Mouse IL-2 R alpha ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Mouse IL-2 R alpha in Mouse in Plasma, Cell culture supernatant, Serum samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 10.24 - 2500 pg/mL
- Sensitivity
- = 10 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.
Alternative names
CD25, Il2r, Il2ra, Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, p55
What's included?
Recommended products
Mouse IL-2 R alpha ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Mouse IL-2 R alpha in Mouse in Plasma, Cell culture supernatant, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 10.24 - 2500 pg/mL
- Assay Platform
- Microplate
- Sensitivity
- = 10 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 10 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 12 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 96.14 | Range 85 - 105 % |
Sample type Plasma | Average % = 82.55 | Range 68 - 107 % |
Sample type Cell culture media | Average % = 105.7 | Range 92 - 124 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Mouse IL-2 R alpha ELISA Kit is designed for the quantitative determination of IL-2 R alpha in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for mouse IL-2 R alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-2 R alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-2 R alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-2 R alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This ELISA kit detects mouse IL-2R alpha, less than 0.1% cross-reactivity with human IL-2R alpha is observed.
Product promise
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1 product image
Sandwich ELISA - Mouse IL-2 R alpha ELISA Kit (ab267643)
This standard curve is for demonstration only. A standard curve must be run with each assay.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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