AB275898

Mouse IL-15 ELISA Kit

Recombinant
SimpleStep
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(1 Publication)

Mouse IL-15 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse IL-15 with a sensitivity of 45.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Interleukin-15, IL-15, Il15

4 Images

ELISA - Mouse IL-15 ELISA Kit (AB275898)

ELISA

Supplier Data

ELISA - Mouse IL-15 ELISA Kit (AB275898)

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-15 ELISA Kit (AB275898)

Example of mouse IL-15 standard curve in Sample Diluent NS + 1X Enhancer

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-15 ELISA Kit (AB275898)

Interpolated concentrations of spike IL-15 in mouse serum and plasma samples

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-15 ELISA Kit (AB275898)

Representative image of Mouse IL-15 ELISA Kit (ab275898)

Components shown from left to right :

- Sample diluent NS

- 10X Mouse IL-15 Capture Antibody

- 10X Mouse IL-15 Detector Antibody

- Mouse IL-15 Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Stop Solution

- TMB solution

- 10X Wash Buffer PT (ab206977)

- Antibody Diluent 4BR

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 45.4 pg/mL

Range

131.3 - 8400 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse IL-15 ELISA Kit ab275898 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse IL-15 in cell culture media, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 45.4 pg/mL.

How the assay works

Mouse IL-15 SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse IL-15 ELISA Kit ab275898 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-IL-15 antibody [EPR22635-211] - BSA and Azide free (Capture) ab276164
- Anti-IL-15 antibody [EPR22635-101] - BSA and Azide free (Detector) ab276164

Interleukin (IL)-15 is a proinflammatory cytokine found expressed in a large number of cell types and tissues, and mainly secreted by mononuclear phagocytes. IL-15 acts as a pleiotropic cytokine that plays a vital role in the innate and adaptive immunity. Main function of IL-15 is to stimulate cell activation and proliferation of natural killer cells by signaling through IL-15 receptor and T-lymphocytes by interacting with IL-2 beta and gamma components.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.53" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.1" } ]

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Recovery

[ { "sample": "Serum", "range": "80 - 84 %", "average": "= 82" }, { "sample": "EDTA Plasma", "range": "79 - 86 %", "average": "= 83" }, { "sample": "Cell culture media", "range": "107 - 108 %", "average": "= 108" }, { "sample": "Heparin Plasma", "range": "100 - 106 %", "average": "= 103" }, { "sample": "Citrate plasma", "range": "79 - 81 %", "average": "= 80" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab275898-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB275898-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB275898-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB275898-CMP11", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB275898-CMP07", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB275898-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB275898-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB275898-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse IL-15 Capture Antibody", "number":"AB275898-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse IL-15 Lyophilized Recombinant Protein", "number":"AB275898-CMP02", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB275898-CMP01", "productcode":"" }, { "size":"1 x 600 mL", "name":"10X Mouse IL-15 Detector Antibody", "number":"AB275898-CMP09", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Cytokine that plays a major role in the development of inflammatory and protective immune responses to microbial invaders and parasites by modulating immune cells of both the innate and adaptive immune systems (PubMed : 11466332, PubMed : 8757333). Stimulates the proliferation and activation of natural killer cells, T-cells and B-cells and promotes the secretion of several cytokines (PubMed : 8757333). In monocytes, induces the production of IL8 and monocyte chemotactic protein 1/CCL2, two chemokines that attract neutrophils and monocytes respectively to sites of infection (By similarity). Unlike most cytokines, which are secreted in soluble form, IL15 is expressed in association with its high affinity IL15RA on the surface of IL15-producing cells and delivers signals to target cells that express IL2RB and IL2RG receptor subunits. Binding to its receptor triggers the phosphorylation of JAK1 and JAK3 and the recruitment and subsequent phosphorylation of signal transducer and activator of transcription-3/STAT3 and STAT5. In mast cells, induces the rapid tyrosine phosphorylation of STAT6 and thereby controls mast cell survival and release of cytokines such as IL4 (PubMed : 10882748, PubMed : 19632221).

See full target information Il15

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biology direct 19:48 PubMed38902802

2024

PNSC928, a plant-derived compound, specifically disrupts CtBP2-p300 interaction and reduces inflammation in mice with acute respiratory distress syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Li,Wenqing Yan,Weihua Dong,Zhiping Chen,Zhi Chen

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse IL-15 ELISA Kit

ELISA - Mouse IL-15 ELISA Kit (AB275898)

Sandwich ELISA - Mouse IL-15 ELISA Kit (AB275898)

Sandwich ELISA - Mouse IL-15 ELISA Kit (AB275898)

Sandwich ELISA - Mouse IL-15 ELISA Kit (AB275898)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Assay Kits

Assay Kits

Secondary Antibodies

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (1)

Product promise