8 well strips)", "number":"AB108866-CMP06", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB108866-CMP04", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB108866-CMP10", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB108866-CMP03", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB108866-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis : its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore.
See full target information Il1b
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 10:794198 PubMed35252176

2022

Therapeutic Effects of Kefir Peptides on Hemophilia-Induced Osteoporosis in Mice With Deficient Coagulation Factor VIII.

Applications

Unspecified application

Species

Unspecified reactive species

Chih-Ching Yen,Yao-Wen Liu,Gary Ro-Lin Chang,Ying-Wei Lan,Yung-Tsung Kao,Shin-Nan Cheng,Wei Chen,Chuan-Mu Chen

Nutrition (Burbank, Los Angeles County, Calif.) 31:1430-42 PubMed26429666

2015

Dietary fats significantly influence the survival of penumbral neurons in a rat model of chronic ischemic by modifying lipid mediators, inflammatory biomarkers, NOS production, and redox-dependent apoptotic signals.

Applications

Unspecified application

Species

Unspecified reactive species

Natalia Lausada,Nathalie Arnal,Mariana Astiz,María Cristina Marín,Juan Manuel Lofeudo,Pablo Stringa,María J Tacconi de Alaniz,Nelva Tacconi de Gómez Dumm,Graciela Hurtado de Catalfo,Norma Cristalli de Piñero,María Cristina Pallanza de Stringa,Eva María Illara de Bozzolo,Enrique Gustavo Bozzarello,Diana Olga Cristalli,Carlos Alberto Marra
View all publications
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websiteProtocolBooklet
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Product promise

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