AB197742

Mouse IL-1 beta ELISA Kit

Name: Mouse IL-1 beta ELISA Kit (ab197742) | Abcam
Brand: Abcam Limited
SKU: AB197742
Price: 660 USD
Availability: InStock
Rating: 5 (3 reviews)

Recombinant
SimpleStep
What is this?

(3 Reviews)

(275 Publications)

Mouse IL-1 beta ELISA kit is a single-wash, 90-minute SimpleStep ELISA® used to quantify mouse interleukin-1 beta (IL-1β) with a sensitivity of 1 pg/mL. The assay employs a simple Mix-Wash-Read protocol with just one incubation and wash step.

- Colorimetric sanwich ELISA – 450 nm readout, compatible with any standard plate reader
- Available in different formats – 384-well format available for high-throughput applications
- Broad sample compatibility – works with serum, citrate plasma, urine, cell culture extracts, tissue extracts, and cell culture supernatant
- Cited in over 200 publications

View Alternative Names

Interleukin-1 beta, IL-1 beta, Il1b

16 Images

ELISA - Mouse IL-1 beta ELISA Kit (AB197742)

ELISA

Supplier Data

ELISA - Mouse IL-1 beta ELISA Kit (AB197742)

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)

Linearity of dilution in Sample Diluent NS (spiked samples).

Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

Recombinant IL-1 beta was spiked into the following biological samples and diluted in a 2-fold dilution series in Sample Diluent NS.

25% pooled serum, plasma (Citrate), and urine samples from healthy donors was measured in duplicate. All values were below the detectable range of the assay.

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)

Linearity of dilution in Sample Diluent NS.

Native IL-1 beta was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)

Example of mouse IL-1 beta standard curve in 1X Cell Extraction Buffer PTR.

The IL-1 beta standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)

Example of IL-1 beta standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)

Linearity of dilution in 1X Cell Extraction Buffer PTR.

Native IL-1 beta was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)

Demonstration of the linearity of dilution by the titration of RAW264.7 stimulated for 48 hours with LPS undiluted to 32-fold dilution in Sample Diluent NS.

Background-subtracted data values (mean +/- SD, n = 2) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)

Example of IL-1 beta standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)

Interpolated concentrations of native IL-1 beta in mouse LPS-stimulated RAW 264.7 extract based on a 50 μg/mL extract load.

The concentrations of IL-1 beta were measured in duplicate and interpolated from the IL-1 beta standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-1 beta concentration was determined to be 70 pg/mL in LPS stimulated RAW 264.7 Lysate.

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)

Example of mouse IL-1 beta standard curve in Sample Diluent NS.

The IL-1 beta standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)

Comparison of secreted IL-1 beta in unstimulated and LPS stimulated RAW264.7 cells.

RAW264.7 cells were grown in the absence (unstimulated) or presence of 5 μg/mL Lipopolysaccharide (LPS) (stimulated) for 48 hours. IL-1 beta was measured in 2-fold diluted cell culture supernatants of unstimulated and LPS stimulated RAW264.7 and cell culture media. Measured values were interpolated from the IL-1 beta Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed : 1.1 pg/mL unstimulated, 83.5 pg/mL stimulated. There was no detectable signal in media.

sELISA

Lab

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)

Mouse IL-1 beta standard curve comparison data.

Standard curve comparison between mouse IL-1 beta SimpleStep ELISA^® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 4-fold increase in sensitivity.

sELISA

Lab

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)

Example of mouse IL-1 beta standard curve in Sample Diluent NS in 96-well vs. 384-well plate.

Example of mouse IL-1 beta standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)

Interpolated concentrations of mouse IL-1 beta in RAW264.7 cell extract in 96-well vs. 384-well plates.

Interpolated concentration of native IL-1 beta was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1,000 µg/mL RAW264.7 cell extract (1 µg/mL LPS, 48hr). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Lab

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)

Example of mouse IL-1 beta standard curve in 1X Cell Extraction Buffer PTR in 96-well vs. 384-well plate.

Example of mouse IL-1 beta standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)

Interpolated concentrations of mouse IL-1 beta in stimulated RAW264.7 cell culture supernatant in 96-well vs. 384-well plates.

