Mouse IL-17C ELISA Kit

Product details

The Mouse IL-17C Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213868) is designed for the quantitative measurement of Mouse IL-17C in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-17C has been pre-coated onto 96-well plates. Standards (Expression system for standard: E.coli; Immunogen sequence: D17-Q194) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-17C is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse IL-17C amount of sample captured in plate.

IL-17C, also known as CX2, is a protein that in humans is encoded by the IL-17C gene. IL-17C is mapped to 16q24.3. The protein encoded by this gene is a T cell-derived cytokine that shares the sequence similarity with IL-17. This cytokine was reported to stimulate the release of tumor necrosis factor alpha and interleukin 1 beta from a monocytic cell line. The expression of this cytokine was found to be restricted to activated T cells. IL-17C is an essential autocrine cytokine regulating innate epithelial immune responses. It also plays an important role in the pathogenesis of inflammatory arthritis.