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AB219634

Mouse IL-13 ELISA Kit

4

(1 Review)

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(20 Publications)

Mouse IL-13 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse IL-13 with a sensitivity of 1.2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 20 citations

View Alternative Names

Il-13, Il13, Interleukin-13, IL-13, T-cell activation protein P600

5 Images
ELISA - Mouse IL-13 ELISA Kit (AB219634)
  • ELISA

Supplier Data

ELISA - Mouse IL-13 ELISA Kit (AB219634)
Sandwich ELISA - Mouse IL-13 ELISA Kit (AB219634)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-13 ELISA Kit (AB219634)

Interpolated concentrations of native IL-13 in mouse EL4/IL-2 cell culture supernatant unstimulated and PHA/PMA stimulated samples.

The concentrations of IL-13 were measured in duplicates, interpolated from the IL-13 standard curves and corrected for sample dilution. Undiluted samples are 2%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). T The mean IL-13 concentration was determined to be 3144 pg/mL in PHA/PMA stimulated EL4/IL-2 cell culture supernatant and undetermined in media and unstimulated cell culture supernatant.

Sandwich ELISA - Mouse IL-13 ELISA Kit (AB219634)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-13 ELISA Kit (AB219634)

Interpolated concentrations of native IL-13 in mouse serum and plasma samples.

The concentrations of IL-13 were measured in duplicates, interpolated from the IL-13 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 10%, plasma (citrate) 10%, plasma (EDTA) 10% and plasma (heparin) 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-13 concentration was determined to be 31.5 pg/mL in serum, 21.1 pg/mL in plasma (citrate) and 20.4 pg/mL in plasma (EDTA) and 29.1 pg/mL in plasma (heparin).

Sandwich ELISA - Mouse IL-13 ELISA Kit (AB219634)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-13 ELISA Kit (AB219634)

Example of mouse IL-13 standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse IL-13 ELISA Kit (AB219634)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-13 ELISA Kit (AB219634)

Interpolated concentrations of spike IL-13 in mouse serum and plasma samples.

The concentrations of IL-13 were measured in duplicates, interpolated from the IL-13 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 10%, plasma (citrate) 10%, plasma (EDTA) 10% and plasma (heparin) 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 1.2 pg/mL

Range

1.95 - 125 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

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Product details

Mouse IL-13 ELISA Kit ab219634 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse IL-13 in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 1.2 pg/mL.

How the assay works

Mouse IL-13 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse IL-13 ELISA Kit ab219634 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-IL-13 antibody [EPR20435-21] - BSA and Azide free (Capture) ab242908
- Anti-IL-13 antibody [EPR20435-4] - BSA and Azide free (Detector) ab242908

Interleukin-13 (IL-13) is a secreted immunoregulatory cytokine in the IL-4/IL-13 family that is linked to allergic reactions and cancer pathogenesis. IL-13 inhibits inflammatory cytokine production and promotes immunoglobulin class switching on B-cells. IL-13 synergizes with IL2 in regulating interferon-gamma synthesis. IL-13 is linked to allergic rhinitis, a common disease of complex inheritance characterized by mucosal inflammation caused by allergen exposure.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.4" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.2" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "106 - 111 %", "average": "= 108" }, { "sample": "Serum", "range": "95 - 98 %", "average": "= 97" }, { "sample": "EDTA Plasma", "range": "97 - 99 %", "average": "= 98" }, { "sample": "Heparin Plasma", "range": "101 - 108 %", "average": "= 104" }, { "sample": "Citrate plasma", "range": "95 - 100 %", "average": "= 97" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Cytokine that plays important roles in allergic inflammation and immune response to parasite infection (PubMed : 15361238). Synergizes with IL2 in regulating interferon-gamma synthesis. Stimulates B-cell proliferation, and activation of eosinophils, basophils, and mast cells (By similarity). Plays an important role in controlling IL33 activity by modulating the production of transmembrane and soluble forms of interleukin-1 receptor-like 1/IL1RL1 (PubMed : 34789557). Displays the capacity to antagonize Th1-driven proinflammatory immune response and downregulates synthesis of many proinflammatory cytokines including IL1, IL6, IL10, IL12 and TNF-alpha through a mechanism that partially involves suppression of NF-kappa-B (By similarity). Functions also on nonhematopoietic cells, including endothelial cells where it induces vascular cell adhesion protein 1/VCAM1, which is important in the recruitment of eosinophils. Exerts its biological effects through its receptors which comprises the IL4R chain and the IL13RA1 chain, to activate JAK1 and TYK2, leading to the activation of STAT6 (PubMed : 34795444, PubMed : 8871614). Aside from IL13RA1, another receptor IL13RA2 acts as a high affinity decoy for IL13 and mediates internalization and depletion of extracellular IL13 (PubMed : 29305434).
See full target information Il13
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Publications (20)

