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AB238258

Mouse IL-1RA ELISA Kit

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Mouse IL-1RA ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse IL-1RA with a sensitivity of 10.3 pg/well. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Il-1ra, Il1rn, Interleukin-1 receptor antagonist protein, IL-1RN, IL-1ra, IRAP, IL1 inhibitor

4 Images
ELISA - Mouse IL-1RA ELISA Kit (AB238258)
  • ELISA

Supplier Data

ELISA - Mouse IL-1RA ELISA Kit (AB238258)
Sandwich ELISA - Mouse IL-1RA ELISA Kit (AB238258)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-1RA ELISA Kit (AB238258)

Example of mouse IL1RA standard curve in Sample Diluent NS. The IL1RA standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse IL-1RA ELISA Kit (AB238258)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-1RA ELISA Kit (AB238258)

Interpolated concentrations of native IL1RA in mouse serum and plasma samples. The concentrations of IL1RA were measured in duplicates, interpolated from the IL1RA standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25% plasma (citrate) and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL1RA concentration was determined to be 200 pg/mL in serum, 216 pg/mL in plasma (citrate) and 488 pg/mL in plasma (heparin).

Sandwich ELISA - Mouse IL-1RA ELISA Kit (AB238258)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-1RA ELISA Kit (AB238258)

Interpolated concentrations of native IL1RA in mouse cell culture supernatant samples.

The concentrations of IL1RA were measured in duplicates, interpolated from the IL1RA standard curves and corrected for sample dilution. Undiluted samples are as follows : J774A.1 unstimulated 100%, and J774A.1 stimulated 12.5% The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL1RA concentration was determined to be 426 pg/mL in unstimulated J774A.1 and 8,581 pg/mL in stimulated J774A.1 cell culture supernatant.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 10.3 pg/well

Range

46.88 - 3000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse IL-1RA ELISA Kit ab238258 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse IL-1RA in citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 10.3 pg/weLL.

How the assay works

Mouse IL-1RA SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse IL-1RA ELISA Kit ab238258 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-IL-1RA antibody [EPR17553-193] - BSA and Azide free (Capture) ab244793
- Anti-IL-1RA antibody [EPR17553-198] - BSA and Azide free (Detector) ab245066
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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.1" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.6" } ]
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Recovery

[ { "sample": "Serum", "range": "99 - 108 %", "average": "= 103" }, { "sample": "EDTA Plasma", "range": "93 - 98 %", "average": "= 96" }, { "sample": "Heparin Plasma", "range": "112 - 126 %", "average": "= 121" }, { "sample": "Citrate plasma", "range": "95 - 106 %", "average": "= 100" }, { "sample": "Cell culture media", "range": "114 - 120 %", "average": "= 116" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab238258-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB238258-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB238258-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB238258-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB238258-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB238258-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB238258-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB238258-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse IL-1RA Capture Antibody", "number":"AB238258-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse IL-1RA Lyophilized recombinant Protein", "number":"AB238258-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse IL-1RA Detector Antibody", "number":"AB238258-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Anti-inflammatory antagonist of interleukin-1 family of proinflammatory cytokines such as interleukin-1beta/IL1B and interleukin-1alpha/IL1A. Protects from immune dysregulation and uncontrolled systemic inflammation triggered by IL1 for a range of innate stimulatory agents such as pathogens.
See full target information Il1rn
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websiteProtocolBooklet
en
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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