AB236717

Mouse IL-12 p40 ELISA Kit

Recombinant
SimpleStep
What is this?

Be the first to review this product! Submit a review

(7 Publications)

Mouse IL-12 p40 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse IL-12 p40 with a sensitivity of 3.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations

View Alternative Names

Interleukin-12 subunit beta, IL-12B, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL-12 subunit p40, CLMF p40, Il12b

3 Images

Sandwich ELISA - Mouse IL-12 p40 ELISA Kit (AB236717)

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-12 p40 ELISA Kit (AB236717)

Interpolated concentrations of native IL-12 p40 in mouse serum and plasma samples.

The concentrations of IL-12 p40 were measured in duplicates, interpolated from the IL-12 p40 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-12 p40 concentration was determined to be 1509 pg/mL in serum, 856 pg/mL in plasma (citrate), 822 pg/mL in plasma (EDTA) and 2285 pg/mL in plasma (heparin).

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-12 p40 ELISA Kit (AB236717)

Interpolated concentrations of spiked IL-12 p40 in mouse cell culture media samples.

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-12 p40 ELISA Kit (AB236717)

Example of mouse IL-12 p40 standard curve in Sample Diluent NS.

The IL-12 p40 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich

Sensitivity

= 3.7 pg/mL

Range

15.6 - 1000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

Mouse IL-12 p40 ELISA Kit (ab236717) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-12 p40 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IL-12 p40 with 3.7 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

style

left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.1" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.5" } ]

style

left-column

Recovery

[ { "sample": "Serum", "range": "108 - 124 %", "average": "= 115" }, { "sample": "EDTA Plasma", "range": "118 - 125 %", "average": "= 122" }, { "sample": "Heparin Plasma", "range": "103 - 113 %", "average": "= 107" }, { "sample": "Citrate plasma", "range": "105 - 114 %", "average": "= 111" }, { "sample": "Cell culture media", "range": "92 - 96 %", "average": "= 94" } ]

style

left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab236717-1x96Tests", "assetComponentDetails": [ { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB236717-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB236717-CMP07", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB236717-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB236717-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB236717-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse IL-12 p40 Capture Antibody", "number":"AB236717-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse IL-12 p40 Lyophilized Recombinant Protein", "number":"AB236717-CMP04", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB236717-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse IL-12 p40 Detector Antibody", "number":"AB236717-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB236717-CMP06", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

style

left-column

style

left-column

Product protocols

style

left-column

Target data

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.. Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of pro-inflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.

See full target information Il12b

style

left-column

Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Journal of orthopaedic surgery and research 19:517 PubMed39198853

2024

Novel insights into vancomycin-loaded calcium sulfate and negative pressure wound therapy in preventing infections in open fractures.

Applications

Unspecified application

Species

Unspecified reactive species

Bei Jia,Rui Xue,Jia Li,Jichao Guo,Jianning Liu

Molecules (Basel, Switzerland) 29: PubMed38675716

2024

Beneficial Effects of Dietary Fiber in Young Barley Leaf on Gut Microbiota and Immunity in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Seita Chudan,Takuto Kurakawa,Miyu Nishikawa,Yoshinori Nagai,Yoshiaki Tabuchi,Shinichi Ikushiro,Yukihiro Furusawa

Journal of functional biomaterials 14: PubMed36976084

2023

Ketoprofen-Based Polymer-Drug Nanoparticles Provide Anti-Inflammatory Properties to HA/Collagen Hydrogels.

Applications

Unspecified application

Species

Unspecified reactive species

Norbert Halfter,Eva Espinosa-Cano,Gloria María Pontes-Quero,Rosa Ana Ramírez-Jiménez,Christiane Heinemann,Stephanie Möller,Matthias Schnabelrauch,Hans-Peter Wiesmann,Vera Hintze,Maria Rosa Aguilar

Frontiers in pharmacology 12:627716 PubMed33995024

2021

Xuebijing Protects Against Septic Acute Liver Injury Based on Regulation of GSK-3β Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Liping Cao,Zhenghong Li,Yi Ren,Mengmeng Wang,Zhizhou Yang,Wei Zhang,Xiaoqin Han,Mengya Yao,Zhaorui Sun,Shinan Nie

Journal of neuroinflammation 17:187 PubMed32539736

2020

Orexin A alleviates neuroinflammation via OXR2/CaMKKβ/AMPK signaling pathway after ICH in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Li,Weilin Xu,Jinsong Ouyang,Xiaoyang Lu,Prativa Sherchan,Cameron Lenahan,Giselle Irio,John H Zhang,Jianhua Zhao,Yongfa Zhang,Jiping Tang

International journal of molecular medicine 44:324-334 PubMed31059072

2019

Total flavone of Abelmoschus manihot ameliorates Crohn's disease by regulating the NF‑κB and MAPK signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Zhang,Ping Zhu,Yue Liu,Yi Shu,Jin-Yong Zhou,Feng Jiang,Tuo Chen,Bo-Lin Yang,Yu-Gen Chen

Veterinary research 50:31 PubMed31046828

2019

Acetate metabolic requirement of avian pathogenic Escherichia coli promotes its intracellular proliferation within macrophage.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangkai Zhuge,Yu Sun,Min Jiang,Juanfang Wang,Fang Tang,Feng Xue,Jianluan Ren,Weiyun Zhu,Jianjun Dai

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse IL-12 p40 ELISA Kit

Sandwich ELISA - Mouse IL-12 p40 ELISA Kit (AB236717)

Sandwich ELISA - Mouse IL-12 p40 ELISA Kit (AB236717)

Sandwich ELISA - Mouse IL-12 p40 ELISA Kit (AB236717)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (7)

Complementary Products

Assay Kits

Assay Kits

Assay Kits

Assay Kits

Product promise