AB326908
Mouse IL-15 ELISA Kit, Chemiluminescent
- SimpleStep Ignite™ ELISA
- Recombinant
- What is this?
Be the first to review this product! Submit a review
|
(0 Publication)
Mouse IL-15 ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Mouse IL-15 with higher sensitivity and broader measuring range.
- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 16 - 35000 pg/ml with a sensitivity of 7.1 pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending
View Alternative Names
Interleukin-15, IL-15, Il15
3 Images
- sELISA
Lab
Sandwich ELISA - Mouse IL-15 ELISA Kit, Chemiluminescent (AB326908)
Dynamic range comparison between the Chemiluminescent Mouse IL-15 SimpleStep ELISA, colorimetric Mouse IL-15 SimpleStep ELISA (ab275898), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows comparable sensitivity and increased dynamic range.
- sELISA
Lab
Sandwich ELISA - Mouse IL-15 ELISA Kit, Chemiluminescent (AB326908)
Standard curve comparison between the Chemiluminescent Mouse IL-15 SimpleStep ELISA and colorimetric Mouse IL-15 SimpleStep ELISA (ab275898) in Sample Diluent NS + 1X Enhancer. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Mouse IL-15 ELISA Kit, Chemiluminescent (AB326908)
Interpolated concentrations of spike IL-15 in mouse serum and plasma samples
Reactivity data
{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
Product details
Mouse IL-15 ELISA Kit, Chemiluminescent (ab326908) is a rapid single-wash sandwich 55-min ELISA to measure Mouse IL-15 in Serum, EDTA Plasma, Cell culture media, Heparin Plasma, Citrate plasma with a sensitivity of 7.1 pg/mL.
How the assay works
Mouse IL-15 ELISA Kit, Chemiluminescent (ab326908) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.
Assay Specificity
Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Mouse IL-15 (ab326908) protocol summary
1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-IL-15 antibody [EPR22635-211] - BSA and Aside free (Capture) ab276163
- Anti-IL-15 antibody [EPR22635-101] - BSA and Aside free (Detector) ab276164
How the assay works
Mouse IL-15 ELISA Kit, Chemiluminescent (ab326908) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.
Assay Specificity
Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Mouse IL-15 (ab326908) protocol summary
1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-IL-15 antibody [EPR22635-211] - BSA and Aside free (Capture) ab276163
- Anti-IL-15 antibody [EPR22635-101] - BSA and Aside free (Detector) ab276164
Precision
[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 5.1" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 5.53" } ]
Recovery
[ { "sample": "Serum", "range": "80 - 84 %", "average": "= 84" }, { "sample": "EDTA Plasma", "range": "79 - 86 %", "average": "= 86" }, { "sample": "Cell culture media", "range": "107 - 108 %", "average": "= 108" }, { "sample": "Heparin Plasma", "range": "100 - 106 %", "average": "= 106" }, { "sample": "Citrate plasma", "range": "79 - 81 %", "average": "= 81" } ]
What's included?
{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab326908-1x96Tests", "assetComponentDetails": [ { "size":"1 x 600 µL", "name":"10X Mouse IL-15 Detector Antibody", "number":"AB326908-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB326908-CMP11", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent A", "number":"AB326908-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB326908-CMP10", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB326908-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB326908-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB326908-CMP04", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent B", "number":"AB326908-CMP09", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB326908-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse IL-15 Capture Antibody", "number":"AB326908-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse IL-15 Lyophilized Recombinant Protein", "number":"AB326908-CMP03", "productcode":"" } ] } } }
Properties and storage information
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Cytokine that plays a major role in the development of inflammatory and protective immune responses to microbial invaders and parasites by modulating immune cells of both the innate and adaptive immune systems (PubMed : 11466332, PubMed : 8757333). Stimulates the proliferation and activation of natural killer cells, T-cells and B-cells and promotes the secretion of several cytokines (PubMed : 8757333). In monocytes, induces the production of IL8 and monocyte chemotactic protein 1/CCL2, two chemokines that attract neutrophils and monocytes respectively to sites of infection (By similarity). Unlike most cytokines, which are secreted in soluble form, IL15 is expressed in association with its high affinity IL15RA on the surface of IL15-producing cells and delivers signals to target cells that express IL2RB and IL2RG receptor subunits. Binding to its receptor triggers the phosphorylation of JAK1 and JAK3 and the recruitment and subsequent phosphorylation of signal transducer and activator of transcription-3/STAT3 and STAT5. In mast cells, induces the rapid tyrosine phosphorylation of STAT6 and thereby controls mast cell survival and release of cytokines such as IL4 (PubMed : 10882748, PubMed : 19632221).
Product promise
We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com