JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB46096

Mouse IL-2 ELISA Kit

Be the first to review this product! Submit a review

|

(3 Publications)

Mouse IL-2 ELISA Kit is a Sandwich ELISA for the measurement of Mouse IL-2 in Mouse in Cell Culture Media, Biofluids samples.

View Alternative Names

Il-2, Il2, Interleukin-2, IL-2, T-cell growth factor, TCGF

1 Images
Sandwich ELISA - Mouse IL-2 ELISA Kit (AB46096)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-2 ELISA Kit (AB46096)

Representative Standard Curve using ab46096.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 3.4 pg/mL

Range

15.6 - 500 pg/mL

Assay time

3h 45m

Assay Platform

Microplate

style
left-column

Complementary Products

Assay Kits

AB208348

Mouse TNF alpha ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Mouse TNF alpha ELISA Kit (AB208348)

  • 4
  • 4
Assay Kits

AB282874

Mouse IFN gamma ELISA Kit

Recombinant|SimpleStep

ELISA - Mouse IFN gamma ELISA Kit (AB282874)

  • 0
  • 0
Assay Kits

AB222503

Mouse IL-6 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Mouse IL-6 ELISA Kit (AB222503)

  • 5
  • 2
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Abcam's IL-2 mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-2 in mouse serum, buffered solutions and cell culture medium.

A monoclonal antibody specific for IL-2 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-2 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-2 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-2 present in the samples.

This kit will recognize both endogenous and recombinant mouse IL-2.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Sample B", "replicates": 8, "mean": "137 pg/mL", "standardDeviation": "8.9", "coefficientOfVariability": "6.4" }, { "reproducibilityType": "Intra", "sample": "Sample A", "replicates": 10, "mean": "170 pg/mL", "standardDeviation": "13", "coefficientOfVariability": "7.8" } ]
style
left-column

What's included?

{ "values": { "2x96Tests": { "sellingSize": "2 x 96 Tests", "publicAssetCode":"ab46096-2x96Tests", "assetComponentDetails": [ { "size":"1 x 23 mL", "name":"HRP Diluent", "number":"AB46096-CMP03" }, { "size":"2 x 10 mL", "name":"200X Wash Buffer", "number":"AB46096-CMP05" }, { "size":"1 x 25 mL", "name":"10X Standard Diluent Buffer", "number":"AB46096-CMP07" }, { "size":"1 x 24 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46096-CMP09" }, { "size":"4 x 1 Vial", "name":"Murine IL-2 standard (Lyophilized)", "number":"AB46096-CMP06" }, { "size":"4 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46096-CMP04" }, { "size":"1 x 13 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46096-CMP01" }, { "size":"2 x 11 mL", "name":"Stop Reagent", "number":"AB46096-CMP02" }, { "size":"2 x 400 µL", "name":"IL-2 Biotinylated Detection antibody", "number":"AB46096-CMP08" }, { "size":"2 x 1 Unit", "name":"IL-2 Microplate (12 x 8 well strips)", "number":"AB46096-CMP10" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab46096-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"HRP Diluent", "number":"AB46096-CMP03", "productcode":"" }, { "size":"1 x 11 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46096-CMP09", "productcode":"" }, { "size":"1 x 7.5 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46096-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Murine IL-2 standard (Lyophilized)", "number":"AB46096-CMP06", "productcode":"" }, { "size":"2 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46096-CMP04", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Reagent", "number":"AB46096-CMP02", "productcode":"" }, { "size":"1 x 400 µL", "name":"IL-2 Biotinylated Detection antibody", "number":"AB46096-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"10X Standard Diluent Buffer", "number":"AB46096-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IL-2 Microplate (12 x 8 well strips)", "number":"AB46096-CMP10", "productcode":"" }, { "size":"1 x 10 mL", "name":"200X Wash Buffer", "number":"AB46096-CMP05", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column
style
left-column

Product protocols

style
left-column

Target data

Cytokine produced by activated CD4-positive helper T-cells and to a lesser extend activated CD8-positive T-cells and natural killer (NK) cells that plays pivotal roles in the immune response and tolerance (PubMed : 14614860, PubMed : 9814585). Binds to a receptor complex composed of either the high-affinity trimeric IL-2R (IL2RA/CD25, IL2RB/CD122 and IL2RG/CD132) or the low-affinity dimeric IL-2R (IL2RB and IL2RG). Interaction with the receptor leads to oligomerization and conformation changes in the IL-2R subunits resulting in downstream signaling starting with phosphorylation of JAK1 and JAK3. In turn, JAK1 and JAK3 phosphorylate the receptor to form a docking site leading to the phosphorylation of several substrates including STAT5 (PubMed : 14614860, PubMed : 27018889). This process leads to activation of several pathways including STAT, phosphoinositide-3-kinase/PI3K and mitogen-activated protein kinase/MAPK pathways. Functions as a T-cell growth factor and can increase NK-cell cytolytic activity as well. Promotes strong proliferation of activated B-cells and subsequently immunoglobulin production. Plays a pivotal role in regulating the adaptive immune system by controlling the survival and proliferation of regulatory T-cells, which are required for the maintenance of immune tolerance (PubMed : 14614860). Moreover, participates in the differentiation and homeostasis of effector T-cell subsets, including Th1, Th2, Th17 as well as memory CD8-positive T-cells (PubMed : 9814585).
See full target information Il2
style
left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Saudi journal of biological sciences 28:4751-4761 PubMed34354463

2021

evidence: Repression of mucosal immune responses in mice with colon cancer following sustained administration of .

Applications

Unspecified application

Species

Unspecified reactive species

Sahar El Hadad,Maha Alsolami,Alia Aldahlawi,Jehan Alrahimi,Fatemah Basingab,Shahira Hassoubah,Hani Alothaid

Oxidative medicine and cellular longevity 2018:2474831 PubMed29636837

2018

Nephroprotective Effect of Zingerone against CCl-Induced Renal Toxicity in Swiss Albino Mice: Molecular Mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammed M Safhi

ACS nano 11:11433-11440 PubMed29124929

2017

Injectable Polymeric Cytokine-Binding Nanowires Are Effective Tissue-Specific Immunomodulators.

Applications

ELISA

Species

Mouse

Colin R Zamecnik,Margaret M Lowe,David M Patterson,Michael D Rosenblum,Tejal A Desai
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com