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AB223857

Mouse IL-22 ELISA Kit

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(2 Publications)

Mouse IL-22 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse IL-22 with a sensitivity of 0.15 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Il22a, Iltif, Iltifa, Il22, Interleukin-22, IL-22, IL-10-related T-cell-derived-inducible factor, IL-TIF alpha, Interleukin-22a, IL-TIF, IL-22a

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ELISA - Mouse IL-22 ELISA Kit (AB223857)
  • ELISA

Supplier Data

ELISA - Mouse IL-22 ELISA Kit (AB223857)
Sandwich ELISA - Mouse IL-22 ELISA Kit (AB223857)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-22 ELISA Kit (AB223857)

Interpolated concentrations of native IL-22 in stimulated EL4.IL2 and spleen cell culture supernatant.

The concentrations of IL-22 were measured in duplicate and interpolated from the IL-22 standard curve and corrected for sample dilution. Undiluted samples are as follows : EL4.IL2 supernatant 25% and spleen supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-22 concentration was determined to be 500 pg/mL in EL4.IL2 supernatant and 225 pg/mL in spleen supernatant.

Sandwich ELISA - Mouse IL-22 ELISA Kit (AB223857)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-22 ELISA Kit (AB223857)

Interpolated concentrations of native IL-22 in Mouse serum and plasma samples.

The concentrations of IL-22 were measured in duplicates, interpolated from the IL-22 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (citrate) 50%, plasma (heparin) 50% and plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-22 concentration was determined to be 117 pg/mL in serum, 101 pg/mL in plasma (citrate), 193 pg/mL in plasma (heparin), and 137 pg/mL in plasma (EDTA).

Sandwich ELISA - Mouse IL-22 ELISA Kit (AB223857)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-22 ELISA Kit (AB223857)

Example of Mouse IL-22 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse IL-22 ELISA Kit (AB223857)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-22 ELISA Kit (AB223857)

Comparison of IL-22 in unstimulated and stimulated cell culture supernatants.

EL4.IL2 cells were cultured to semi-confluency and then treated for 48 hours with either 10 μg/mL PHA + 10 ng/mL PMA or DMSO (mock). Rat spleen cells were cultured in the presence or absence of 100 ng/mL IFN-gamma + 1 μg/mL LPS for 4 days. The concentrations of IL-22 were measured in five different dilutions of the supernatant samples in duplicates and interpolated from the IL-22 standard curve. The interpolated values are plotted (mean +/- SD, n=5) in pg per mL of neat supernatant. The mean IL-22 concentration was determined to be 500 pg/mL in stimulated EL4.IL2 supernatant and undetectable in unstimulated EL4.IL2 supernatant. The mean concentration was determined to be 225 pg/mL in stimulated spleen supernatant and 1.7 pg/mL in unstimulated spleen supernatant.

Sandwich ELISA - Mouse IL-22 ELISA Kit (AB223857)
  • sELISA

Lab

Sandwich ELISA - Mouse IL-22 ELISA Kit (AB223857)

Mouse IL-22 standard curve comparison data.

Standard curve comparison between mouse IL-22 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 0.15 pg/mL

Range

3.13 - 200 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse IL-22 ELISA Kit ab223857 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse IL-22 in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 0.15 pg/mL.

How the assay works

Mouse IL-22 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse IL-22 ELISA Kit ab223857 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-IL-22 antibody [EPR20468-273] - BSA and Azide free (Capture) ab244928
- Anti-IL-22 antibody [EPR20468-222] - BSA and Azide free (Detector) ab244928

Interleukin 22 (IL-22) is a cytokine thought to be involved in the response to inflammation through the Jak/STAT and MAPK signaling pathways. IL-22 is produced by NK cells and Th1-type T cells and can stimulate the induction of pro-inflammatory cytokines in human keratinocytes.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.2" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 9, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.8" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "104 - 110 %", "average": "= 107" }, { "sample": "Serum", "range": "98 - 101 %", "average": "= 99" }, { "sample": "EDTA Plasma", "range": "98 - 101 %", "average": "= 100" }, { "sample": "Plasma", "range": "104 - 110 %", "average": "= 101" }, { "sample": "Citrate plasma", "range": "94 - 96 %", "average": "= 95" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab223857-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB223857-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB223857-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB223857-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB223857-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB223857-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse IL-22 Capture Antibody", "number":"AB223857-CMP02", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB223857-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse IL-22 Lyophilized Recombinant Protein", "number":"AB223857-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse IL-22 Detector Antibody", "number":"AB223857-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB223857-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Cytokine that plays a critical role in modulating tissue responses during inflammation (PubMed : 33852830, PubMed : 35525330). Plays an essential role in the regeneration of epithelial cells to maintain barrier function after injury and for the prevention of further tissue damage (PubMed : 33912578). Unlike most of the cytokines, has no effect on immune cells. Signals through a heterodimeric receptor composed of two subunits, the specific receptor IL22RA1 which is present on non-immune cells in many organs and the shared subunit IL10RB. Ligation of IL22RA1 with IL22 induces activation of the tyrosine kinases JAK1 and TYK2, which in turn activates STAT3. In turn, promotes cell survival and proliferation through STAT3, ERK1/2 and PI3K/AKT pathways. Promotes phosphorylation of GSK3B at 'Ser-9' and CTTN (PubMed : 24742671). Promotes epithelial cell spreading (PubMed : 24742671).
See full target information Il22
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Asian Pacific journal of cancer prevention : APJCP 22:3647-3661 PubMed34837924

2021

Chemopreventive Role of Black Tea Extract in Swiss Albino Mice Exposed to Inorganic Arsenic.

Applications

Unspecified application

Species

Unspecified reactive species

Archismaan Ghosh,Sutapa Mukherjee,Madhumita Roy

RSC advances 10:3371-3379 PubMed35497743

2020

Tryptophan promoted β-defensin-2 expression the mTOR pathway and its metabolites: kynurenine banding to aryl hydrocarbon receptor in rat intestine.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiru Tang,Baoshi Shi,Weizhong Sun,Yulong Yin,Qingju Chen,Taha Mohamed,Changwen Lu,Zhihong Sun
View all publications
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