AB46103

Mouse IL-10 ELISA Kit

Name: Mouse IL-10 ELISA Kit (ab46103) | Abcam
Brand: Abcam Limited
SKU: AB46103
Price: 760 USD
Availability: InStock

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(13 Publications)

Mouse IL-10 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse IL-10 in Mouse in Cell Culture Media, Biofluids samples.

View Alternative Names

Il-10, Il10, Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF

1 Images

Sandwich ELISA - Mouse IL-10 ELISA Kit (AB46103)

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-10 ELISA Kit (AB46103)

Representative Standard Curve using ab46103

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

< 20 pg/mL

Range

31.25 - 1000 pg/mL

Assay time

3h 45m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Abcam's IL-10 mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-10 in mouse serum, buffered solutions and cell culture medium.

A monoclonal antibody specific for IL-10 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-10 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-10 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-10 present in the samples.

This kit will recognize both endogenous and recombinant mouse IL-10.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample A", "replicates": 8, "mean": "548.2 pg/mL", "standardDeviation": "12.1", "coefficientOfVariability": "2.2" }, { "reproducibilityType": "Inter", "sample": "Sample B", "replicates": 8, "mean": "133.6 pg/mL", "standardDeviation": "5.4", "coefficientOfVariability": "4" }, { "reproducibilityType": "Intra", "sample": "Sample A", "replicates": 8, "mean": "556.8 pg/mL", "standardDeviation": "11.6", "coefficientOfVariability": "2" }, { "reproducibilityType": "Intra", "sample": "Sample B", "replicates": 8, "mean": "137.4 pg/mL", "standardDeviation": "6.8", "coefficientOfVariability": "4.9" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab46103-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"HRP Diluent", "number":"AB46103-CMP04" }, { "size":"2 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46103-CMP06" }, { "size":"1 x 400 µL", "name":"Biotinylated anti-IL-10", "number":"AB46103-CMP01" }, { "size":"1 x 7.5 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46103-CMP02" }, { "size":"1 x 1 Unit", "name":"IL-10 Microplate (12 x 8 well strips)", "number":"AB46103-CMP10" }, { "size":"1 x 10 mL", "name":"200X Wash Buffer", "number":"AB46103-CMP07" }, { "size":"1 x 15 mL", "name":"10X Standard Diluent Buffer", "number":"AB46103-CMP08" }, { "size":"1 x 11 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46103-CMP09" }, { "size":"2 x 1 Vial", "name":"Murine IL-10 standard", "number":"AB46103-CMP05" }, { "size":"1 x 11 mL", "name":"Stop Reagent", "number":"AB46103-CMP03" } ] }, "2x96Tests": { "sellingSize": "2 x 96 Tests", "publicAssetCode":"ab46103-2x96Tests", "assetComponentDetails": [ { "size":"1 x 23 mL", "name":"HRP Diluent", "number":"AB46103-CMP04", "productcode":"" }, { "size":"1 x 13 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46103-CMP02", "productcode":"" }, { "size":"2 x 400 µL", "name":"Biotinylated anti-IL-10", "number":"AB46103-CMP01", "productcode":"" }, { "size":"2 x 10 mL", "name":"200X Wash Buffer", "number":"AB46103-CMP07", "productcode":"" }, { "size":"2 x 1 Unit", "name":"IL-10 Microplate (12 x 8 well strips)", "number":"AB46103-CMP10", "productcode":"" }, { "size":"1 x 24 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46103-CMP09", "productcode":"" }, { "size":"4 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46103-CMP06", "productcode":"" }, { "size":"1 x 25 mL", "name":"10X Standard Diluent Buffer", "number":"AB46103-CMP08", "productcode":"" }, { "size":"4 x 1 Vial", "name":"Murine IL-10 standard", "number":"AB46103-CMP05", "productcode":"" }, { "size":"2 x 11 mL", "name":"Stop Reagent", "number":"AB46103-CMP03", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (By similarity). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity) (PubMed : 28473584).

See full target information Il10

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

EBioMedicine 90:104480 PubMed36863256

2023

A small-molecule inhibitor of Keap1-Nrf2 interaction attenuates sepsis by selectively augmenting the antibacterial defence of macrophages at infection sites.

