AB216165

Mouse IL-18 ELISA Kit

Recombinant
SimpleStep
What is this?

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(73 Publications)

Mouse IL-18 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse IL-18 with a sensitivity of 10.5 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 70 citations

View Alternative Names

Igif, Il18, Interleukin-18, IL-18, Interferon gamma-inducing factor, Interleukin-1 gamma, IFN-gamma-inducing factor, IL-1 gamma

15 Images

ELISA - Mouse IL-18 ELISA Kit (AB216165)

ELISA

Supplier Data

ELISA - Mouse IL-18 ELISA Kit (AB216165)

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-18 ELISA Kit (AB216165)

Example of mouse IL-18 standard curve in Sample Diluent NS.

The IL-18 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-18 ELISA Kit (AB216165)

Example of mouse IL-18 standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-18 ELISA Kit (AB216165)

Assay sensitivity.

The MDD was determined by calculating the mean of zero standard replicates and adding 2 standard deviations then extrapolating the corresponding concentration.

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-18 ELISA Kit (AB216165)

Interpolated concentrations of native IL-18 in mouse spleen extract sample based on a 100 μg/mL extract load.

The concentrations of IL-18 were measured in duplicate and interpolated from the IL-18 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-18 concentration was determined to be 1882 pg/mL in spleen extract.

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-18 ELISA Kit (AB216165)

Interpolated concentrations of spike IL-18 in mouse serum and plasma samples.

The concentrations of IL-18 were measured in duplicates, interpolated from the IL-18 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-18 ELISA Kit (AB216165)

Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

Recombinant IL-18 was spiked into the following biological samples and diluted in a 2-fold dilution series in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-18 ELISA Kit (AB216165)

Example of mouse IL-18 standard curve in 1X Cell Extraction Buffer PTR.

The IL-18 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-18 ELISA Kit (AB216165)

Linearity of dilution.

Native IL-18 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-18 ELISA Kit (AB216165)

Interpolated concentrations of native IL-18 in mouse serum and plasma samples.

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-18 ELISA Kit (AB216165)

Example of mouse IL-18 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-18 ELISA Kit (AB216165)

Interpolated concentrations of spike IL-18 in mouse cell culture media sample.

The concentrations of IL-18 were measured in duplicates, interpolated from the IL-18 standard curves and corrected for sample dilution. Undiluted samples are as follows : Cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Lab

Sandwich ELISA - Mouse IL-18 ELISA Kit (AB216165)

Example of mouse IL-18 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Mouse IL-18 ELISA Kit (AB216165)

Example of mouse IL-18 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Mouse IL-18 ELISA Kit (AB216165)

Interpolated concentration of native IL-18 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 75 µg/mL mouse spleen extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 10.5 pg/mL

Range

31.3 - 2000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse IL-18 ELISA Kit ab216165 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse IL-18 in cell culture extracts, cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum, tissue extracts. This SimpleStep sensitivity is 10.5 pg/mL.

How the assay works

Mouse IL-18 SimpleStep ELISA^®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse IL-18 ELISA Kit ab216165 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-IL-18 antibody [EPR19956-92] - BSA and Azide free (Capture) ab242856
- Anti-IL-18 antibody [EPR19956-19] - BSA and Azide free (Detector) ab242856

Interleukin-18 (IL-18 or Interferon gamma-inducing factor) is a proinflammatory cytokine in the IL-1 superfamily. IL-18 has overlapping and synergistic biological function with IL-12. IL-18 is found in both intracellular and extracellular compartments. The antibodies in this kit were raised to the mature full-length sequence of mouse IL-18.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.8" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.4" } ]

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Recovery

[ { "sample": "Serum", "range": "88 - 115 %", "average": "= 100" }, { "sample": "EDTA Plasma", "range": "84 - 91 %", "average": "= 87" }, { "sample": "Cell culture extracts", "range": "91 - 108 %", "average": "= 100" }, { "sample": "Tissue Extracts", "range": "102 - 117 %", "average": "= 110" }, { "sample": "Heparin Plasma", "range": "88 - 95 %", "average": "= 90" }, { "sample": "Citrate plasma", "range": "86 - 97 %", "average": "= 88" }, { "sample": "Cell culture media", "range": "103 - 113 %", "average": "= 107" } ]

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What's included?

{ "values": { "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab216165-1x384Tests", "assetComponentDetails": [ { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB216165-CMP04" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB216165-CMP06" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB216165-CMP13" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB216165-CMP02" }, { "size":"2 x 1 Vial", "name":"Mouse IL-18 Lyophilized Recombinant Protein", "number":"AB216165-CMP01" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB216165-CMP12" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB216165-CMP11" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB216165-CMP05" }, { "size":"1 x 50 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB216165-CMP07" }, { "size":"1 x 600 µL", "name":"10X Mouse IL-18 Capture Antibody", "number":"AB216165-CMP09" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB216165-CMP08" }, { "size":"1 x 600 µL", "name":"10X Mouse IL-18 Detector Antibody", "number":"AB216165-CMP10" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab216165-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB216165-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB216165-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB216165-CMP06", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB216165-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB216165-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB216165-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse IL-18 Lyophilized Recombinant Protein", "number":"AB216165-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse IL-18 Capture Antibody", "number":"AB216165-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB216165-CMP08", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB216165-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB216165-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse IL-18 Detector Antibody", "number":"AB216165-CMP10", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Pro-inflammatory cytokine primarily involved in epithelial barrier repair, polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses (PubMed : 26638072, PubMed : 26638073). Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators (By similarity). Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells (By similarity). Involved in transduction of inflammation downstream of pyroptosis : its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore (PubMed : 30392956).

