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AB197742

Mouse IL-1 beta ELISA Kit

5

(2 Reviews)

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(204 Publications)

Mouse IL-1 beta ELISA kit is a single-wash, 90-minute SimpleStep ELISA® used to quantify mouse interleukin-1 beta (IL-1β) with a sensitivity of 1 pg/mL. The assay employs a simple Mix-Wash-Read protocol with just one incubation and wash step.

- Colorimetric sanwich ELISA – 450 nm readout, compatible with any standard plate reader
- Available in different formats – 384-well format available for high-throughput applications
- Broad sample compatibility – works with serum, citrate plasma, urine, cell culture extracts, tissue extracts, and cell culture supernatant
- Cited in over 200 publications

View Alternative Names

Interleukin-1 beta, IL-1 beta, Il1b

16 Images
ELISA - Mouse IL-1 beta ELISA Kit (AB197742)
  • ELISA

Supplier Data

ELISA - Mouse IL-1 beta ELISA Kit (AB197742)
Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)

Linearity of dilution in Sample Diluent NS (spiked samples).

Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

Recombinant IL-1 beta was spiked into the following biological samples and diluted in a 2-fold dilution series in Sample Diluent NS.

25% pooled serum, plasma (Citrate), and urine samples from healthy donors was measured in duplicate. All values were below the detectable range of the assay.

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)

Linearity of dilution in Sample Diluent NS.

Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

Native IL-1 beta was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)

Example of mouse IL-1 beta standard curve in 1X Cell Extraction Buffer PTR.

The IL-1 beta standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)

Example of IL-1 beta standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)

Linearity of dilution in 1X Cell Extraction Buffer PTR.

Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

Native IL-1 beta was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)

Demonstration of the linearity of dilution by the titration of RAW264.7 stimulated for 48 hours with LPS undiluted to 32-fold dilution in Sample Diluent NS.

Background-subtracted data values (mean +/- SD, n = 2) are graphed.

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)

Example of IL-1 beta standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)

Interpolated concentrations of native IL-1 beta in mouse LPS-stimulated RAW 264.7 extract based on a 50 μg/mL extract load.

The concentrations of IL-1 beta were measured in duplicate and interpolated from the IL-1 beta standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-1 beta concentration was determined to be 70 pg/mL in LPS stimulated RAW 264.7 Lysate.

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)

Example of mouse IL-1 beta standard curve in Sample Diluent NS.

The IL-1 beta standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)

Comparison of secreted IL-1 beta in unstimulated and LPS stimulated RAW264.7 cells.

RAW264.7 cells were grown in the absence (unstimulated) or presence of 5 μg/mL Lipopolysaccharide (LPS) (stimulated) for 48 hours. IL-1 beta was measured in 2-fold diluted cell culture supernatants of unstimulated and LPS stimulated RAW264.7 and cell culture media. Measured values were interpolated from the IL-1 beta Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed : 1.1 pg/mL unstimulated, 83.5 pg/mL stimulated. There was no detectable signal in media.

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)
  • sELISA

Lab

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)

Mouse IL-1 beta standard curve comparison data.

Standard curve comparison between mouse IL-1 beta SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 4-fold increase in sensitivity.

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)
  • sELISA

Lab

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)

Example of mouse IL-1 beta standard curve in Sample Diluent NS in 96-well vs. 384-well plate.

Example of mouse IL-1 beta standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)
  • sELISA

Lab

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)

Interpolated concentrations of mouse IL-1 beta in RAW264.7 cell extract in 96-well vs. 384-well plates.

Interpolated concentration of native IL-1 beta was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1,000 µg/mL RAW264.7 cell extract (1 µg/mL LPS, 48hr). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)
  • sELISA

Lab

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)

Example of mouse IL-1 beta standard curve in 1X Cell Extraction Buffer PTR in 96-well vs. 384-well plate.

Example of mouse IL-1 beta standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)
  • sELISA

Lab

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)

Interpolated concentrations of mouse IL-1 beta in stimulated RAW264.7 cell culture supernatant in 96-well vs. 384-well plates.

Interpolated concentration of native IL-1 beta was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 100% stimulated RAW264.7 cell culture supernatant (1 µg/ml LPS, 48hr). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Cell culture supernatant, Citrate plasma, Serum, Tissue Extracts, Urine

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 1 pg/mL

Range

1.56 - 100 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse IL-1 beta ELISA Kit ab197742 is a rapid single-wash 90-min ELISA kit to measure cell culture extracts, cell culture supernatant, tissue extracts, urine, serum and plasma. This SimpleStep ELISA® sensitivity is 1 pg/mL.

How the assay works
SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity
This kit recognizes both native and recombinant mouse IL-1 beta protein in serum, plasma, and cell culture supernatant, cell and tissue extract samples only. Mouse brain, kidney, liver and lung homogenates were tested but no signal was detected. Plasma (EDTA) and plasma (heparin) samples have not been tested with this kit.

Mouse IL-1 beta ELISA Kit ab197742 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers are using Mouse IL-1 beta ELISA Kit ab197742
Mouse IL-1 beta ELISA Kit ab197742 has been used to study inflammation in various physio-pathological contexts such as liver disease(1), diabetes(2), and Alzheimer's Disease(3).

