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Mouse IL-18BP ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse IL-18BP in Citrate plasma, Serum samples.
Alternative names=Interleukin-18-binding protein, IL-18BP, Interferon gamma-inducing factor-binding protein, Igifbp, Il18bp
- Detection method
Colorimetric
- Sample types
Citrate plasma, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Mouse
- Range
468.75 - 30000 pg/mL
- Assay time
1h 30m
- Sensitivity
= 151.82 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Images
Target data
Function
Binds to IL-18 and inhibits its activity. Functions as an inhibitor of the early TH1 cytokine response (By similarity).
What's included?
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Mouse IL-18BP ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse IL-18BP in Citrate plasma, Serum samples.
Alternative names=Interleukin-18-binding protein, IL-18BP, Interferon gamma-inducing factor-binding protein, Igifbp, Il18bp
Key facts
- Detection method
Colorimetric
- Sample types
Citrate plasma, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Mouse
- Range
468.75 - 30000 pg/mL
- Assay time
1h 30m
- Assay Platform
Pre-coated microplate (12 x 8 well strips)
- Sensitivity
= 151.82 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 3.7 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 97 | Range |
Sample type Citrate plasma | Average % = 98 | Range |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Mouse IL-18BP ELISA Kit (ab254509) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-18BP protein in cit plasma and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IL-18BP with 151.82 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
IL-18BP is a protein that binds to IL-18, preventing it from interacting with its receptor. IL-18BP inhibits the early TH1 cytokine response. IL-18 is produced constitutively by mononuclear cells.
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Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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