JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB267643

Mouse IL-2 R alpha ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Mouse IL-2 R alpha ELISA Kit is a Sandwich ELISA for the measurement of Mouse IL-2 R alpha in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

CD25, Il2r, Il2ra, Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, p55

1 Images
Sandwich ELISA - Mouse IL-2 R alpha ELISA Kit (AB267643)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-2 R alpha ELISA Kit (AB267643)

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 10 pg/mL

Range

10.24 - 2500 pg/mL

Assay Platform

Microplate

style
left-column

Complementary Products

Assay Kits

AB157713

Mouse Ferritin ELISA Kit (FTL)

Sandwich ELISA - Mouse Ferritin ELISA Kit (FTL) (AB157713)

  • 5
  • 2
Assay Kits

AB315788

Mouse Fibrinogen ELISA Kit- Extracellular

SimpleStep

Sandwich ELISA - Mouse Fibrinogen ELISA Kit- Extracellular (AB315788)

  • 0
  • 0
Assay Kits

AB260067

Mouse IP-10 ELISA Kit (CXCL10)

SimpleStep

ELISA - Mouse IP-10 ELISA Kit (CXCL10) (AB260067)

  • 0
  • 0
Assay Kits

AB255729

Mouse IL-10 ELISA Kit

Recombinant|SimpleStep

ELISA - Mouse IL-10 ELISA Kit (AB255729)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Mouse IL-2 R alpha ELISA Kit is designed for the quantitative determination of IL-2 R alpha in cell culture supernatants, plasma and serum samples.

This assay employs an antibody specific for mouse IL-2 R alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-2 R alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-2 R alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-2 R alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

This ELISA kit detects mouse IL-2R alpha, less than 0.1% cross-reactivity with human IL-2R alpha is observed.

style
left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
style
left-column

Recovery

[ { "sample": "Serum", "range": "85 - 105 %", "average": "= 96.14" }, { "sample": "Plasma", "range": "68 - 107 %", "average": "= 82.55" }, { "sample": "Cell culture media", "range": "92 - 124 %", "average": "= 105.7" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab267643-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB267643-CMP01", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB267643-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse IL-2 R alpha Standard (Lyophilized)", "number":"AB267643-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB267643-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Mouse IL-2 R alpha coated Microplate (12 x 8 wells)", "number":"AB267643-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"250X HRP-Streptavidin Concentrate", "number":"AB267643-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB267643-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Mouse IL-2 R alpha Detection Antibody", "number":"AB267643-CMP08", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column
style
left-column

Product protocols

style
left-column

Target data

Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.
See full target information Il2ra
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com