References:
(1)Ai-Lei Xu et al. 2024, PMID: 37902450,
(2)Lili Shi et al. 2023, PMID: 38169591,
(3)Guodong Huang, et al. 2023, PMID: 36325883

Save your precious samples
Our 384-well Mouse IL-1 beta ELISA Kit only requires a maxmum of 12.5µL

Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Mouse IL-1beta Antibody Pair - BSA and Azide free ab241673
- Anti-IL-1 beta antibody [EPR16805-1] - BSA and Azide free (Capture) ab242452
- Anti-IL-1 beta antibody [EPR16805-57] - BSA and Azide free (Detector) ab242703

Related and recommended products
Mouse IL-1 β ELISA kit ab197742 is commonly used to measure IL-1 beta as a marker of inflammation.

Other ELISA kits to measure inflammatory markers include:
- Mouse IL-6 ELISA kit ab222503
- Mouse TNF alpha ELISA kit ab208348
- Mouse IL-18 ELISA kit ab216165

IL-1 beta (IL-1β) is a 17.5 kDa cytokine protein of the Interleukin 1 family. IL-1β is secreted by macrophages and other cell types in response to inflammatory agents or infection. IL-1β plays a role in a number of cellular processes, including immune responses, bone remodeling, apoptosis and inflammatory pain hypersensitivity.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.7" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.1" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "100 - 108 %", "average": "= 105" }, { "sample": "Serum", "range": "98 - 103 %", "average": "= 101" }, { "sample": "Urine", "range": "91 - 95 %", "average": "= 94" }, { "sample": "Plasma", "range": "78 - 95 %", "average": "= 89" }, { "sample": "Tissue Homogenate", "range": "95 - 100 %", "average": "= 97" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis : its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore.
See full target information Il1b
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Publications (204)

Recent publications for all applications. Explore the full list and refine your search

Biomolecules & biomedicine 24:505-519 PubMed37902450

2024

Silencing METTL14 alleviates liver injury in non-alcoholic fatty liver disease by regulating mitochondrial homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Wang,Jun Yan,Long Han,Zi-Lin Zou,Ai-Lei Xu

Pharmaceuticals (Basel, Switzerland) 17: PubMed38256924

2024

The Immune-Stimulating and Anti-Diabetic Effects of Leaves Grown in a Plant Factory with Artificial Lights in Immunosuppressed Obese C57BL/6 Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jieun Jung,Ji-Su Kim,Un-Yul Jeong,Ui-Jin Bae,Mina Kim,Shin-Young Park,In-Guk Hwang,Jeong-Wook Heo,Chang-Ki Shim,Jun-Sang Ham,Sung-Hyen Lee

Journal of translational autoimmunity 8:100228 PubMed38225946

2024

ANKRD22 aggravates sepsis-induced ARDS and promotes pulmonary M1 macrophage polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Shi Zhang,Yao Liu,Xiao-Long Zhang,Yun Sun,Zhong-Hua Lu

NPJ systems biology and applications 10:5 PubMed38218856

2024

The antidepressive mechanism of Longya Lilium combined with Fluoxetine in mice with depression-like behaviors.

Applications

Unspecified application

Species

Unspecified reactive species

Huina Ma,Hehua Huang,Chenyu Li,Shasha Li,Juefang Gan,Chunrong Lian,Yanwu Ling

International journal of biological sciences 20:585-605 PubMed38169591

2024

SIRT5-related lysine demalonylation of GSTP1 contributes to cardiomyocyte pyroptosis suppression in diabetic cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Can Wei,Meixin Shi,Shiyun Dong,Zhitao Li,Bingbing Zhao,Dan Liu,Guopeng Li,Jie Cen,Ligen Yu,Xiao Liang,Lili Shi

Microbiology and immunology 68:65-74 PubMed38105559

2023

HPV vaccines induce trained immunity and modulate pro-inflammatory cytokine expression in response to secondary Toll-like receptor stimulations.

Applications

Unspecified application

Species

Unspecified reactive species

Mako Yamaguchi,Yohana S Mtali,Hitomi Sonokawa,Ken Takashima,Yoshimi Fukushima,Takahisa Kouwaki,Hiroyuki Oshiumi

Inflammation research : official journal of the European Histamine Research Society ... [et al.] 73:131-144 PubMed38091015

2023

Homer1 ameliorates ischemic stroke by inhibiting necroptosis-induced neuronal damage and neuroinflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Weihao Lv,Qianqian Zhang,Yuanming Li,Dan Liu,Xiuquan Wu,Xin He,Yuanyuan Han,Xiaowei Fei,Lei Zhang,Zhou Fei

Cellular and molecular life sciences : CMLS 81:4 PubMed38070059

2023

Myeloid SENP3 deficiency protects mice from diet and age-induced obesity via regulation of YAP1 SUMOylation.

Applications

Unspecified application

Species

Unspecified reactive species

Yangjing Jiang,Min Liang,Long Chen,Jian Wang,Yijie Huang,Huanhuan Huo,Danrui Xiao,Yunwen Hu,Zi Wang,Qingqi Ji,Yanjie Li,Zhaohua Cai,Ben He

International journal of molecular sciences 24: PubMed38003466

2023

Prdx6 Regulates Nlrp3 Inflammasome Activation-Driven Inflammatory Response in Lens Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Bhavana Chhunchha,Rakesh Kumar,Eri Kubo,Priyanka Thakur,Dhirendra P Singh

Current issues in molecular biology 45:8882-8893 PubMed37998734

2023

Anti-Allergic Inflammatory Effect of and Its Phlorotannin Component, Trifuhalol A, against the Ovalbumin-Induced Allergic Asthma Model.

Applications

Unspecified application

Species

Unspecified reactive species

Joonki Kim,Sang Heon Lee,Siqi Zhang,Sim-Kyu Bong,Aaron Taehwan Kim,Hara Lee,Xiaoyong Liu,Sang Moo Kim,Su-Nam Kim
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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