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AB100698

Mouse IL-11 ELISA Kit

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(1 Publication)

Mouse IL-11 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse IL-11 in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Interleukin-11, IL-11, Il11, IL 11, Interleukin 11

1 Images
ELISA - Mouse IL-11 ELISA Kit (AB100698)
  • ELISA

Supplier Data

ELISA - Mouse IL-11 ELISA Kit (AB100698)

Representative standard curve using ab100698.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

< 1 ng/mL

Range

1.85 - 450 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's IL-11 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-11 in plasma and cell culture supernatants. (Mouse IL-11 concentration is pretty low in normal plasma, it may not be detected in this assay).

This assay employs an antibody specific for mouse IL-11 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-11 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-11 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-11 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

We have not been able to detect the endogenous Mouse IL-11 in normal serum with ab100698, only in serum spiked with Mouse IL-11.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "70 - 87 %", "average": "= 77.35" }, { "sample": "Plasma", "range": "95 - 113 %", "average": "= 117.3" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100698-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100698-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100698-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse IL-11 Standard (lyophilized)", "number":"AB100698-CMP07", "productcode":"" }, { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin Concentrate", "number":"AB100698-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-mouse IL-11", "number":"AB100698-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IL-11 Microplate (12 x 8 wells)", "number":"AB100698-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100698-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D", "number":"AB100698-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100698-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Cytokine that stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production (PubMed : 8913282). Also promotes the proliferation of hepatocytes in response to liver damage (PubMed : 22253262). Binding to its receptor formed by IL6ST and either IL11RA1 or IL11RA2 activates a signaling cascade that promotes cell proliferation, also in the context of various cancers (PubMed : 10026196, PubMed : 23948300). Signaling leads to the activation of intracellular protein kinases and the phosphorylation of STAT3 (PubMed : 22253262, PubMed : 23948300). The interaction with the membrane-bound IL11RA and IL6ST stimulates 'classic signaling', whereas the binding of IL11 and soluble IL11RA to IL6ST stimulates 'trans-signaling' (By similarity).
See full target information Il11
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

American journal of physiology. Gastrointestinal and liver physiology 316:G251-G262 PubMed30520693

2018

Overexpression of IL-11 promotes premalignant gastric epithelial hyperplasia in isolation from germline gp130-JAK-STAT driver mutations.

Applications

Unspecified application

Species

Unspecified reactive species

Jon N Buzzelli,Louise O'Connor,Michelle Scurr,Sharleen Chung Nien Chin,Angelique Catubig,Garrett Z Ng,Masanobu Oshima,Hiroko Oshima,Andrew S Giraud,Philip Sutton,Louise M Judd,Trevelyan R Menheniott
View all publications
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websiteProtocolBooklet
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