Interpolated concentration of native IL-1 beta was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 100% stimulated RAW264.7 cell culture supernatant (1 µg/ml LPS, 48hr). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Cell culture supernatant, Citrate plasma, Serum, Tissue Extracts, Urine

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1 pg/mL

Range

1.56 - 100 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

Mouse IL-1 beta ELISA Kit ab197742 is a rapid single-wash 90-min ELISA kit to measure cell culture extracts, cell culture supernatant, tissue extracts, urine, serum and plasma. This SimpleStep ELISA® sensitivity is 1 pg/mL.

How the assay works
SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity
This kit recognizes both native and recombinant mouse IL-1 beta protein in serum, plasma, and cell culture supernatant, cell and tissue extract samples only. Mouse brain, kidney, liver and lung homogenates were tested but no signal was detected. Plasma (EDTA) and plasma (heparin) samples have not been tested with this kit.

Mouse IL-1 beta ELISA Kit ab197742 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers are using Mouse IL-1 beta ELISA Kit ab197742
Mouse IL-1 beta ELISA Kit ab197742 has been used to study inflammation in various physio-pathological contexts such as liver disease⁽¹⁾, diabetes⁽²⁾, and Alzheimer's Disease⁽³⁾.

References:
⁽¹⁾Ai-Lei Xu et al. 2024, PMID: 37902450,
⁽²⁾Lili Shi et al. 2023, PMID: 38169591,
⁽³⁾Guodong Huang, et al. 2023, PMID: 36325883

Save your precious samples
Our 384-well Mouse IL-1 beta ELISA Kit only requires a maxmum of 12.5µL

Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Mouse IL-1beta Antibody Pair - BSA and Azide free ab241673
- Anti-IL-1 beta antibody [EPR16805-1] - BSA and Azide free (Capture) ab242452
- Anti-IL-1 beta antibody [EPR16805-57] - BSA and Azide free (Detector) ab242703

Related and recommended products
Mouse IL-1 β ELISA kit ab197742 is commonly used to measure IL-1 beta as a marker of inflammation.

Other ELISA kits to measure inflammatory markers include:
- Mouse IL-6 ELISA kit ab222503
- Mouse TNF alpha ELISA kit ab208348
- Mouse IL-18 ELISA kit ab216165

IL-1 beta (IL-1β) is a 17.5 kDa cytokine protein of the Interleukin 1 family. IL-1β is secreted by macrophages and other cell types in response to inflammatory agents or infection. IL-1β plays a role in a number of cellular processes, including immune responses, bone remodeling, apoptosis and inflammatory pain hypersensitivity.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style

left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.7" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.1" } ]

style

left-column

Recovery

[ { "sample": "Cell culture supernatant", "range": "100 - 108 %", "average": "= 105" }, { "sample": "Serum", "range": "98 - 103 %", "average": "= 101" }, { "sample": "Urine", "range": "91 - 95 %", "average": "= 94" }, { "sample": "Plasma", "range": "78 - 95 %", "average": "= 89" }, { "sample": "Tissue Homogenate", "range": "95 - 100 %", "average": "= 97" } ]

style

left-column

What's included?

{ "values": { "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab197742-1x384Tests", "assetComponentDetails": [ { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB197742-CMP05" }, { "size":"6 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB197742-CMP07" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB197742-CMP12" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB197742-CMP06" }, { "size":"2 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB197742-CMP03" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB197742-CMP13" }, { "size":"1 x 600 µL", "name":"10X Mouse IL-1 beta Capture Antibody", "number":"AB197742-CMP09" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB197742-CMP11" }, { "size":"1 x 50 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB197742-CMP08" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB197742-CMP01" }, { "size":"1 x 600 µL", "name":"10X Mouse IL-1 beta Detector Antibody", "number":"AB197742-CMP10" }, { "size":"2 x 1 Vial", "name":"Mouse IL-1 beta Lyophilized Recombinant Protein", "number":"AB197742-CMP02" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab197742-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB197742-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB197742-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB197742-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB197742-CMP07", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB197742-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB197742-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB197742-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse IL-1 beta Capture Antibody", "number":"AB197742-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse IL-1 beta Lyophilized Recombinant Protein", "number":"AB197742-CMP02", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB197742-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse IL-1 beta Detector Antibody", "number":"AB197742-CMP10", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB197742-CMP08", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

style

left-column

style

left-column

Product protocols

style

left-column

Target data

Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis : its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore.

