Mouse IL-1 beta ELISA Kit, Fluorescent is a single-wash 90-min Simplestep used to quantify Mouse IL-1 beta with a sensitivity of 0.2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Fluorescent Sandwich ELISA - 530/570/590 nm readout : works on any standard plate reader
- Cited in over 5 citations
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- Journal of neuroinflammation 20:1292023The neuroprotective N-terminal amyloid-β core hexapeptide reverses reactive gliosis and gliotoxicity in Alzheimer's disease pathology models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMegan J Lantz,Alyssa M Roberts,Donovan D Delgado,Robert A NicholsPubMed 37245024
- Translational neurodegeneration 12:102023Inflammasome activation under high cholesterol load triggers a protective microglial phenotype while promoting neuronal pyroptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCristina de Dios,Xenia Abadin,Vicente Roca-Agujetas,Marina Jimenez-Martinez,Albert Morales,Ramon Trullas,Montserrat Mari,Anna ColellPubMed 36895045
- Cardiovascular research 119:969-9812022Cholesterol accumulation in macrophages drives NETosis in atherosclerotic plaques via IL-1β secretion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMustafa Yalcinkaya,Panagiotis Fotakis,Wenli Liu,Kaori Endo-Umeda,Huijuan Dou,Sandra Abramowicz,Tong Xiao,Peter Libby,Nan Wang,Alan R Tall,Marit WesterterpPubMed 36537208
- Advanced therapeutics 5:2022A single-dose Qβ VLP vaccine against S100A9 protein reduces atherosclerosis in a preclinical model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOscar A Ortega-Rivera,Matthew D Shin,Miguel A Moreno-Gonzalez,Jonathan K Pokorski,Nicole F SteinmetzPubMed 36570039
- International journal of molecular sciences 21:2020Behaviour of Vascular Smooth Muscle Cells on Amine Plasma-Coated Materials with Various Chemical Structures and Morphologies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIvana Nemcakova et. al.PubMed 33322781
Key facts
- Detection method
- Fluorescent
- Sample types
- Cell culture extracts, Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum, Tissue Extracts, Urine
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 0.2 - 200 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 0.2 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore.
Alternative names
Interleukin-1 beta, IL-1 beta, Il1b
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Mouse IL-1 beta ELISA Kit, Fluorescent is a single-wash 90-min Simplestep used to quantify Mouse IL-1 beta with a sensitivity of 0.2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Fluorescent Sandwich ELISA - 530/570/590 nm readout : works on any standard plate reader
- Cited in over 5 citations
Key facts
- Detection method
- Fluorescent
- Sample types
- Cell culture extracts, Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum, Tissue Extracts, Urine
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 0.2 - 200 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 0.2 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Cell Media | n 5 | C.V. 6.1 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Cell Media | n 3 | C.V. 7.7 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 105 | Range 100 - 108 % |
Sample type Serum | Average % = 101 | Range 98 - 103 % |
Sample type Urine | Average % = 94 | Range 91 - 95 % |
Sample type Plasma | Average % = 89 | Range 78 - 95 % |
Sample type Tissue Homogenate | Average % = 97 | Range 95 - 100 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
IL-1 beta (Interleukin-1 beta) in vitro CatchPoint SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-1 beta (Interleukin-1 beta) protein in mouse serum, plasma, urine, cell culture supernatant, and cell and tissue extracts.
This CatchPoint SimpleStep ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
If using a Molecular Devices' plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org.
The CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.
Interleukin-1 beta (IL-1 beta) is a 17.5 kDa cytokine protein of the Interleukin 1 family. IL-1 beta is secreted by macrophages and other cell types in response to inflammatory agents or infection. IL-1 beta plays a role in a number of cellular processes, including immune responses, bone remodeling, apoptosis and inflammatory pain hypersensitivity.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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3 product images
Sandwich ELISA - Mouse IL-1 beta ELISA Kit, Fluorescent (ab229440)
Example of mouse IL-1 beta (Interleukin-1 beta) standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse IL-1 beta ELISA Kit, Fluorescent (ab229440)
Comparison of secreted mouse IL-1 beta in unstimulated and LPS stimulated RAW264.7 cells.
RAW264.7 cells were grown in the absence (unstimulated) or presence of 5 μg/mL Lipopolysaccharide (LPS) (stimulated) for 48 hours. IL-1 beta was measured in 2-fold diluted cell culture supernatants of unstimulated and LPS stimulated RAW264.7 and cell culture media. Measured values were interpolated from the IL-1 beta Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed: 1.1 pg/mL unstimulated, 83.5 pg/mL stimulated. There was no detectable signal in media.
Sandwich ELISA - Mouse IL-1 beta ELISA Kit, Fluorescent (ab229440)
Demonstration of the linearity of dilution by the titration of RAW264.7 stimulated for 48 hours with LPS undiluted to 32-fold dilution in Sample Diluent NS. Background-subtracted data values (mean +/- SD, n = 2) are graphed.
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