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AB213871

Mouse IL-1 RL1 ELISA Kit (ST2)

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(1 Publication)

Mouse IL-1 RL1 ELISA Kit (ST2) is a Sandwich (quantitative) ELISA for the measurement of Mouse IL-1 RL1 (ST2) in Mouse in Cell/Tissue Extracts, Cell Culture Media, Biofluids samples.

View Alternative Names

Ly84, St2, Ste2, Il1rl1, Interleukin-1 receptor-like 1, Interleukin-33 receptor alpha chain, Lymphocyte antigen 84, Protein ST2, Protein T1

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Sandwich ELISA - Mouse IL-1 RL1 ELISA Kit (ST2) (AB213871)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-1 RL1 ELISA Kit (ST2) (AB213871)

Mouse IL1RL1 ELISA Kit (ab213871) Standard Curve.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

< 10 pg/mL

Range

156 - 10000 pg/mL

Assay time

3h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The Mouse IL1RL1 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213871) is designed for the quantitative measurement of Mouse IL1RL1 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL1RL1 has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: S27-R332) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL1RL1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse IL1RL1 amount of sample captured in plate.

Interleukin 1 receptor-like 1, also known as IL1RL1 or ST2, is a protein that in humans is encoded by the IL1RL1 gene. The protein encoded by this gene is a member of the interleukin 1 receptor family. Studies of the similar gene in mouse suggested that this receptor can be induced by proinflammatory stimuli, and may be involved in the function of helper T cells. IL1RL1 is necessary for endotoxin tolerance and, by inhibiting TLR responses, enhances Th2 responses. This gene, interleukin 1 receptor, type I (IL1R1), interleukin 1 receptor, type II (IL1R2) and interleukin 1 receptor-like 2 (IL1RL2) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "189 pg/mL", "standardDeviation": "10.77", "coefficientOfVariability": "5.7" }, { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "1538 pg/mL", "standardDeviation": "115.35", "coefficientOfVariability": "7.5" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "5883 pg/mL", "standardDeviation": "394.16", "coefficientOfVariability": "6.7" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "194 pg/mL", "standardDeviation": "8.73", "coefficientOfVariability": "4.5" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "1525 pg/mL", "standardDeviation": "94.55", "coefficientOfVariability": "6.2" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "5484 pg/mL", "standardDeviation": "296.13", "coefficientOfVariability": "5.4" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab213871-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Antibody Diluent Buffer", "number":"AB213871-CMP07", "productcode":"" }, { "size":"1 x 100 µL", "name":"Biotinylated anti- Mouse IL1RL1 antibody", "number":"AB213871-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Stop Solution", "number":"AB213871-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized recombinant Mouse IL1RL1 standard", "number":"AB213871-CMP01", "productcode":"" }, { "size":"4 x 1 Unit", "name":"Adhesive Plate Seal", "number":"AB213871-CMP06", "productcode":"" }, { "size":"1 x 30 mL", "name":"Sample Diluent Buffer", "number":"AB213871-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Color Developing Agent", "number":"AB213871-CMP04", "productcode":"" }, { "size":"1 x 100 µL", "name":"Avidin-Biotin-Peroxidase Complex (ABC)", "number":"AB213871-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Mouse IL1RL1 coated Microplate (12 x 8 wells)", "number":"AB213871-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"Wash Buffer (25X)", "number":"AB213871-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"ABC Diluent Buffer", "number":"AB213871-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ST2 also known as IL-1RL1 or IL-1RL1 chimera protein is a member of the IL-1 receptor family. It has a molecular mass of approximately 55 kDa. This protein exists in different forms including a membrane-bound form and a soluble variant. Researchers typically find ST2 expressed in various tissues including the lungs gut and cardiovascular system. The expression level can vary significantly depending on the physiological or pathological state.
Biological function summary

ST2 interacts with interleukin-33 (IL-33) and functions as its receptor. The binding of IL-33 to ST2 activates immune responses particularly influencing T-helper 2 (Th2) cells. ST2 forms part of a receptor complex including IL-1 receptor accessory protein IL-1RAcP necessary for signal transduction. This interaction leads to the activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) and mitogen-activated protein kinase (MAPK) signaling pathways facilitating inflammatory responses.

Pathways

IL-33 signals through the ST2 receptor participating actively in the immune signaling pathway. This pathway plays a significant role in modulating inflammatory and immune responses. ST2 association with NF-kB broadly affects inflammatory signaling while its involvement with the MAPK pathway aids in cellular responses to cytokines and stress. The close relationship with the IL-33/ST2 axis highlights its importance in maintaining immune homeostasis and reacting to environmental stressors.

ST2 has significant associations with asthma and cardiovascular diseases. In asthma the IL-33/ST2 axis critically contributes to airway inflammation and hyperresponsiveness. Similarly in the context of cardiovascular diseases elevated levels of soluble ST2 are markers of heart failure with the ST2/IL-33 axis contributing to cardiac remodeling. ST2's connection with inflammatory processes implies it also interacts with other cytokines such as interleukin-6 (IL-6) exacerbating the disease states through shared pathways.
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Product protocols

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Target data

Receptor for interleukin-33 (IL-33) which plays crucial roles in innate and adaptive immunity, contributing to tissue homeostasis and responses to environmental stresses together with coreceptor IL1RAP (PubMed : 17675517, PubMed : 18450470, PubMed : 22660580, PubMed : 29045903). Its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8 (By similarity). Possibly involved in helper T-cell function (By similarity). Upon tissue injury, induces UCP2-dependent mitochondrial rewiring that attenuates the generation of reactive oxygen species and preserves the integrity of Krebs cycle required for persistent production of itaconate and subsequent GATA3-dependent differentiation of inflammation-resolving alternatively activated macrophages (PubMed : 34644537).. Isoform B. Inhibits IL-33 signaling.
See full target information Il1rl1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Inflammation research : official journal of the European Histamine Research Society ... [et al.] 70:569-579 PubMed33852061

2021

Molecular hydrogen alleviates asthma through inhibiting IL-33/ILC2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jingxi Zhang,Xiumin Feng,Yunxin Fan,Guanglin Zhu,Chong Bai
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