Mouse IL-1 beta ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse IL-1 beta in Cell culture extracts, Cell culture supernatant, Citrate plasma, Serum, Tissue Extracts, Urine samples.
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Key facts
- Detection method
Colorimetric
- Sample types
Cell culture extracts, Cell culture supernatant, Citrate plasma, Serum, Tissue Extracts, Urine
- Assay type
Sandwich (quantitative)
- Reactive species
Mouse
- Range
1.56 - 100 pg/mL
- Assay time
1h 30m
- Sensitivity
= 1 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6.
Alternative names
Interleukin-1 beta, IL-1 beta, Il1b
What's included?
Recommended products
Mouse IL-1 beta ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse IL-1 beta in Cell culture extracts, Cell culture supernatant, Citrate plasma, Serum, Tissue Extracts, Urine samples.
Alternative names
Interleukin-1 beta, IL-1 beta, Il1b
Key facts
- Detection method
Colorimetric
- Sample types
Cell culture extracts, Cell culture supernatant, Citrate plasma, Serum, Tissue Extracts, Urine
- Assay type
Sandwich (quantitative)
- Reactive species
Mouse
- Range
1.56 - 100 pg/mL
- Assay time
1h 30m
- Assay Platform
Microplate (12 x 8 well strips)
- Sensitivity
= 1 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 5 | mean - | SD - | C.V. 6.1 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 3 | mean - | SD - | C.V. 7.7 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 105 | Range 100 - 108 % |
Sample type Serum | Average % = 101 | Range 98 - 103 % |
Sample type Urine | Average % = 94 | Range 91 - 95 % |
Sample type Plasma | Average % = 89 | Range 78 - 95 % |
Sample type Tissue Homogenate | Average % = 97 | Range 95 - 100 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Mouse IL-1 beta ELISA Kit (ab197742) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-1 beta protein in cell culture extracts, cell culture supernatant, cit plasma, serum, tissue extracts, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IL-1 beta with 1 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
ASSAY SPECIFICITY
This kit recognizes both native and recombinant mouse IL-1 beta protein in serum, plasma, and cell culture supernatant, cell and tissue extract samples only.
Mouse brain, kidney, liver and lung homogenates were tested but no signal was detected.
Plasma (EDTA) and plasma (heparin) samples have not been tested with this kit.
CROSS REACTIVITY
Recombinant Human IL-1 beta, Mouse IL-1 alpha, and Mouse IL-1 receptor1 were prepared at 5 ng/mL and assayed for cross reactivity. No cross-reactivity was observed.
INTERFERENCE
Recombinant Human IL-1 beta, Mouse IL-1 alpha, and Mouse IL-1 receptor1 were prepared at 5 ng/mL and tested for interference. No interference with was observed.
SPECIES REACTIVITY
This kit recognizes mouse IL-1 beta protein.
No signal was observed in 25% serum samples from the following species:Human, Dog, Rabbit, Hamster
IL-1 beta (IL-1β) is a 17.5 kDa cytokine protein of the Interleukin 1 family. IL-1β is secreted by macrophages and other cell types in response to inflammatory agents or infection. IL-1β plays a role in a number of cellular processes, including immune responses, bone remodeling, apoptosis and inflammatory pain hypersensitivity.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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15 product images
Sandwich ELISA - Mouse IL-1 beta ELISA Kit (ab197742)
Mouse IL-1 beta standard curve comparison data.
Standard curve comparison between mouse IL-1 beta SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 4-fold increase in sensitivity.
Sandwich ELISA - Mouse IL-1 beta ELISA Kit (ab197742)
Example of IL-1 beta standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse IL-1 beta ELISA Kit (ab197742)
Comparison of secreted IL-1 beta in unstimulated and LPS stimulated RAW264.7 cells.
RAW264.7 cells were grown in the absence (unstimulated) or presence of 5 μg/mL Lipopolysaccharide (LPS) (stimulated) for 48 hours. IL-1 beta was measured in 2-fold diluted cell culture supernatants of unstimulated and LPS stimulated RAW264.7 and cell culture media. Measured values were interpolated from the IL-1 beta Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed: 1.1 pg/mL unstimulated, 83.5 pg/mL stimulated. There was no detectable signal in media.
Sandwich ELISA - Mouse IL-1 beta ELISA Kit (ab197742)
Interpolated concentrations of native IL-1 beta in mouse LPS-stimulated RAW 264.7 extract based on a 50 μg/mL extract load.
The concentrations of IL-1 beta were measured in duplicate and interpolated from the IL-1 beta standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-1 beta concentration was determined to be 70 pg/mL in LPS stimulated RAW 264.7 Lysate.
Sandwich ELISA - Mouse IL-1 beta ELISA Kit (ab197742)
Example of IL-1 beta standard curve in 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse IL-1 beta ELISA Kit (ab197742)
Example of mouse IL-1 beta standard curve in 1X Cell Extraction Buffer PTR.
The IL-1 beta standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse IL-1 beta ELISA Kit (ab197742)
Demonstration of the linearity of dilution by the titration of RAW264.7 stimulated for 48 hours with LPS undiluted to 32-fold dilution in Sample Diluent NS.
Background-subtracted data values (mean +/- SD, n = 2) are graphed.
Sandwich ELISA - Mouse IL-1 beta ELISA Kit (ab197742)
Linearity of dilution in Sample Diluent NS.
Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Native IL-1 beta was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Mouse IL-1 beta ELISA Kit (ab197742)
Example of mouse IL-1 beta standard curve in Sample Diluent NS.
The IL-1 beta standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse IL-1 beta ELISA Kit (ab197742)
Linearity of dilution in 1X Cell Extraction Buffer PTR.
Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Native IL-1 beta was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in 1X Cell Extraction Buffer PTR.
Sandwich ELISA - Mouse IL-1 beta ELISA Kit (ab197742)
Linearity of dilution in Sample Diluent NS (spiked samples).
Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Recombinant IL-1 beta was spiked into the following biological samples and diluted in a 2-fold dilution series in Sample Diluent NS.
25% pooled serum, plasma (Citrate), and urine samples from healthy donors was measured in duplicate. All values were below the detectable range of the assay.
Sandwich ELISA - Mouse IL-1 beta ELISA Kit (ab197742)
Example of mouse IL-1 beta standard curve in Sample Diluent NS in 96-well vs. 384-well plate.
Example of mouse IL-1 beta standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse IL-1 beta ELISA Kit (ab197742)
Interpolated concentrations of mouse IL-1 beta in RAW264.7 cell extract in 96-well vs. 384-well plates.
Interpolated concentration of native IL-1 beta was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1,000 µg/mL RAW264.7 cell extract (1 µg/mL LPS, 48hr). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
Sandwich ELISA - Mouse IL-1 beta ELISA Kit (ab197742)
Example of mouse IL-1 beta standard curve in 1X Cell Extraction Buffer PTR in 96-well vs. 384-well plate.
Example of mouse IL-1 beta standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse IL-1 beta ELISA Kit (ab197742)
Interpolated concentrations of mouse IL-1 beta in stimulated RAW264.7 cell culture supernatant in 96-well vs. 384-well plates.
Interpolated concentration of native IL-1 beta was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 100% stimulated RAW264.7 cell culture supernatant (1 µg/ml LPS, 48hr). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
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