Applications

Unspecified application

Species

Unspecified reactive species

Yawei Wang,Binlin Tang,Huijuan Li,Jiancheng Zheng,Can Zhang,Zeyu Yang,Xu Tan,Peng Luo,Le Ma,Yang Wang,Lei Long,Zelin Chen,Zhenliang Xiao,Lijie Ma,Jing Zhou,Yu Wang,Chunmeng Shi

Journal of neuroinflammation 19:122 PubMed35624490

2022

Intermittent fasting reduces neuroinflammation in intracerebral hemorrhage through the Sirt3/Nrf2/HO-1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shuhui Dai,Jialiang Wei,Hongchen Zhang,Peng Luo,Yuefan Yang,Xiaofan Jiang,Zhou Fei,Wenbin Liang,Jianli Jiang,Xia Li

Cell death discovery 8:260 PubMed35568721

2022

microRNA-27a-3p delivered by extracellular vesicles from glioblastoma cells induces M2 macrophage polarization via the EZH1/KDM3A/CTGF axis.

Applications

Unspecified application

Species

Unspecified reactive species

Guifang Zhao,Hongquan Yu,Lijuan Ding,Weiyao Wang,Huan Wang,Yao Hu,Lingsha Qin,Guangce Deng,Buqing Xie,Guofeng Li,Ling Qi

International journal of molecular sciences 20: PubMed31557796

2019

Mesenchymal Stem Cells Extract (MSCsE)-Based Therapy Alleviates Xerostomia and Keratoconjunctivitis Sicca in Sjogren's Syndrome-Like Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Ghada Abughanam,Osama A Elkashty,Younan Liu,Mohammed O Bakkar,Simon D Tran

International immunopharmacology 76:105835 PubMed31476692

2019

MicroRNA-124 alleviates the lung injury in mice with septic shock through inhibiting the activation of the MAPK signaling pathway by downregulating MAPK14.

Applications

Unspecified application

Species

Unspecified reactive species

Weiyun Pan,Na Wei,Weiling Xu,Gang Wang,Fangchao Gong,Na Li

Journal of neuroinflammation 16:91 PubMed30995916

2019

Role of NADPH oxidase-2 in the progression of the inflammatory response secondary to striatum excitotoxic damage.

Applications

Unspecified application

Species

Unspecified reactive species

Diego Rolando Hernández-Espinosa,Lourdes Massieu,Teresa Montiel,Julio Morán

Journal of immunology (Baltimore, Md. : 1950) 201:3199-3210 PubMed30373853

2018

FcγRIIb on B Cells and Myeloid Cells Modulates B Cell Activation and Autoantibody Responses via Different but Synergistic Pathways in Lupus-Prone Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Qingshun Lin,Mareki Ohtsuji,Hirofumi Amano,Hiromichi Tsurui,Norihiro Tada,Ryota Sato,Hidehiro Fukuyama,Hiroyuki Nishimura,J Sjef Verbeek,Sachiko Hirose

Probiotics and antimicrobial proteins 11:857-864 PubMed29682678

2018

More Protection of Lactobacillus acidophilus Than Bifidobacterium bifidum Probiotics on Azoxymethane-Induced Mouse Colon Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shahram Agah,Ali Mohammad Alizadeh,Maryam Mosavi,Peyman Ranji,Hossein Khavari-Daneshvar,Farnaz Ghasemian,Sahar Bahmani,Abbas Tavassoli

Frontiers in pharmacology 9:347 PubMed29695964

2018

Root Extract of Ameliorates DSS-Induced Ulcerative Colitis by Affecting NF-kappaB Signaling Pathway in a Mouse Model via Synergistic Effects of Polydatin, Resveratrol, and Emodin.

Applications

Unspecified application

Species

Unspecified reactive species

Baohai Liu,Shuangdi Li,Xiaodan Sui,Lianyi Guo,Xingmei Liu,Hongmei Li,Leming Gao,Shusheng Cai,Yanrong Li,Tingting Wang,Xuehua Piao

Frontiers in cellular and infection microbiology 7:157 PubMed28536674

2017

Anti-Idiotypic Antibodies Specific to prM Monoantibody Prevent Antibody Dependent Enhancement of Dengue Virus Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Miao Wang,Fan Yang,Dana Huang,Yalan Huang,Xiaomin Zhang,Chao Wang,Shaohua Zhang,Renli Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse IL-10 ELISA Kit

Sandwich ELISA - Mouse IL-10 ELISA Kit (AB46103)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (13)

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Product promise