Mouse IL-1RA ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse IL-1RA in Heparin Plasma, Citrate plasma, Serum, EDTA Plasma samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 46.88 - 3000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 10.3 pg/well
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Anti-inflammatory antagonist of interleukin-1 family of proinflammatory cytokines such as interleukin-1beta/IL1B and interleukin-1alpha/IL1A. Protects from immune dysregulation and uncontrolled systemic inflammation triggered by IL1 for a range of innate stimulatory agents such as pathogens.
Alternative names
Il-1ra, Il1rn, Interleukin-1 receptor antagonist protein, IL-1RN, IL-1ra, IRAP, IL1 inhibitor
What's included?
Recommended products
Mouse IL-1RA ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse IL-1RA in Heparin Plasma, Citrate plasma, Serum, EDTA Plasma samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 46.88 - 3000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 10.3 pg/well
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Supernatant | n 5 | mean - | SD - | C.V. 2.6 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Supernatant | n 3 | mean - | SD - | C.V. 3.1 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 103 | Range 99 - 108 % |
Sample type EDTA Plasma | Average % = 96 | Range 93 - 98 % |
Sample type Heparin Plasma | Average % = 121 | Range 112 - 126 % |
Sample type Citrate plasma | Average % = 100 | Range 95 - 106 % |
Sample type Cell culture media | Average % = 116 | Range 114 - 120 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse IL-1RA ELISA Kit (ab238258) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-1RA protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IL-1RA with 10.3 pg/well sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Product promise
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3 product images
Sandwich ELISA - Mouse IL-1RA ELISA Kit (ab238258)
Example of mouse IL1RA standard curve in Sample Diluent NS. The IL1RA standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse IL-1RA ELISA Kit (ab238258)
Interpolated concentrations of native IL1RA in mouse cell culture supernatant samples.
The concentrations of IL1RA were measured in duplicates, interpolated from the IL1RA standard curves and corrected for sample dilution. Undiluted samples are as follows: J774A.1 unstimulated 100%, and J774A.1 stimulated 12.5% The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL1RA concentration was determined to be 426 pg/mL in unstimulated J774A.1 and 8,581 pg/mL in stimulated J774A.1 cell culture supernatant.
Sandwich ELISA - Mouse IL-1RA ELISA Kit (ab238258)
Interpolated concentrations of native IL1RA in mouse serum and plasma samples. The concentrations of IL1RA were measured in duplicates, interpolated from the IL1RA standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25% plasma (citrate) and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL1RA concentration was determined to be 200 pg/mL in serum, 216 pg/mL in plasma (citrate) and 488 pg/mL in plasma (heparin).
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