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AB113348

Mouse IL-1ra ELISA Kit

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(2 Publications)

Mouse IL-1ra ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse IL-1ra in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Il-1ra, Il1rn, Interleukin-1 receptor antagonist protein, IL-1RN, IL-1ra, IRAP, IL1 inhibitor

6 Images
Sandwich ELISA - Mouse IL-1ra ELISA Kit (AB113348)
  • sELISA

Unknown

Sandwich ELISA - Mouse IL-1ra ELISA Kit (AB113348)

msIL-1Ra measured in biological fluids, background signal subtracted (duplicates +/- SD).

Sandwich ELISA - Mouse IL-1ra ELISA Kit (AB113348)
  • sELISA

Unknown

Sandwich ELISA - Mouse IL-1ra ELISA Kit (AB113348)

Standard curve of msIL-1Ra in different diluents with background signal subtracted (duplicates; +/- SD).

Sandwich ELISA - Mouse IL-1ra ELISA Kit (AB113348)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-1ra ELISA Kit (AB113348)

Representative Standard Curve using ab113348

Sandwich ELISA - Mouse IL-1ra ELISA Kit (AB113348)
  • sELISA

Unknown

Sandwich ELISA - Mouse IL-1ra ELISA Kit (AB113348)

IL-1Ra measured in mouse tissue lysates (expressed as per mg of extracted protein), with background signal subtracted (duplicates; +/- SD).

Sandwich ELISA - Mouse IL-1ra ELISA Kit (AB113348)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-1ra ELISA Kit (AB113348)

Representative Standard Curve using ab113348

Sandwich ELISA - Mouse IL-1ra ELISA Kit (AB113348)
  • sELISA

Unknown

Sandwich ELISA - Mouse IL-1ra ELISA Kit (AB113348)

IL-1Ra detected in supernatants from RAW 246.7 control cells (C) or cells stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297) (P), or 24 hours with PMA and 1 ug x mL-1 of LPS (Sigma) (P+L) for the last 6 hours. Results shown after background signal was subtracted (duplicates +/- SD).

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

< 2 pg/mL

Range

2.74 - 2000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's IL-1ra Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of IL-1ra in serum, plasma (collect plasma using EDTA and heparin as an anticoagulant. Citrate is not recommended for this assay) and cell culture supernates.

This assay employs an antibody specific for mouse IL-1ra coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1ra present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-1ra antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1ra bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "94 - 103 %", "average": "= 102" }, { "sample": "Serum", "range": "67 - 84 %", "average": "= 75.89" }, { "sample": "Plasma", "range": "71 - 111 %", "average": "= 93.69" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab113348-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB113348-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB113348-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB113348-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D", "number":"AB113348-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin Concentrate", "number":"AB113348-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Mouse IL-1ra", "number":"AB113348-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IL-1ra Microplate (12 x 8 wells)", "number":"AB113348-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse IL-1ra Standard (lyophilized)", "number":"AB113348-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB113348-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Anti-inflammatory antagonist of interleukin-1 family of proinflammatory cytokines such as interleukin-1beta/IL1B and interleukin-1alpha/IL1A. Protects from immune dysregulation and uncontrolled systemic inflammation triggered by IL1 for a range of innate stimulatory agents such as pathogens.
See full target information Il1rn
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

iScience 26:106953 PubMed37332598

2023

SENP3-mediated deSUMOylation of c-Jun facilitates microglia-induced neuroinflammation after cerebral ischemia and reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Xia,Meng Mao,Gaofeng Zhan,Zhenzhao Luo,Yin Zhao,Xing Li

JCI insight 3: PubMed29618659

2018

Mesenchymal TNFR2 promotes the development of polyarthritis and comorbid heart valve stenosis.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Sakkou,Panagiotis Chouvardas,Lydia Ntari,Alejandro Prados,Kristin Moreth,Helmut Fuchs,Valerie Gailus-Durner,Martin Hrabe de Angelis,Maria C Denis,Niki Karagianni,George Kollias
View all publications
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websiteProtocolBooklet
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