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AB275894

Mouse IL-13R alpha 2 ELISA Kit

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Mouse IL-13R alpha 2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse IL-13R alpha 2 with a sensitivity of 26.31 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD213a2, Interleukin-13 receptor subunit alpha-2, IL-13 receptor subunit alpha-2, IL-13R subunit alpha-2, IL-13R-alpha-2, IL-13RA2, Il13ra2

4 Images
Sandwich ELISA - Mouse IL-13R alpha 2 ELISA Kit (AB275894)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-13R alpha 2 ELISA Kit (AB275894)

Interpolated concentrations of native L-13R alpha 2 in mouse serum and plasma samples.

The concentrations of IL-13R alpha 2 were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 10%, plasma (citrate) 20%, plasma (heparin) 10%, and plasma (EDTA) 20%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 29.01 ng/ml in serum, 12.39 ng/ml in plasma (citrate), 23.70 ng/ml in plasma (heparin), and 18.01 ng/ml plasma (EDTA).

Sandwich ELISA - Mouse IL-13R alpha 2 ELISA Kit (AB275894)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-13R alpha 2 ELISA Kit (AB275894)

Interpolated concentrations of native IL-13R alpha 2 in mouse spleen tissue homogenate extract samples based on a 500 μg/mL extract load.

The concentrations of IL-13R alpha 2 were measured in duplicate and interpolated from the IL-13R alpha 2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-13R alpha 2 concentration was determined to be 241.29 pg/mL in mouse spleen tissue homogenate extract.

Sandwich ELISA - Mouse IL-13R alpha 2 ELISA Kit (AB275894)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-13R alpha 2 ELISA Kit (AB275894)

Human IL-13R alpha 2 competitor curve comparison.

Standard Curve comparison between Mouse IL-13R alpha 2 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

Sandwich ELISA - Mouse IL-13R alpha 2 ELISA Kit (AB275894)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-13R alpha 2 ELISA Kit (AB275894)

Example of mouse IL-13R alpha 2 standard curve in Sample Diluent NS.

The IL-13R alpha 2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 26.31 pg/mL

Range

101.56 - 6500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse IL-13R alpha 2 ELISA kit (ab275894) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse IL-13R alpha 2 protein in mouse serum, plasma - citrate, plasma - heparin, plasma - edta, cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IL-13R alpha 2 with 26.31 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less

-High sensitivity, specificity and reproducibility from superior antibodies

-Fully validated in biological samples

-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

IL-13R alpha 2 is a cytokine receptor involved in Th2 responses during parasitic infections and allergic asthma. IL-13R alpha 2 is released into bronchoalveolar lavage fluid from house dust mite allergen. It is also upregulated during heart failure, pulmonary hypertension, and in some forms of cancer.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.1" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.4" } ]
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Recovery

[ { "sample": "Serum", "range": "83 - 103 %", "average": "= 94" }, { "sample": "EDTA Plasma", "range": "102 - 111 %", "average": "= 106" }, { "sample": "Cell culture media", "range": "98 - 109 %", "average": "= 103" }, { "sample": "Heparin Plasma", "range": "93 - 108 %", "average": "= 99" }, { "sample": "Citrate plasma", "range": "97 - 103 %", "average": "= 100" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab275894-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB275894-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB275894-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB275894-CMP11", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB275894-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB275894-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB275894-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse IL-13R alpha 2 Lyophilized Recombinant Protein", "number":"AB275894-CMP02", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB275894-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse IL-13R alpha 2 Capture Antibody", "number":"AB275894-CMP08", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB275894-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse IL-13R alpha 2 Detector Antibody", "number":"AB275894-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Cell surface receptor that plays a role in the regulation of IL-13-mediated responses (PubMed : 12642602). Functions as a decoy receptor that inhibits IL-13- and IL-4-mediated signal transduction via the JAK-STAT pathway and thereby modulates immune responses and inflammation. Serves as a functional signaling receptor for IL-13 in an alternative pathway involving AP-1 ultimately leading to the production of TGFB1.
See full target information Il13ra2
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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