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AB326402

Mouse IL-19 ELISA Kit, Chemiluminescent

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Mouse IL-19 ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Mouse IL-19 with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 2.29 - 5000 pg/ml with a sensitivity of 0.68pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
-Patent pending

View Alternative Names

Interleukin-19, IL-19, Il19

4 Images
Sandwich ELISA - Mouse IL-19 ELISA Kit, Chemiluminescent (AB326402)
  • sELISA

Lab

Sandwich ELISA - Mouse IL-19 ELISA Kit, Chemiluminescent (AB326402)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Mouse IL-19 ELISA Kit, Chemiluminescent (AB326402)
  • sELISA

Lab

Sandwich ELISA - Mouse IL-19 ELISA Kit, Chemiluminescent (AB326402)

Dynamic range comparison between the Chemiluminescent Mouse IL-19 SimpleStep ELISA, colorimetric Mouse IL-19 SimpleStep ELISA (ab253220), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and dynamic range.

Sandwich ELISA - Mouse IL-19 ELISA Kit, Chemiluminescent (AB326402)
  • sELISA

Lab

Sandwich ELISA - Mouse IL-19 ELISA Kit, Chemiluminescent (AB326402)

Standard curve comparison between the Chemiluminescent Mouse IL-19 SimpleStep ELISA and colorimetric Mouse IL-19 SimpleStep ELISA (ab253220) in Sample Diluent NS + 1X Enhancer. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse IL-19 ELISA Kit, Chemiluminescent (AB326402)
  • sELISA

Unknown

Sandwich ELISA - Mouse IL-19 ELISA Kit, Chemiluminescent (AB326402)

Interpolated concentrations of spike IL-19 in mouse plasma and cell culture media samples.

The concentrations of IL-19 were measured in duplicates interpolated from the IL-19 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 12.5% plasma (citrate) 25% plasma (EDTA) 25% plasma (heparin) 25% and cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2).

Key facts

Detection method

Luminescent

Sample types

Serum, EDTA Plasma, Cell culture media, Heparin Plasma, Citrate plasma

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.68 pg/mL

Range

2.29 - 5000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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Product details

Mouse IL-19 ELISA Kit, Chemiluminescent (ab326402) is a rapid single-wash sandwich 55-min ELISA to measure Mouse IL-19 in Serum, EDTA Plasma, Cell culture media, Heparin Plasma, Citrate plasma with a sensitivity of 0.68 pg/mL.

How the assay works

Mouse IL-19 ELISA Kit, Chemiluminescent (ab326402) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse IL-19 (ab326402) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Mouse IL19 antibody [EPR21878-258] - BSA and Aside free (Capture) ab259693
- Anti-Mouse IL19 antibody [EPR21878-255] - BSA and Aside free (Detector) ab259694
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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.1" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2" } ]
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Recovery

[ { "sample": "Serum", "range": "111 - 127 %", "average": "= 119" }, { "sample": "EDTA Plasma", "range": "91 - 109 %", "average": "= 96" }, { "sample": "Cell culture media", "range": "103 - 118 %", "average": "= 110" }, { "sample": "Heparin Plasma", "range": "103 - 118 %", "average": "= 111" }, { "sample": "Citrate plasma", "range": "75 - 88 %", "average": "= 81" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab326402-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB326402-CMP11", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB326402-CMP04", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB326402-CMP06", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent B", "number":"AB326402-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse IL-19 Capture Antibody", "number":"AB326402-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse IL-19 Lyophilized Recombinant Protein", "number":"AB326402-CMP03", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent A", "number":"AB326402-CMP08", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB326402-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB326402-CMP10", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB326402-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse IL-19 Detector Antibody", "number":"AB326402-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Cytokine that functions as an anti-inflammatory and proangiogenic factor (PubMed : 12370360, PubMed : 27053520). Polarizes adaptive immunity to an anti-inflammatory phenotype through induction of T-helper 2 responses by both down-regulation of IFN-gamma and up-regulation of IL4 and IL5 (PubMed : 15557163). Produced by osteocytes, stimulates granulopoiesis and neutrophil formation (PubMed : 33684929). Exerts its biological effect through a receptor complex consisting of a heterodimer of IL20RA and IL20RB. In turn, activates the Janus kinase (JAK) and signal transducer and activator of transcription (STAT) pathway, and importantly, STAT3 (By similarity).
See full target information Il19
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websiteProtocolBooklet
en
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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