Mouse IL-1 beta ELISA kit is a single-wash, 90-minute SimpleStep ELISA® used to quantify mouse interleukin-1 beta (IL-1β) with a sensitivity of 1 pg/mL. The assay employs a simple Mix-Wash-Read protocol with just one incubation and wash step.
- Colorimetric sanwich ELISA – 450 nm readout, compatible with any standard plate reader
- Available in different formats – 384-well format available for high-throughput applications
- Broad sample compatibility – works with serum, citrate plasma, urine, cell culture extracts, tissue extracts, and cell culture supernatant
- Cited in over 200 publications
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore.
Interleukin-1 beta, IL-1 beta, Il1b
Mouse IL-1 beta ELISA kit is a single-wash, 90-minute SimpleStep ELISA® used to quantify mouse interleukin-1 beta (IL-1β) with a sensitivity of 1 pg/mL. The assay employs a simple Mix-Wash-Read protocol with just one incubation and wash step.
- Colorimetric sanwich ELISA – 450 nm readout, compatible with any standard plate reader
- Available in different formats – 384-well format available for high-throughput applications
- Broad sample compatibility – works with serum, citrate plasma, urine, cell culture extracts, tissue extracts, and cell culture supernatant
- Cited in over 200 publications
Sample | n | C.V. |
---|---|---|
Sample Overall | n 5 | C.V. 6.1 |
Sample | n | C.V. |
---|---|---|
Sample Overall | n 3 | C.V. 7.7 |
Sample type | Average % | Range |
---|---|---|
Sample type Cell culture supernatant | Average % = 105 | Range 100 - 108 % |
Sample type Serum | Average % = 101 | Range 98 - 103 % |
Sample type Urine | Average % = 94 | Range 91 - 95 % |
Sample type Plasma | Average % = 89 | Range 78 - 95 % |
Sample type Tissue Homogenate | Average % = 97 | Range 95 - 100 % |
Mouse IL-1 beta ELISA Kit ab197742 is a rapid single-wash 90-min ELISA kit to measure cell culture extracts, cell culture supernatant, tissue extracts, urine, serum and plasma. This SimpleStep ELISA® sensitivity is 1 pg/mL.
How the assay works
SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
This kit recognizes both native and recombinant mouse IL-1 beta protein in serum, plasma, and cell culture supernatant, cell and tissue extract samples only. Mouse brain, kidney, liver and lung homogenates were tested but no signal was detected. Plasma (EDTA) and plasma (heparin) samples have not been tested with this kit.
Mouse IL-1 beta ELISA Kit ab197742 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
How other researchers are using Mouse IL-1 beta ELISA Kit ab197742
Mouse IL-1 beta ELISA Kit ab197742 has been used to study inflammation in various physio-pathological contexts such as liver disease(1), diabetes(2), and Alzheimer's Disease(3).
References:
(1)Ai-Lei Xu et al. 2024, PMID: 37902450,
(2)Lili Shi et al. 2023, PMID: 38169591,
(3)Guodong Huang, et al. 2023, PMID: 36325883
Save your precious samples
Our 384-well Mouse IL-1 beta ELISA Kit only requires a maxmum of 12.5µL
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Mouse IL-1beta Antibody Pair - BSA and Azide free Mouse IL-1beta Antibody Pair - BSA and Azide free ab241673
- Anti-IL-1 beta antibody [EPR16805-1] - BSA and Azide free (Capture) Anti-IL-1 beta antibody [EPR16805-1] - BSA and Azide free (Capture) ab242452
- Anti-IL-1 beta antibody [EPR16805-57] - BSA and Azide free (Detector) Anti-IL-1 beta antibody [EPR16805-57] - BSA and Azide free (Detector) ab242703
Related and recommended products
Mouse IL-1 β ELISA kit ab197742 is commonly used to measure IL-1 beta as a marker of inflammation.
Other ELISA kits to measure inflammatory markers include:
- Mouse IL-6 ELISA kit Mouse IL-6 ELISA Kit ab222503
- Mouse TNF alpha ELISA kit Mouse TNF alpha ELISA Kit ab208348
- Mouse IL-18 ELISA kit Mouse IL-18 ELISA Kit ab216165
IL-1 beta (IL-1β) is a 17.5 kDa cytokine protein of the Interleukin 1 family. IL-1β is secreted by macrophages and other cell types in response to inflammatory agents or infection. IL-1β plays a role in a number of cellular processes, including immune responses, bone remodeling, apoptosis and inflammatory pain hypersensitivity.
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Example of mouse IL-1 beta standard curve in 1X Cell Extraction Buffer PTR in 96-well vs. 384-well plate.
Example of mouse IL-1 beta standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Mouse IL-1 beta standard curve comparison data.
Standard curve comparison between mouse IL-1 beta SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 4-fold increase in sensitivity.
Example of IL-1 beta standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Comparison of secreted IL-1 beta in unstimulated and LPS stimulated RAW264.7 cells.
RAW264.7 cells were grown in the absence (unstimulated) or presence of 5 μg/mL Lipopolysaccharide (LPS) (stimulated) for 48 hours. IL-1 beta was measured in 2-fold diluted cell culture supernatants of unstimulated and LPS stimulated RAW264.7 and cell culture media. Measured values were interpolated from the IL-1 beta Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed: 1.1 pg/mL unstimulated, 83.5 pg/mL stimulated. There was no detectable signal in media.
Interpolated concentrations of native IL-1 beta in mouse LPS-stimulated RAW 264.7 extract based on a 50 μg/mL extract load.
The concentrations of IL-1 beta were measured in duplicate and interpolated from the IL-1 beta standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-1 beta concentration was determined to be 70 pg/mL in LPS stimulated RAW 264.7 Lysate.
Example of IL-1 beta standard curve in 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
Example of mouse IL-1 beta standard curve in 1X Cell Extraction Buffer PTR.
The IL-1 beta standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Demonstration of the linearity of dilution by the titration of RAW264.7 stimulated for 48 hours with LPS undiluted to 32-fold dilution in Sample Diluent NS.
Background-subtracted data values (mean +/- SD, n = 2) are graphed.
Linearity of dilution in Sample Diluent NS.
Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Native IL-1 beta was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.
Example of mouse IL-1 beta standard curve in Sample Diluent NS.
The IL-1 beta standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Linearity of dilution in 1X Cell Extraction Buffer PTR.
Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Native IL-1 beta was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in 1X Cell Extraction Buffer PTR.
Linearity of dilution in Sample Diluent NS (spiked samples).
Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Recombinant IL-1 beta was spiked into the following biological samples and diluted in a 2-fold dilution series in Sample Diluent NS.
25% pooled serum, plasma (Citrate), and urine samples from healthy donors was measured in duplicate. All values were below the detectable range of the assay.
Interpolated concentrations of mouse IL-1 beta in stimulated RAW264.7 cell culture supernatant in 96-well vs. 384-well plates.
Interpolated concentration of native IL-1 beta was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 100% stimulated RAW264.7 cell culture supernatant (1 µg/ml LPS, 48hr). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Example of mouse IL-1 beta standard curve in Sample Diluent NS in 96-well vs. 384-well plate.
Example of mouse IL-1 beta standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Interpolated concentrations of mouse IL-1 beta in RAW264.7 cell extract in 96-well vs. 384-well plates.
Interpolated concentration of native IL-1 beta was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1,000 µg/mL RAW264.7 cell extract (1 µg/mL LPS, 48hr). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
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