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AB100703

Mouse IL-17B ELISA Kit

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Mouse IL-17B ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse IL-17B in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Nirf, Zcyto7, Il17b, Interleukin-17B, IL-17B, Cytokine CX1, Cytokine-like protein ZCYTO7, Neuronal interleukin-17-related factor

1 Images
Sandwich ELISA - Mouse IL-17B ELISA Kit (AB100703)
  • sELISA

Unknown

Sandwich ELISA - Mouse IL-17B ELISA Kit (AB100703)

Representative standard curve using ab100703

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

< 12 pg/mL

Range

16.46 - 12000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's IL-17B Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-17B in serum (mouse IL-17B concentration is pretty low in normal serum/plasma, it may not be detected in this assay), plasma and cell culture supernatants.

This assay employs an antibody specific for mouse IL-17B coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-17B present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-17B antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-17B bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "139 - 161 %", "average": "= 149.4" }, { "sample": "Serum", "range": "104 - 128 %", "average": "= 118.9" }, { "sample": "Plasma", "range": "92 - 155 %", "average": "= 129.3" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100703-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100703-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100703-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IL-17B Microplate (12 x 8 wells)", "number":"AB100703-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse IL-17B Standard (lyophilized)", "number":"AB100703-CMP02", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB100703-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100703-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-mouse IL-17B", "number":"AB100703-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D", "number":"AB100703-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100703-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Stimulates the release of tumor necrosis factor alpha and IL-1-beta from the monocytic cell line THP-1.
See full target information Il17b
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websiteProtocolBooklet
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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