See full target information Il1b

style

left-column

Publications (275)

Recent publications for all applications. Explore the full list and refine your search

Journal of pharmaceutical analysis 15:101251 PubMed40521370

2025

Impact of FASN-enriched EVs on endothelial cell function in obstructive sleep apnea hypopnea syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Tian,Dan Zhang,Huaian Yang,Xiaoli Zhang,Shengqun Xu

Naunyn-Schmiedeberg's archives of pharmacology : PubMed40514518

2025

Alpha-asarone relieves nasal inflammation, epithelial barrier damage, and mitochondrial damage in allergic rhinitis by inhibiting mitochondrial ROS via the SIRT1/PGC-1α pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Xu,Weimin Gao,Haitong Li,Xiaojuan Cao,Xiaohong Chen,Nannan Dong,Lingfang Wu,Yunzhen Luo

Journal of cell communication and signaling 19:e70021 PubMed40486531

2025

Gut microbiota-derived trimethylamine-N-oxide inhibits SIRT1 to regulate SM22α-mediated smooth muscle cell inflammation and promote atherosclerosis progression.

Applications

Unspecified application

Species

Unspecified reactive species

Yajuan Yin,Mei Wei,Xiufang Jiang,Mei Liu,Xiaocui Shi,Xiao Zhang,Le Wang,Gang Liu,Mingqi Zheng,Fangfang Ma

Neuromolecular medicine 27:34 PubMed40374872

2025

Far-Infrared Radiation Ameliorates the Cognitive Dysfunction in an Alzheimer's Disease Transgenic Mouse via Modulating Jak-2/Stat3 and Nrf-2/HO-1 Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Wen Yang,Qiuxia Yu,Nick Wang,Koon Kit Lam,Zhi-Xiu Lin,Yan-Fang Xian

Frontiers in immunology 16:1557235 PubMed40313946

2025

roots extract to rescue coccidiosis-mediated inflammation in the jejunum of C57BL/6 mice.

Applications

Unspecified application

Species

Unspecified reactive species

Rewaida Abdel-Gaber,Ghada Alamari,Mohamed A Dkhil,Saeed El-Ashram,Nawal Al-Hoshani,Esam M Al-Shaebi,Saleh Al-Quraishy

Genes 16: PubMed40282416

2025

IL-6 Affects Liver Metabolic Abnormalities Caused by Silicon Exposure by Regulating the PKC/YY1 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Zhao,Huihui Tao,Jian Gao,Jingjing Wang,Guangliang Hui,Ye Zhu,Jialin Wang,Xuansheng Ding,Yong Dai

Science advances 11:eadt6113 PubMed40249812

2025

Progesterone signaling in oviductal epithelial cells modulates the immune response to support preimplantation embryonic development.

Applications

Unspecified application

Species

Unspecified reactive species

Emily A McGlade,Jiude Mao,Kalli K Stephens,Ryan M Marquardt,Feyza Nur Arguc,Peter F Lais,San-Pin Wu,Sarayut Winuthayanon,Haval Shirwan,Esma S Yolcu,Mark I Hunter,James K Pru,John P Lydon,Francesco J DeMayo,Wipawee Winuthayanon

Communications biology 8:457 PubMed40102597

2025

Peli1, regulated by mA modification, suppresses NLRP3 inflammasome activation in atherosclerosis by inhibiting YB-1.

Applications

Unspecified application

Species

Unspecified reactive species

Qiang Liu,Lu Yan,Tao Wu,Qinghua Wu,Ben Ke,Wen Shen

Frontiers in immunology 16:1558983 PubMed40093002

2025

Distinct microbial signatures and their predictive value in recurrent acute pancreatitis: insights from 5-region 16S rRNA gene sequencing.

Applications

Unspecified application

Species

Unspecified reactive species

Qi-Wen Wang,Haorui Zheng,Yang Yang,Xinyao Chang,Zengkan Du,Zi-Ning Hang,Zhao-Shen Li,Zhuan Liao

BMC neuroscience 26:21 PubMed40055623

2025

Esketamine attenuates traumatic brain injury by modulating STAT3-mediated Glycolysis and immune responses.

Applications

Unspecified application

Species

Unspecified reactive species

Yufang Liu,Zheng Gong,Longfei Zhang,Xian Yang,Jie Zhu,Xin Zhou,Xingzhi Liao

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse IL-1 beta ELISA Kit

ELISA - Mouse IL-1 beta ELISA Kit (AB197742)

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)

Sandwich ELISA - Mouse IL-1 beta ELISA Kit (AB197742)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Assay Kits

Secondary Antibodies

Assay Kits

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (275)

Product promise