Recent publications for all applications. Explore the full list and refine your search

iScience 26:106953 PubMed37332598

2023

SENP3-mediated deSUMOylation of c-Jun facilitates microglia-induced neuroinflammation after cerebral ischemia and reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Xia,Meng Mao,Gaofeng Zhan,Zhenzhao Luo,Yin Zhao,Xing Li

Allergologia et immunopathologia 51:146-153 PubMed36617834

2023

Chryseriol attenuates the progression of OVA-induced asthma in mice through NF-κB/HIF-1α and MAPK/STAT1 pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Shangyao Mo,Hao Deng,Yong Xie,Lixia Yang,Lili Wen

Allergy, asthma, and clinical immunology : official journal of the Canadian Society of Allergy and Clinical Immunology 18:88 PubMed36184652

2022

Anti-asthmatic miR-224-5p inhibits the FHL1/MAPK pathway to repress airway smooth muscle cell proliferation in a murine model of asthma-like airway inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Zhifang Zhuang,Yanjuan Zhou,Jiao Xu,Leying Pan

Allergologia et immunopathologia 50:75-83 PubMed36086967

2022

Zeaxanthin attenuates OVA-induced allergic asthma in mice by regulating the p38 MAPK/β-catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaosheng Jin,Weijing Jin,Guoping Li,Jisheng Zheng

Analytical cellular pathology (Amsterdam) 2022:3066456 PubMed35915851

2022

XBP1 Regulates the Transcription of HIF-1a in BALB/c Mice with Chronic Rhinosinusitis without Polyps.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaopeng Qu,Hongyan Li,Lingzhao Meng

Annals of translational medicine 10:209 PubMed35280398

2022

Polyinosinic:polycytidylic acid aggravates calcipotriol-induced atopic dermatitis-like skin lesions in mice by increasing the expression of thymic stromal lymphopoietin.

Applications

Unspecified application

Species

Unspecified reactive species

Haoyue Wan,Huixue Yang,Mingjing Wei,Wenqi Chen

Molecular medicine reports 25: PubMed35103289

2022

Pulsatilla decoction alleviates colitis by enhancing autophagy and regulating PI3K‑Akt‑mTORC1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xuewei Wang,Lijun Xu,Tao Wang,Jian Xu,Fugang Fan,Yu Zhang,Jinpin Wang,Qin Cao

Experimental and therapeutic medicine 23:193 PubMed35126696

2022

Lidocaine relieves murine allergic rhinitis by regulating the NF-κB and p38 MAPK pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Xiang,Zhen Yang,Qiang Zhou

International archives of allergy and immunology 183:375-388 PubMed34942624

2021

Mechanism of miR-181a-5p in Regulatory T/T-Helper 17 Immune Imbalance and Asthma Development in Mice with Allergic Rhinitis.

Applications

Unspecified application

Species

Unspecified reactive species

Ruoyun He,Yujuan Chen,Xiaoer Chen,Binfan Yuan

Asian Pacific journal of cancer prevention : APJCP 22:3647-3661 PubMed34837924

2021

Chemopreventive Role of Black Tea Extract in Swiss Albino Mice Exposed to Inorganic Arsenic.

Applications

Unspecified application

Species

Unspecified reactive species

Archismaan Ghosh,Sutapa Mukherjee,Madhumita Roy
View all publications
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