Mouse IL-18BP ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse IL-18BP with a sensitivity of 151.82 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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Key facts
- Detection method
- Colorimetric
- Sample types
- Citrate plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 468.75 - 30000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 151.82 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Binds to IL-18 and inhibits its activity. Functions as an inhibitor of the early TH1 cytokine response (By similarity).
Alternative names
Igifbp, Il18bp, Interleukin-18-binding protein, IL-18BP, Interferon gamma-inducing factor-binding protein
What's included?
Recommended products
Mouse IL-18BP ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse IL-18BP with a sensitivity of 151.82 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Citrate plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 468.75 - 30000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 151.82 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 8 | C.V. 3.7 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 97 | Range |
Sample type Citrate plasma | Average % = 98 | Range |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse IL-18BP ELISA Kit (ab254509) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-18BP protein in cit plasma and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IL-18BP with 151.82 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
IL-18BP is a protein that binds to IL-18, preventing it from interacting with its receptor. IL-18BP inhibits the early TH1 cytokine response. IL-18 is produced constitutively by mononuclear cells.
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2 product images
Sandwich ELISA - Mouse IL-18BP ELISA Kit (ab254509)
Example of mouse IL-18BP standard curve in Sample Diluent NS.
The IL-18BP standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse IL-18BP ELISA Kit (ab254509)
Interpolated concentrations of native IL-18BP in mouse serum and plasma (citrate) samples.
The concentrations of IL-18BP were measured in duplicates, interpolated from the IL-18BP standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 2.5% and plasma (citrate) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-18BP concentration was determined to 175.87 ng/mL in serum and 201.01 ng/mL in plasma (citrate).
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Product protocols
- Visit the general protocols
- Visit troubleshooting
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