AB108870

Mouse IL-10 ELISA kit

Name: Mouse IL-10 ELISA kit (ab108870) | Abcam
Brand: Abcam Limited
SKU: AB108870
Price: 780 USD
Availability: InStock
Rating: 3 (1 reviews)

(1 Review)

(40 Publications)

Mouse IL-10 ELISA kit is a Sandwich (quantitative) ELISA for the measurement of Mouse IL-10 in Mouse in Biofluids, Cell Culture Media, Cell/Tissue Extracts samples.

View Alternative Names

Il-10, Il10, Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF

1 Images

Sandwich ELISA - Mouse IL-10 ELISA kit (AB108870)

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-10 ELISA kit (AB108870)

Representative Standard Curve using ab108870

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum, Cell Lysate, Tissue

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 15 pg/mL

Range

31.25 - 2000 pg/mL

Assay time

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Abcam's IL-10 Mouse in vitro ELISA (enzyme-linked immunosorbent assay) kit is designed for the quantitative measurement of IL-10 in plasma, serum, tissue extracts, cell lysates and cell culture supernatants.

An IL-10 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an IL-10 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-10 captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.9" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108870-1x96Tests", "assetComponentDetails": [ { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB108870-CMP04", "productcode":"" }, { "size":"1 x 120 µL", "name":"50X Biotinylated Mouse IL-10 Antibody", "number":"AB108870-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IL-10 Microplate (12 x 8 well strips)", "number":"AB108870-CMP05", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB108870-CMP03", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB108870-CMP01", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB108870-CMP07", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB108870-CMP09", "productcode":"" }, { "size":"1 x 2 mL", "name":"1X Standard Diluent", "number":"AB108870-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"IL-10 Standard", "number":"AB108870-CMP06", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB108870-CMP08", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

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Product protocols

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Target data

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (By similarity). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity) (PubMed : 28473584).

See full target information Il10

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Publications (40)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 28:e70228 PubMed39622780

2024

Geraniin Alleviates Mouse Laser-Induced Choroidal Neovascularisation by Inhibiting Choroidal Endothelial Cell ACE2/Ang-(1-7)/MasR/IL-10 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyi Lu,Qi Cai,Lele Li,Jiayi Gu,Yuting Zhang,Haotian Sun,Hui Su,Lei Song

Marine drugs 21: PubMed37755092

2023

Krill Oil's Protective Benefits against Ultraviolet B-Induced Skin Photoaging in Hairless Mice and In Vitro Experiments.

Applications

Unspecified application

Species

Unspecified reactive species

Jongkyu Kim,Namju Lee,Yoon-Seok Chun,Sang-Hoon Lee,Sae-Kwang Ku

American journal of physiology. Heart and circulatory physiology 325:H77-H88 PubMed37145957

2023

Sex hormones impact early maturation and immune response in the arteriovenous fistula mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Keyuree Satam,Yuichi Ohashi,Carly Thaxton,Luis Gonzalez,Ocean Setia,Hualong Bai,Yukihiko Aoyagi,Yangzhouyun Xie,Weichang Zhang,Bogdan Yatsula,Kathleen A Martin,Yujun Cai,Alan Dardik

Folia neuropathologica 60:76-91 PubMed35359148

2022

Inhibition of poly (ADP-ribose) polymerase 1 in neuron cells might enhance the therapeutic effect of leonurine under cholesterol stimulation.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Hou,Zhigang Huan,Chao Zhang,Zhiyong Chen,Lingmei Ha,Shengxuan Huang

Bioengineered 13:2927-2942 PubMed35038955

2022

MicroRNA-665-3p exacerbates nonalcoholic fatty liver disease in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanjie Yu,Tian Tian,Shiyun Tan,Pengbo Wu,Yitian Guo,Ming Li,Mengjun Huang

Analytical cellular pathology (Amsterdam) 2021:4853355 PubMed35004133

2021

MicroRNA-137-3p Improves Nonalcoholic Fatty Liver Disease through Activating AMPK.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanjie Yu,Chunping He,Shiyun Tan,Mengjun Huang,Yitian Guo,Ming Li,Qian Zhang

Frontiers in cellular neuroscience 15:765541 PubMed35002625

2021

Interaction of α9α10 Nicotinic Receptors With Peptides and Proteins From Animal Venoms.

Applications

Unspecified application

Species

Unspecified reactive species

Victor Tsetlin,Yves Haufe,Valentina Safronova,Dmitriy Serov,PranavKumar Shadamarshan,Lina Son,Irina Shelukhina,Denis Kudryavtsev,Elena Kryukova,Igor Kasheverov,Annette Nicke,Yuri Utkin

Bioengineered 12:11707-11715 PubMed34783296

2021

Arbutin ameliorated ulcerative colitis of mice induced by dextran sodium sulfate (DSS).

Applications

Unspecified application

Species

Unspecified reactive species

Chuan Zhang,Huiping Zhu,Hui Jie,Hengyue Ding,Hongwen Sun

Open medicine (Warsaw, Poland) 16:1090-1100 PubMed34414282

2021

Long noncoding RNA XIST knockdown relieves the injury of microglia cells after spinal cord injury by sponging miR-219-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Xueren Zhong,Yongzheng Bao,Qiang Wu,Xinhua Xi,Wengang Zhu,Sanmei Chen,Junjian Liao

Journal of translational medicine 19:264 PubMed34140005

2021

Long non-coding RNA MEG3 mediates the miR-149-3p/FOXP3 axis by reducing p53 ubiquitination to exert a suppressive effect on regulatory T cell differentiation and immune escape in esophageal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Qi-Rong Xu,Jian Tang,Hong-Ying Liao,Ben-Tong Yu,Xiang-Yuan He,Yu-Zhen Zheng,Sheng Liu

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse IL-10 ELISA kit

Sandwich ELISA - Mouse IL-10 ELISA kit (AB108870)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (40)

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Product promise