AB282870

Mouse IL-21 ELISA Kit

Recombinant
SimpleStep
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(1 Publication)

Mouse IL-21 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse IL-21 with a sensitivity of 10.45 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Interleukin-21, IL-21, Il21

2 Images

Sandwich ELISA - Mouse IL-21 ELISA Kit (AB282870)

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-21 ELISA Kit (AB282870)

Interpolated concentrations of spiked IL-21 in mouse serum and plasma EDTA.

The concentrations of IL-21 were measured in duplicates, interpolated from the IL-21 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50% and plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-21 ELISA Kit (AB282870)

Example of mouse IL-21 standard curve in Sample Diluent 75BP.

The IL-21 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 10.45 pg/mL

Range

62.5 - 4000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse IL-21 ELISA kit (ab272199) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse IL-21 protein in mouse serum and plasma samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IL-21 with 10.45 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Mouse IL-21 is a cytokine involved in the regulation between the innate and adaptive immunity response. It is primarily expressed by activated T-cells. It has been suggested to have anti-tumor activity and may be involved in the development of autoimmunity.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.8" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.2" } ]

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Recovery

[ { "sample": "Serum", "range": "95 - 105 %", "average": "= 100" }, { "sample": "EDTA Plasma", "range": "113 - 120 %", "average": "= 116" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab282870-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB282870-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB282870-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB282870-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB282870-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB282870-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB282870-CMP10", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent 75BP", "number":"AB282870-CMP05", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB282870-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse IL-21 Lyophilized Recombinant Protein", "number":"AB282870-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse IL-21 Capture Antibody", "number":"AB282870-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse IL-21 Detector Antibody", "number":"AB282870-CMP04", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells (PubMed : 15100251). Implicated in the generation and maintenance of T follicular helper (Tfh) cells and the formation of germinal-centers. Together with IL6, control the early generation of Tfh cells and are critical for an effective antibody response to acute viral infection (PubMed : 23045607). May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15 (PubMed : 11970879). May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells (By similarity). During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation (PubMed : 12893770).

See full target information Il21

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell communication and signaling : CCS 20:83 PubMed35658901

2022

Lactobacillus improves the effects of prednisone on autoimmune hepatitis via gut microbiota-mediated follicular helper T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Ma,Liwen Zhang,Yun Zhuang,Yanbo Ding,Jianping Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse IL-21 ELISA Kit

Sandwich ELISA - Mouse IL-21 ELISA Kit (AB282870)

Sandwich ELISA - Mouse IL-21 ELISA Kit (AB282870)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Assay Kits

Assay Kits

Secondary Antibodies

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (1)

Product promise