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AB100707

Mouse IL-21 ELISA Kit

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Mouse IL-21 ELISA Kit is a Sandwich ELISA for the measurement of Mouse IL-21 in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Interleukin-21, IL-21, Il21

2 Images
Sandwich ELISA - Mouse IL-21 ELISA Kit (AB100707)
  • sELISA

Unknown

Sandwich ELISA - Mouse IL-21 ELISA Kit (AB100707)

Representative standard curve using ab100707

Sandwich ELISA - Mouse IL-21 ELISA Kit (AB100707)
  • sELISA

Unknown

Sandwich ELISA - Mouse IL-21 ELISA Kit (AB100707)

Representative standard curve using ab100707

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 6 pg/mL

Range

8.19 - 2000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's IL-21 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-21 in plasma and cell culture supernatants. (Mouse IL-21 concentration is pretty low in normal plasma, it may not be detected in this assay).

This assay employs an antibody specific for mouse IL-21 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-21 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-21 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-21 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

We have not been able to detect endogenous Mouse IL-21 in normal serum with ab100707, only in serum spiked with Mouse IL-21.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "87 - 109 %", "average": "= 98.65" }, { "sample": "Plasma", "range": "68 - 87 %", "average": "= 77.38" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100707-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100707-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100707-CMP06", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100707-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IL-21 Microplate (12 x 8 wells)", "number":"AB100707-CMP01", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100707-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100707-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-mouse IL-21", "number":"AB100707-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant mouse IL-21 Standard (lyophilized)", "number":"AB100707-CMP02", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB100707-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells (PubMed : 15100251). Implicated in the generation and maintenance of T follicular helper (Tfh) cells and the formation of germinal-centers. Together with IL6, control the early generation of Tfh cells and are critical for an effective antibody response to acute viral infection (PubMed : 23045607). May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15 (PubMed : 11970879). May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells (By similarity). During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation (PubMed : 12893770).
See full target information Il21
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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