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AB119531

Mouse IL-12 p70 ELISA Kit

5

(1 Review)

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(8 Publications )

Mouse IL-12 p70 ELISA Kit is a sandwich ELISA designed to quantify Mouse IL-12 p70 with a sensitivity of 10 pg/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 15.6 - 1000 pg/mL

View Alternative Names

Interleukin-12 subunit alpha, IL-12A, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12 subunit p35, CLMF p35, Il12a

1 Images
Sandwich ELISA - Mouse IL-12 p70 ELISA Kit (AB119531)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-12 p70 ELISA Kit (AB119531)

Representative Standard Curve using ab119531.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 10 pg/mL

Range

15.6 - 1000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse IL-12 p70 ELISA Kit ab119531 is a sandwich ELISA to measure Mouse IL-12 p70 in serum, cell culture supernatant with a sensitivity of 10 pg/ml.

How the assay works

IL-12 p70 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with a Biotin-conjugated anti-mouse IL-12 p70 detection antibody and the microplate is then incubated at room temperature. Following washing with wash buffer a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and then unbound conjugates are washed away using wash buffer. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-12 p70 captured on the plate.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Mouse IL-12 p70 ELISA Kit ab119531 protocol summary

1. Add standard or sample to appropriate wells. Incubate the plate
2. Wash and add Biotinylated mouse anti-TGF beta 1 monoclonal antibody to appropriate wells. Incubate the plate.
3. Wash and add prepared Streptavidin-HRP Conjugate to appropriate wells. Incubate at room temperature
4. Wash and add TMB Substrate to each well
5. Add Stop Solution to each well. Read immediately

The standard is affinity purified.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 7, "mean": null, "standardDeviation": null, "coefficientOfVariability": "11" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 7, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.3" } ]
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Recovery

[ { "sample": "Serum", "range": "95 - 118 %", "average": "= 106" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab119531-1x96Tests", "assetComponentDetails": [ { "size":"1 x 50 mL", "name":"20X Wash Buffer Concentrate", "number":"AB119531-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Microplate coated with anti-mouse IL-12 p70 monoclonal antibody (12 x 8 wells)", "number":"AB119531-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent", "number":"AB119531-CMP06", "productcode":"" }, { "size":"4 x 1 Unit", "name":"Adhesive Films", "number":"AB119531-CMP01", "productcode":"" }, { "size":"1 x 5 mL", "name":"20X Assay Buffer Concentrate", "number":"AB119531-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"TMB Substrate Solution", "number":"AB119531-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"Stop Solution (1M Phosphoric acid)", "number":"AB119531-CMP07", "productcode":"" }, { "size":"1 x 70 µL", "name":"Biotin-Conjugate anti-mouse IL-12 p70 monoclonal antibody", "number":"AB119531-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse IL-12 p70 Standard, lyophilized (2 ng/mL on reconstitution).", "number":"AB119531-CMP05", "productcode":"" }, { "size":"1 x 150 µL", "name":"Streptavidin-HRP", "number":"AB119531-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Heterodimerizes with IL12B to form the IL-12 cytokine or with EBI3/IL27B to form the IL-35 cytokine. IL-12 is primarily produced by professional antigen-presenting cells (APCs) such as B-cells and dendritic cells (DCs) as well as macrophages and granulocytes and regulates T-cell and natural killer-cell responses, induces the production of interferon-gamma (IFN-gamma), favors the differentiation of T-helper 1 (Th1) cells and is an important link between innate resistance and adaptive immunity (PubMed : 8766560). Mechanistically, exerts its biological effects through a receptor composed of IL12R1 and IL12R2 subunits. Binding to the receptor results in the rapid tyrosine phosphorylation of a number of cellular substrates including the JAK family kinases TYK2 and JAK2. In turn, recruited STAT4 gets phosphorylated and translocates to the nucleus where it regulates cytokine/growth factor responsive genes (By similarity). As part of IL-35, plays essential roles in maintaining the immune homeostasis of the liver microenvironment and also functions as an immune-suppressive cytokine (PubMed : 18033300, PubMed : 30197594). Mediates biological events through unconventional receptors composed of IL12RB2 and gp130/IL6ST heterodimers or homodimers. Signaling requires the transcription factors STAT1 and STAT4, which form a unique heterodimer that binds to distinct DNA sites (By similarity).
See full target information Il12a
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

PloS one 20:e0322334 PubMed40323903

2025

Human cytomegalovirus-IE2 suppresses antigen presentation of macrophage through the IL10/STAT3 signalling pathway in transgenic mouse.

Applications

Unspecified application

Species

Unspecified reactive species

Xianjuan Zhang,Qing Wang,Shuo Han,Guanghui Song,Bin Wang,Yunyang Wang

Journal of orthopaedic surgery and research 19:517 PubMed39198853

2024

Novel insights into vancomycin-loaded calcium sulfate and negative pressure wound therapy in preventing infections in open fractures.

Applications

Unspecified application

Species

Unspecified reactive species

Bei Jia,Rui Xue,Jia Li,Jichao Guo,Jianning Liu

Scientific reports 13:296 PubMed36609424

2023

Immunogenicity of a secreted, C-terminally truncated, form of bovine viral diarrhea virus E2 glycoprotein as a potential candidate in subunit vaccine development.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Ting Lo,Martin D Ryan,Garry A Luke,Wan Chen Chang,Hsing Chieh Wu

Nature communications 13:3875 PubMed35790729

2022

Potentiating hypoxic microenvironment for antibiotic activation by photodynamic therapy to combat bacterial biofilm infections.

Applications

Unspecified application

Species

Unspecified reactive species

Weijun Xiu,Ling Wan,Kaili Yang,Xiao Li,Lihui Yuwen,Heng Dong,Yongbin Mou,Dongliang Yang,Lianhui Wang

Immunity, inflammation and disease 10:e624 PubMed35634952

2022

Effects of Astragalus membranaceus on systemic lupus erythematosus in a mouse model of pregnancy.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Qing Chen,Na Zhang,Cai-Xia Li,Hong-Xia Zhang

Pharmaceutical biology 60:326-333 PubMed35167426

2022

Tanshinone IIA alleviates ovalbumin-induced allergic rhinitis symptoms by inhibiting Th2 cytokine production and mast cell histamine release in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Chen,Liping Shao,Yong Li,Mian Dai,He Liu,Nan Xiang,Hui Chen

Nutrients 10: PubMed30201943

2018

Polyphenols in Liubao Tea Can Prevent CCl₄-Induced Hepatic Damage in Mice through Its Antioxidant Capacities.

Applications

Unspecified application

Species

Unspecified reactive species

Yanni Pan,Xingyao Long,Ruokun Yi,Xin Zhao

International immunopharmacology 57:157-164 PubMed29499453

2018

Gold‑manganese oxide nanocomposite suppresses hypoxia and augments pro-inflammatory cytokines in tumor associated macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Anupam Nath,Ramkrishna Pal,Leichombam Mohindro Singh,Himadri Saikia,Hasimur Rahaman,Sujit Kumar Ghosh,Ritwik Mazumder,Mahuya Sengupta
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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