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Mouse IL-10 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Mouse IL-10 in Mouse in Plasma, Cell culture supernatant, Serum samples.
Alternative names=Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, Il10, Il-10
- Detection method
Colorimetric
- Sample types
Plasma, Cell culture supernatant, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Mouse
- Range
78.13 - 5000 pg/mL
- Sensitivity
< 45 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Mouse IL-10 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Mouse IL-10 in Mouse in Plasma, Cell culture supernatant, Serum samples.
Alternative names=Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, Il10, Il-10
Key facts
- Detection method
Colorimetric
- Sample types
Plasma, Cell culture supernatant, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Mouse
- Range
78.13 - 5000 pg/mL
- Assay Platform
Microplate
- Sensitivity
< 45 pg/mL
- General recovery
93%
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 103.7 | Range 79 - 120 % |
Sample type Serum | Average % = 85.97 | Range 77 - 93 % |
Sample type Plasma | Average % = 92.2 | Range 71 - 134 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Abcam's Mouse IL-10 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of IL-10 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse IL-10 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-10 antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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