Mouse IL-17RB ELISA Kit

Product details

Mouse IL-17RB ELISA Kit is designed for the quantitative determination of IL-17RB in cell culture supernatants, plasma and serum samples.

Δ Note: Mouse IL-17RB concentration is low in normal serum/plasma and may not be detectable in this assay.

This assay employs an antibody specific for mouse IL-17RB coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-17RB present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-17RB antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-17RB bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse Adiponectin (ACRP30), ANGPTL1, ANGPTL3, Artemin, CCL28 (MEC), CD36 (SRB3), Chordin, CRP, E-Cadherin, Epigen, Epiregulin, Fas, Galectin-7, gp130, Granzyme B, Gremlin-1, IFN-gamma R1, IL-17B, IL-22, MIP-1beta, MMP-10, MMP-2, MMP-3, PDGF-AA, Persephin, sFRP-3, SLAM, Shh-N, TCK-1, TECK, TGF beta 1, Trance, TREML1, TWEAK, VEGF-B, VEGFR2.