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Mouse IL-10 ELISA kit is a Sandwich (quantitative) ELISA kit for the measurement of Mouse IL-10 in Mouse in Plasma, Cell culture supernatant, Serum, Cell Lysate, Tissue samples.


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Sandwich ELISA - Mouse IL-10 ELISA kit (AB108870), expandable thumbnail

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Key facts

Detection method
Colorimetric
Sample types
Plasma, Cell culture supernatant, Serum, Cell Lysate, Tissue
Assay type
Sandwich (quantitative)
Reactive species
Mouse
Range
0.125 - 1 ng/mL
Assay time
5h
Sensitivity
= 0.014 ng/mL

Reactivity data

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

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Select an associated product type

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Target data

Function

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (By similarity). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity) (PubMed:28473584).

Alternative names

Il-10, Il10, Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF

What's included?

1 x 96 Tests
Components
100X Streptavidin-Peroxidase Conjugate
1 x 80 µL
10X Diluent N Concentrate
1 x 30 mL
1X Standard Diluent
1 x 2 mL
20X Wash Buffer Concentrate
2 x 30 mL
50X Biotinylated Mouse IL-10 Antibody
1 x 120 µL
Chromogen Substrate
1 x 7 mL
IL-10 Microplate (12 x 8 well strips)
1 x 1 Unit
IL-10 Standard
1 x 1 Vial
Sealing Tapes
3 x 1 Unit
Stop Solution
1 x 11 mL

Recommended products

Mouse IL-10 ELISA kit is a Sandwich (quantitative) ELISA kit for the measurement of Mouse IL-10 in Mouse in Plasma, Cell culture supernatant, Serum, Cell Lysate, Tissue samples.

Key facts

Detection method
Colorimetric
Sample types
Plasma, Cell culture supernatant, Serum, Cell Lysate, Tissue
Assay type
Sandwich (quantitative)
Reactive species
Mouse
Range
0.125 - 1 ng/mL
Assay time
5h
Assay Platform
Microplate
Sensitivity
= 0.014 ng/mL
General recovery
97%

Precision

Intra assay

Sample
Overall
n
0
mean
-
SD
-
C.V.
6

Inter assay

Sample
Overall
n
0
mean
-
SD
-
C.V.
10.9

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols

Notes

Abcam's IL-10 Mouse in vitro ELISA (enzyme-linked immunosorbent assay) kit is designed for the quantitative measurement of IL-10 in plasma, serum, tissue extracts and cell culture supernatants.

An IL-10 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an IL-10 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-10 captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Sandwich ELISA - Mouse IL-10 ELISA kit (ab108870), expandable thumbnail

    Sandwich ELISA - Mouse IL-10 ELISA kit (ab108870)

    Representative Standard Curve using ab108870

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com