See full target information Il18

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Publications (73)

Recent publications for all applications. Explore the full list and refine your search

BMC cardiovascular disorders 25:176 PubMed40082765

2025

Impdh1 was identified as a key protein promotes diabetic vasculopathy by intervention of vascular endothelial cell pyroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Ruiqiang Xie,Hong Gao,Hongyan Xie,Chunguang Xie,Tianhao Li

Journal of orthopaedic surgery and research 20:198 PubMed40001262

2025

Mechanism of lncRNA ZFAS1 mediating nucleus pulposus cell pyroptosis in intervertebral disc degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Yuchun Fu,Leilei Zhu,Bingxu Ma

BMC gastroenterology 25:54 PubMed39910460

2025

Helicobacter pylori CagA+ strains modulate colorectal pathology by regulating intestinal flora.

Applications

Unspecified application

Species

Unspecified reactive species

Shasha Cui,Xinqiang Liu,Fengxia Han,Lu Zhang,Jingjing Bu,Sainan Wu,Jiafen Wang

Redox biology 80:103517 PubMed39879739

2025

Methamphetamine-mediated astrocytic pyroptosis and neuroinflammation involves miR-152-NLRP6 inflammasome signaling axis.

Applications

Unspecified application

Species

Unspecified reactive species

Abiola Oladapo,Muthukumar Kannan,Uma Maheswari Deshetty,Seema Singh,Shilpa Buch,Palsamy Periyasamy

Redox biology 78:103418 PubMed39531828

2024

Deficiency of muscle-generated brain-derived neurotrophic factor causes inflammatory myopathy through reactive oxygen species-mediated necroptosis and pyroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Brian Pak Shing Pang,Elsie Chit Yu Iu,Miaojia Hang,Wing Suen Chan,Margaret Chui Ling Tse,Connie Tsz Ying Yeung,Mingfu Wang,Parco Ming Fai Siu,Chi Wai Lee,Keqiang Ye,Ho So,Chi Bun Chan

Molecular medicine (Cambridge, Mass.) 30:155 PubMed39300342

2024

CircMAPK1 induces cell pyroptosis in sepsis-induced lung injury by mediating KDM2B mRNA decay to epigenetically regulate WNK1.

Applications

Unspecified application

Species

Unspecified reactive species

Min Li,Hanjing Lu,Chujun Ruan,Qiao Ke,Longhui Hu,Zhao Li,Xiaoran Liu

Allergologia et immunopathologia 52:21-28 PubMed39278847

2024

extract relieved sepsis-induced myocardial ferroptosis and inflammation by inhibiting lncRNA MIAT/TRAF6/NF-κB axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yao Shi,Xiaobo Yang,Hong Jiang,Shanxia Wu,Yan Hong,Wei Su,Xuan Wang

Journal of neuroinflammation 21:223 PubMed39277762

2024

Targeting the NLRP3 inflammasome in cochlear macrophages protects against hearing loss in chronic suppurative otitis media.

Applications

Unspecified application

Species

Unspecified reactive species

Viktoria Schiel,Ritwija Bhattacharya,Ankur Gupta,Kourosh Eftekharian,Anping Xia,Peter L Santa Maria

Cell death & disease 15:400 PubMed38849380

2024

Gasdermin D silencing alleviates airway inflammation and remodeling in an ovalbumin-induced asthmatic mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Jinxiang Wu,Pin Wang,Xinyu Xie,Xiaoqi Yang,Shuangmei Tang,Jiping Zhao,Tian Liu,Junfei Wang,Jintao Zhang,Tongliang Xia,Xin Feng

Cell 187:2785-2800.e16 PubMed38657604

2024

Synthetic protein circuits for programmable control of mammalian cell death.

Applications

Unspecified application

Species

Unspecified reactive species

Shiyu Xia,Andrew C Lu,Victoria Tobin,Kaiwen Luo,Lukas Moeller,D Judy Shon,Rongrong Du,James M Linton,Margaret Sui,Felix Horns,Michael B Elowitz

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse IL-18 ELISA Kit

ELISA - Mouse IL-18 ELISA Kit (AB216165)

Sandwich ELISA - Mouse IL-18 ELISA Kit (AB216165)

Sandwich ELISA - Mouse IL-18 ELISA Kit (AB216165)

Sandwich ELISA - Mouse IL-18 ELISA Kit (AB216165)

Sandwich ELISA - Mouse IL-18 ELISA Kit (AB216165)

Sandwich ELISA - Mouse IL-18 ELISA Kit (AB216165)

Sandwich ELISA - Mouse IL-18 ELISA Kit (AB216165)

Sandwich ELISA - Mouse IL-18 ELISA Kit (AB216165)

Sandwich ELISA - Mouse IL-18 ELISA Kit (AB216165)

Sandwich ELISA - Mouse IL-18 ELISA Kit (AB216165)

Sandwich ELISA - Mouse IL-18 ELISA Kit (AB216165)

Sandwich ELISA - Mouse IL-18 ELISA Kit (AB216165)

Sandwich ELISA - Mouse IL-18 ELISA Kit (AB216165)

Sandwich ELISA - Mouse IL-18 ELISA Kit (AB216165)

Sandwich ELISA - Mouse IL-18 ELISA Kit (AB216165)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (73)

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