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AB47599

Mouse IL-10 ELISA Set (without plates)

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Mouse IL-10 ELISA Set (without plates) is a Sandwich (quantitative) ELISA for the measurement of Mouse IL-10 ELISA Set (without plates) in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Il-10, Il10, Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF

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Sandwich ELISA - Mouse IL-10 ELISA Set (without plates) (AB47599)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-10 ELISA Set (without plates) (AB47599)

Murine IL-10 standard curve ranging from 31.25 to 1000 pg/mL

Key facts

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

< 20 pg/mL

Range

31.25 - 1000 pg/mL

Assay time

3h 45m

Assay Platform

Reagents

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's IL-10 Murine in vitro ELISA Set is designed for the quantitative measurement of Murine IL-10 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.

The murine IL-10 ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.

This ELISA Set is intended to measure murine IL-10 in cell -culture supernatants, as well as in serum samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.

Recognizes both natural and recombinant Murine IL-10

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (By similarity). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity) (PubMed : 28473584).
See full target information Il10
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websiteProtocolBooklet
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