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AB100699

Mouse IL-12 p40 + IL-12 p70 ELISA Kit

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(4 Publications)

Mouse IL-12 p40 + IL-12 p70 ELISA Kit is a Sandwich ELISA for the measurement of Mouse IL-12 p40 + IL-12 p70 in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Interleukin-12 subunit alpha, IL-12A, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12 subunit p35, CLMF p35, Il12a, Interleukin-12 subunit beta, IL-12B, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL-12 subunit p40, CLMF p40, Il12b

2 Images
Sandwich ELISA - Mouse IL-12 p40 + IL-12 p70 ELISA Kit (AB100699)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-12 p40 + IL-12 p70 ELISA Kit (AB100699)

Representative standard curve using ab100699

Sandwich ELISA - Mouse IL-12 p40 + IL-12 p70 ELISA Kit (AB100699)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-12 p40 + IL-12 p70 ELISA Kit (AB100699)

Representative standard curve using ab100699

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 5 pg/mL

Range

2.74 - 2000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's IL-12 p40 + IL-12 p70 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IL-12 p40 + IL-12 p70 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Mouse IL-12 p40 + IL-12 p70 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-12 p40 + IL-12 p70 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-IL-12 p40 + IL-12 p70 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

The standard is affinity purified.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "84 - 103 %", "average": "= 95.25" }, { "sample": "Serum", "range": "82 - 102 %", "average": "= 93.29" }, { "sample": "Plasma", "range": "83 - 103 %", "average": "= 94.52" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100699-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100699-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100699-CMP07", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100699-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin Concentrate", "number":"AB100699-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IL-12 p40/p70 Microplate (12 x 8 wells)", "number":"AB100699-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse IL-12 p40/p70 Standard (lyophilized)", "number":"AB100699-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Mouse IL-12 p40/p70", "number":"AB100699-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100699-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100699-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Heterodimerizes with IL12B to form the IL-12 cytokine or with EBI3/IL27B to form the IL-35 cytokine. IL-12 is primarily produced by professional antigen-presenting cells (APCs) such as B-cells and dendritic cells (DCs) as well as macrophages and granulocytes and regulates T-cell and natural killer-cell responses, induces the production of interferon-gamma (IFN-gamma), favors the differentiation of T-helper 1 (Th1) cells and is an important link between innate resistance and adaptive immunity (PubMed : 8766560). Mechanistically, exerts its biological effects through a receptor composed of IL12R1 and IL12R2 subunits. Binding to the receptor results in the rapid tyrosine phosphorylation of a number of cellular substrates including the JAK family kinases TYK2 and JAK2. In turn, recruited STAT4 gets phosphorylated and translocates to the nucleus where it regulates cytokine/growth factor responsive genes (By similarity). As part of IL-35, plays essential roles in maintaining the immune homeostasis of the liver microenvironment and also functions as an immune-suppressive cytokine (PubMed : 18033300, PubMed : 30197594). Mediates biological events through unconventional receptors composed of IL12RB2 and gp130/IL6ST heterodimers or homodimers. Signaling requires the transcription factors STAT1 and STAT4, which form a unique heterodimer that binds to distinct DNA sites (By similarity).
See full target information Il12a

Additional targets

Il12b
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of immunology (Baltimore, Md. : 1950) 204:3400-3415 PubMed32358019

2020

Suppression of Mll1-Complex by Stat3/Cebpβ-Induced miR-21a/21b/181b Maintains the Accumulation, Homeostasis, and Immunosuppressive Function of Polymorphonuclear Myeloid-Derived Suppressor Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiqian Zhang,Xu Huang,Enlin Wang,Yugang Huang,Rongcun Yang

Microorganisms 7: PubMed31694208

2019

Isolation and Identification of HFY05 from Natural Fermented Yak Yogurt and Its Effect on Alcoholic Liver Injury in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ruokun Yi,Fang Tan,Wei Liao,Qiang Wang,Jianfei Mu,Xianrong Zhou,Zhennai Yang,Xin Zhao

American journal of translational research 11:280-289 PubMed30787986

2019

Efficacy of subcutaneous immunotherapy in a mouse model of Humulus pollen-induced allergic asthma.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Kong,Jia Yin

Journal of molecular microbiology and biotechnology 27:168-174 PubMed28772273

2017

Antitumor Effect of a DNA Vaccine Harboring Prostate Cancer-Specific Antigen with IL-12 as an Intramolecular Adjuvant.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Yang,Zhiqiang Shao,Jiangping Gao
View all publications
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