AB100697

Mouse IL-10 ELISA Kit

Name: Mouse IL-10 ELISA Kit (ab100697) | Abcam
Brand: Abcam Limited
SKU: AB100697
Price: 780 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(25 Publications)

Mouse IL-10 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse IL-10 in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Il-10, Il10, Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF

2 Images

Sandwich ELISA - Mouse IL-10 ELISA Kit (AB100697)

sELISA

Unknown

Sandwich ELISA - Mouse IL-10 ELISA Kit (AB100697)

Representative standard curve using ab100697

sELISA

Unknown

Sandwich ELISA - Mouse IL-10 ELISA Kit (AB100697)

Representative standard curve using ab100697

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

< 45 pg/mL

Range

78.13 - 5000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Abcam's Mouse IL-10 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of IL-10 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for mouse IL-10 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-10 antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "79 - 120 %", "average": "= 103.7" }, { "sample": "Serum", "range": "77 - 93 %", "average": "= 85.97" }, { "sample": "Plasma", "range": "71 - 134 %", "average": "= 92.2" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100697-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100697-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100697-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D", "number":"AB100697-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB100697-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100697-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Mouse IL-10", "number":"AB100697-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100697-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse IL-10 Standard (lyophilized)", "number":"AB100697-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IL-10 Microplate (12 x 8 wells)", "number":"AB100697-CMP06", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Product protocols

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Target data

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (By similarity). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity) (PubMed : 28473584).

See full target information Il10

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Publications (25)

Recent publications for all applications. Explore the full list and refine your search

Molecular neurodegeneration 19:80 PubMed39456006

2024

Repetitive transcranial magnetic stimulation alleviates motor impairment in Parkinson's disease: association with peripheral inflammatory regulatory T-cells and SYT6.

Applications

Unspecified application

Species

Unspecified reactive species

Fen Xie,Bibiao Shen,Yuqi Luo,Hang Zhou,Zhenchao Xie,Shuzhen Zhu,Xiaobo Wei,Zihan Chang,Zhaohua Zhu,Changhai Ding,Kunlin Jin,Chengwu Yang,Lucia Batzu,K Ray Chaudhuri,Ling-Ling Chan,Eng-King Tan,Qing Wang

Antioxidants (Basel, Switzerland) 13: PubMed38539873

2024

Potential Phytotherapy of DSS-Induced Colitis: Ameliorating Reactive Oxygen Species-Mediated Necroptosis and Gut Dysbiosis with a New Bunge Variety-Daehong.

Applications

Unspecified application

Species

Unspecified reactive species

Kang-In Lee,Yousang Jo,Heung Joo Yuk,Sun-Young Kim,Hyungjun Kim,Hye Jin Kim,Soo-Keol Hwang,Ki-Sun Park

International journal of molecular sciences 24: PubMed37373162

2023

Synaptamide Ameliorates Hippocampal Neurodegeneration and Glial Activation in Mice with Traumatic Brain Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Tyrtyshnaia,Olga Manzhulo,Igor Manzhulo

International journal of biological sciences 19:1163-1177 PubMed36923935

2023

Lipocalin-2 promotes acute lung inflammation and oxidative stress by enhancing macrophage iron accumulation.

Applications

Unspecified application

Species

Unspecified reactive species

Hyeong Seok An,Jung-Wan Yoo,Jong Hwan Jeong,Manbong Heo,Si Hwan Hwang,Hye Min Jang,Eun Ae Jeong,Jaewoong Lee,Hyun Joo Shin,Kyung Eun Kim,Meong Cheol Shin,Gu Seob Roh

Cells 11: PubMed36230938

2022

DICAM in the Extracellular Vesicles from Astrocytes Attenuates Microglia Activation and Neuroinflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Han,Hyun-Jung Cho,Donghwi Park,Seungwoo Han

Animals : an open access journal from MDPI 12: PubMed36077992

2022

Effects of Para-Toluenesulfonamide on Canine Melanoma Xenotransplants in a BALB/c Nude Mouse Model.

Applications

Unspecified application

Species

Unspecified reactive species

Chien-Teng Lin,Chuen-Fu Lin,Jui-Te Wu,Hsiao-Pei Tsai,Shu-Ying Cheng,Huei-Jyuan Liao,Tzu-Chun Lin,Chao-Hsuan Wu,Yu-Chin Lin,Jiann-Hsiung Wang,Geng-Ruei Chang

Frontiers in pharmacology 13:904317 PubMed35721103

2022

Beneficial Effect of Edoxaban on Preventing Atrial Fibrillation and Coagulation by Reducing Inflammation HBG1/HBD Biomarkers.

Applications

Unspecified application

Species

Unspecified reactive species

Chenguang Yang,Xiang Wang,Ying Guo,Xuyang Meng,Yi Li,Chenxi Xia,Lingbing Meng,Min Dong,Fang Wang

Journal of parasitic diseases : official organ of the Indian Society for Parasitology 46:491-501 PubMed35692479

2022

Mitigation of -induced ileitis by infection pinpointing immunomodulation.

Applications

Unspecified application

Species

Unspecified reactive species

Enas F Abdel Hamed,Nahed E Mostafa,Mohsen M Hassan,Mohamed N Ibrahim,Said Mahmoud Elraey,Mona Radwan,Al-Sayed R Al-Attar,Eman M Fawzy

International journal of molecular sciences 22: PubMed34639071

2021

Anti-Inflammatory Activity of -Docosahexaenoylethanolamine and -Eicosapentaenoylethanolamine in a Mouse Model of Lipopolysaccharide-Induced Neuroinflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Tyrtyshnaia,Sophia Konovalova,Anatoly Bondar,Ekaterina Ermolenko,Ruslan Sultanov,Igor Manzhulo

Allergologia et immunopathologia 49:72-77 PubMed34476925

2021

Coniferyl aldehyde alleviates LPS-induced WI-38 cell apoptosis and inflammation injury via JAK2-STAT1 pathway in acute pneumonia.

Applications

Unspecified application

Species

Unspecified reactive species

Yichun He,Qin Li,Weijun Zhou,Yanhong Gu,Yu Jiang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse IL-10 ELISA Kit

Sandwich ELISA - Mouse IL-10 ELISA Kit (AB100697)

Sandwich ELISA - Mouse IL-10 ELISA Kit (AB100697)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay Platform

Reactivity data

Product details

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (25)

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Product promise