AB215084

Mouse IL-11 ELISA Kit

Recombinant
SimpleStep
What is this?

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(2 Publications)

Mouse IL-11 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse IL-11 with a sensitivity of 17.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Interleukin-11, IL-11, Il11, IL 11, Interleukin 11

5 Images

ELISA - Mouse IL-11 ELISA Kit (AB215084)

ELISA

Supplier Data

ELISA - Mouse IL-11 ELISA Kit (AB215084)

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-11 ELISA Kit (AB215084)

Mouse lung cells were cultured unstimulated or stimulated with 10 ng/mL TPA and 1 ng/mL IL-1a.

Conditioned media was harvested after 48 hours. IL-11 was measured in 100% unstimulated and stimulated lung supernatant. The concentrations of IL-11 were measured in duplicate, interpolated from the IL-11 standard curves. The interpolated values are plotted (mean +/- SD, n=2). The mean IL-11 concentration was determined to be 1849 pg/mL in TPA and IL-1a stimulated lung supernatant and 809 pg/mL in unstimulated lung supernatant.

sELISA

Lab

Sandwich ELISA - Mouse IL-11 ELISA Kit (AB215084)

Example of mouse IL-11 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-11 ELISA Kit (AB215084)

Interpolated concentrations of native IL-11 in mouse lung cell culture supernatant samples.

The concentrations of IL-11 were measured in duplicates and interpolated from the IL-11 standard curves. Undiluted samples are as follows : lung supernatant stimulated with TPA and IL-1a 100%. The interpolated values are plotted (mean +/- SD, n=2). The mean IL-11 concentration was determined to be 2143 pg/mL in lung supernatant stimulated with TPA and IL-1a.

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-11 ELISA Kit (AB215084)

Example of mouse IL-11 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 17.4 pg/mL

Range

78.12 - 5000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse IL-11 ELISA Kit ab215084 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse IL-11 in cell culture supernatant, citrate plasma, serum. This SimpleStep sensitivity is 17.4 pg/mL.

How the assay works

Mouse IL-11 SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse IL-11 ELISA Kit ab215084 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Save your precious samples

Our 384-well Mouse IL-11 ELISA Kit only requires a maximum of 12.5µL sample

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-IL-11 antibody [EPR19660-167] - BSA and Azide free (Capture) ab242849
- Anti-IL-11 antibody [EPR19660-171] - BSA and Azide free (Detector) ab242849

IL-11 is a multifunctional cytokine that is a key regulator of hematopoiesis. It stimulates hematopoietic stem cell and megakaryocyte progenitor cell proliferation. It is also involved in the response to liver damage by inducing proliferation of hepatocytes.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.3" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.4" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "98 - 105 %", "average": "= 102" }, { "sample": "Serum", "range": "98 - 101 %", "average": "= 100" }, { "sample": "Citrate plasma", "range": "86 - 97 %", "average": "= 91" } ]

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What's included?

{ "values": { "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab215084-1x384Tests", "assetComponentDetails": [ { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB215084-CMP07" }, { "size":"1 x 600 µL", "name":"10X Mouse IL-11 Capture Antibody", "number":"AB215084-CMP02" }, { "size":"2 x 1 Vial", "name":"Mouse IL-11 Lyophilized Recombinant Protein", "number":"AB215084-CMP04" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB215084-CMP05" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB215084-CMP12" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB215084-CMP10" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB215084-CMP08" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB215084-CMP11" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB215084-CMP09" }, { "size":"1 x 600 µL", "name":"10X Mouse IL-11 Detector Antibody", "number":"AB215084-CMP03" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab215084-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB215084-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse IL-11 Lyophilized Recombinant Protein", "number":"AB215084-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB215084-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB215084-CMP12", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB215084-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse IL-11 Capture Antibody", "number":"AB215084-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB215084-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse IL-11 Detector Antibody", "number":"AB215084-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB215084-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB215084-CMP06", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Cytokine that stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production (PubMed : 8913282). Also promotes the proliferation of hepatocytes in response to liver damage (PubMed : 22253262). Binding to its receptor formed by IL6ST and either IL11RA1 or IL11RA2 activates a signaling cascade that promotes cell proliferation, also in the context of various cancers (PubMed : 10026196, PubMed : 23948300). Signaling leads to the activation of intracellular protein kinases and the phosphorylation of STAT3 (PubMed : 22253262, PubMed : 23948300). The interaction with the membrane-bound IL11RA and IL6ST stimulates 'classic signaling', whereas the binding of IL11 and soluble IL11RA to IL6ST stimulates 'trans-signaling' (By similarity).

See full target information Il11

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

mAbs 14:2104153 PubMed35916739

2022

Development of novel ultra-sensitive IL-11 target engagement assays to support mechanistic PK/PD modeling for an anti-IL-11 antibody therapeutic.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Myzithras,Siqi Lin,Legairre Radden,Cynthia Hess Kenny,Zheng Cai,Angus MacDonald,Ralph Binetti,Michael Marlow,Paul Fracasso,Glenn Gibson,Christina Bartlett,Julie Hawkins,Steven Hansel

Scientific reports 11:6838 PubMed33767224

2021

Inhibition of ERK 1/2 kinases prevents tendon matrix breakdown.

Applications

Unspecified application

Species

Unspecified reactive species

Ulrich Blache,Stefania L Wunderli,Amro A Hussien,Tino Stauber,Gabriel Flückiger,Maja Bollhalder,Barbara Niederöst,Sandro F Fucentese,Jess G Snedeker

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse IL-11 ELISA Kit

ELISA - Mouse IL-11 ELISA Kit (AB215084)

Sandwich ELISA - Mouse IL-11 ELISA Kit (AB215084)

Sandwich ELISA - Mouse IL-11 ELISA Kit (AB215084)

Sandwich ELISA - Mouse IL-11 ELISA Kit (AB215084)

Sandwich ELISA - Mouse IL-11 ELISA Kit (AB215084)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (2)

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