AB325254

Mouse IL-2 ELISA Kit

SimpleStep
What is this?

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Mouse IL-2 ELISA Kit is a Sandwich ELISA for the measurement of Mouse IL-2 in Mouse in Other samples, Biofluids samples.

View Alternative Names

Il-2, Il2, Interleukin-2, IL-2, T-cell growth factor, TCGF

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Sandwich ELISA - Mouse IL-2 ELISA Kit (AB325254)

sELISA

Lab

Sandwich ELISA - Mouse IL-2 ELISA Kit (AB325254)

Interpolated concentrations of native mouse IL-2 was measured in duplicate at different sample concentrations. Undiluted samples are 8% unstimulated and stimulated EL4.IL-2 cell culture supernatant. EL4.IL-2 cells were cultured in RPMI supplemented with 10% fetal bovine serum and stimulated with 10 µg/mL PHA and 10 ng/mL PMA for 48 hrs. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Lab

Sandwich ELISA - Mouse IL-2 ELISA Kit (AB325254)

Interpolated concentration of native IL-2 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 8% EL4.IL-2 cell culture supernatant (stimulated with 10 µg/mL PHA and 10 ng/mL PMA for 48 hrs). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Lab

Sandwich ELISA - Mouse IL-2 ELISA Kit (AB325254)

Example of mouse IL-2 standard curve in 96-well vs. 384-well plate in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Mouse IL-2 ELISA Kit (AB325254)

Standard curve comparison between Mouse IL-2 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. The SimpleStep ELISA kit shows comparable sensitivity.

sELISA

Lab

Sandwich ELISA - Mouse IL-2 ELISA Kit (AB325254)

Example of mouse IL-2 standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Supernatant, Serum, Citrate plasma, EDTA Plasma, Heparin Plasma

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 5.31 pg/mL

Range

15 - 960 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse IL-2 ELISA Kit (ab325254) is a rapid single-wash sandwich 90-min ELISA to measure Mouse IL-2 in Serum, Plasma – Citrate, Plasma – EDTA, Plasma – Heparin, Supernatant. This SimpleStep sensitivity is 5.31 pg/ml.

How the assay works

Mouse IL-2 ELISA Kit (ab325254) SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse IL-2 (ab325254) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Save your precious samples

Our Mouse IL-2 ELISA Kit only requires 12.5µL sample.

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

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Precision

[ { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 9.8" }, { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 12.4" } ]

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Recovery

[ { "sample": "Serum", "range": "97 - 115 %", "average": "= 106" }, { "sample": "Citrate plasma", "range": "91 - 105 %", "average": "= 97" }, { "sample": "EDTA Plasma", "range": "96 - 112 %", "average": "= 103" }, { "sample": "Heparin Plasma", "range": "89 - 113 %", "average": "= 102" }, { "sample": "Cell culture supernatant", "range": "102 - 115 %", "average": "= 109" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Cytokine produced by activated CD4-positive helper T-cells and to a lesser extend activated CD8-positive T-cells and natural killer (NK) cells that plays pivotal roles in the immune response and tolerance (PubMed : 14614860, PubMed : 9814585). Binds to a receptor complex composed of either the high-affinity trimeric IL-2R (IL2RA/CD25, IL2RB/CD122 and IL2RG/CD132) or the low-affinity dimeric IL-2R (IL2RB and IL2RG). Interaction with the receptor leads to oligomerization and conformation changes in the IL-2R subunits resulting in downstream signaling starting with phosphorylation of JAK1 and JAK3. In turn, JAK1 and JAK3 phosphorylate the receptor to form a docking site leading to the phosphorylation of several substrates including STAT5 (PubMed : 14614860, PubMed : 27018889). This process leads to activation of several pathways including STAT, phosphoinositide-3-kinase/PI3K and mitogen-activated protein kinase/MAPK pathways. Functions as a T-cell growth factor and can increase NK-cell cytolytic activity as well. Promotes strong proliferation of activated B-cells and subsequently immunoglobulin production. Plays a pivotal role in regulating the adaptive immune system by controlling the survival and proliferation of regulatory T-cells, which are required for the maintenance of immune tolerance (PubMed : 14614860). Moreover, participates in the differentiation and homeostasis of effector T-cell subsets, including Th1, Th2, Th17 as well as memory CD8-positive T-cells (PubMed : 9814585).

See full target information Il2

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websiteProtocolBooklet

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Product promise

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