AB229440

Mouse IL-1 beta ELISA Kit, Fluorescent

Recombinant
CatchPoint SimpleStep ELISA
What is this?

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(6 Publications)

Mouse IL-1 beta ELISA Kit, Fluorescent is a single-wash 90-min Simplestep used to quantify Mouse IL-1 beta with a sensitivity of 0.2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Fluorescent Sandwich ELISA - 530/570/590 nm readout : works on any standard plate reader
- Cited in over 5 citations

View Alternative Names

Interleukin-1 beta, IL-1 beta, Il1b

3 Images

Sandwich ELISA - Mouse IL-1 beta ELISA Kit, Fluorescent (AB229440)

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-1 beta ELISA Kit, Fluorescent (AB229440)

Example of mouse IL-1 beta (Interleukin-1 beta) standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-1 beta ELISA Kit, Fluorescent (AB229440)

Comparison of secreted mouse IL-1 beta in unstimulated and LPS stimulated RAW264.7 cells.

RAW264.7 cells were grown in the absence (unstimulated) or presence of 5 μg/mL Lipopolysaccharide (LPS) (stimulated) for 48 hours. IL-1 beta was measured in 2-fold diluted cell culture supernatants of unstimulated and LPS stimulated RAW264.7 and cell culture media. Measured values were interpolated from the IL-1 beta Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed : 1.1 pg/mL unstimulated, 83.5 pg/mL stimulated. There was no detectable signal in media.

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-1 beta ELISA Kit, Fluorescent (AB229440)

Demonstration of the linearity of dilution by the titration of RAW264.7 stimulated for 48 hours with LPS undiluted to 32-fold dilution in Sample Diluent NS. Background-subtracted data values (mean +/- SD, n = 2) are graphed.

Key facts

Detection method

Fluorescent

Sample types

Cell culture extracts, Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum, Tissue Extracts, Urine

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.2 pg/mL

Range

0.2 - 200 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

IL-1 beta (Interleukin-1 beta) in vitro CatchPoint SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-1 beta (Interleukin-1 beta) protein in mouse serum, plasma, urine, cell culture supernatant, and cell and tissue extracts.

This CatchPoint SimpleStep ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
If using a Molecular Devices' plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org.

The CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.

Interleukin-1 beta (IL-1 beta) is a 17.5 kDa cytokine protein of the Interleukin 1 family. IL-1 beta is secreted by macrophages and other cell types in response to inflammatory agents or infection. IL-1 beta plays a role in a number of cellular processes, including immune responses, bone remodeling, apoptosis and inflammatory pain hypersensitivity.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Cell Media", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.7" }, { "reproducibilityType": "Intra", "sample": "Cell Media", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.1" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "100 - 108 %", "average": "= 105" }, { "sample": "Serum", "range": "98 - 103 %", "average": "= 101" }, { "sample": "Urine", "range": "91 - 95 %", "average": "= 94" }, { "sample": "Plasma", "range": "78 - 95 %", "average": "= 89" }, { "sample": "Tissue Homogenate", "range": "95 - 100 %", "average": "= 97" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab229440-1x96Tests", "assetComponentDetails": [ { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB229440-CMP13", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB229440-CMP08", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB229440-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB229440-CMP12", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse IL-1 beta Capture Antibody", "number":"AB229440-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse IL-1 beta Lyophilized Recombinant Protein", "number":"AB229440-CMP03", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB229440-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB229440-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stoplight Red Substrate Buffer", "number":"AB229440-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse IL-1 beta Detector Antibody", "number":"AB229440-CMP11", "productcode":"" }, { "size":"1 x 50 µL", "name":"500X Hydrogen Peroxide (H2O2, 3%)", "number":"AB229440-CMP07", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB229440-CMP09", "productcode":"" }, { "size":"1 x 120 µL", "name":"100X Stoplight Red Substrate", "number":"AB229440-CMP01", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis : its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore.

See full target information Il1b

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Nature cardiovascular research 3:60-75 PubMed38362011

2024

Suppression of IL-1β promotes beneficial accumulation of fibroblast-like cells in atherosclerotic plaques in clonal hematopoiesis.

Applications

Unspecified application

Species

Unspecified reactive species

Trevor P Fidler,Andrew Dunbar,Eunyoung Kim,Brian Hardaway,Jessica Pauli,Chenyi Xue,Sandra Abramowicz,Tong Xiao,Kavi O'Connor,Nadja Sachs,Nan Wang,Lars Maegdefessel,Ross Levine,Muredach Reilly,Alan R Tall

Journal of neuroinflammation 20:129 PubMed37245024

2023

The neuroprotective N-terminal amyloid-β core hexapeptide reverses reactive gliosis and gliotoxicity in Alzheimer's disease pathology models.

Applications

Unspecified application

Species

Unspecified reactive species

Megan J Lantz,Alyssa M Roberts,Donovan D Delgado,Robert A Nichols

Translational neurodegeneration 12:10 PubMed36895045

2023

Inflammasome activation under high cholesterol load triggers a protective microglial phenotype while promoting neuronal pyroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Cristina de Dios,Xenia Abadin,Vicente Roca-Agujetas,Marina Jimenez-Martinez,Albert Morales,Ramon Trullas,Montserrat Mari,Anna Colell

Cardiovascular research 119:969-981 PubMed36537208

2022

Cholesterol accumulation in macrophages drives NETosis in atherosclerotic plaques via IL-1β secretion.

Applications

Unspecified application

Species

Unspecified reactive species

Mustafa Yalcinkaya,Panagiotis Fotakis,Wenli Liu,Kaori Endo-Umeda,Huijuan Dou,Sandra Abramowicz,Tong Xiao,Peter Libby,Nan Wang,Alan R Tall,Marit Westerterp

Advanced therapeutics 5: PubMed36570039

2022

A single-dose Qβ VLP vaccine against S100A9 protein reduces atherosclerosis in a preclinical model.

Applications

Unspecified application

Species

Unspecified reactive species

Oscar A Ortega-Rivera,Matthew D Shin,Miguel A Moreno-Gonzalez,Jonathan K Pokorski,Nicole F Steinmetz

International journal of molecular sciences 21: PubMed33322781

2020

Behaviour of Vascular Smooth Muscle Cells on Amine Plasma-Coated Materials with Various Chemical Structures and Morphologies.

Applications

Unspecified application

Species

Unspecified reactive species

Ivana Nemcakova,Lucie Blahova,Petr Rysanek,Andreu Blanquer,Lucie Bacakova,Lenka Zajíčková

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse IL-1 beta ELISA Kit, Fluorescent

Sandwich ELISA - Mouse IL-1 beta ELISA Kit, Fluorescent (AB229440)

Sandwich ELISA - Mouse IL-1 beta ELISA Kit, Fluorescent (AB229440)

Sandwich ELISA - Mouse IL-1 beta ELISA Kit, Fluorescent (AB229440)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Assay Kits

Secondary Antibodies

Assay Kits

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (6)

Product promise