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AB100708

Mouse IL-28 ELISA Kit

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(1 Publication)

Mouse IL-28 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse IL-28 in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Il28, Il28b, Ifnl3, Interferon lambda-3, IFN-lambda-3, Interleukin-28B, IL-28B

1 Images
Sandwich ELISA - Mouse IL-28 ELISA Kit (AB100708)
  • sELISA

Unknown

Sandwich ELISA - Mouse IL-28 ELISA Kit (AB100708)

Representative standard curve using ab100708

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

< 2 pg/mL

Range

2.46 - 600 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's IL-28 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IL-28 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for IL-28 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-28 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse IL-28 antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-28 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "67 - 87 %", "average": "= 74.3" }, { "sample": "Serum", "range": "98 - 118 %", "average": "= 109.8" }, { "sample": "Plasma", "range": "67 - 93 %", "average": "= 76.8" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100708-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100708-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100708-CMP07", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB100708-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IL-28 Microplate (12 x 8 wells)", "number":"AB100708-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100708-CMP05", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C", "number":"AB100708-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse IL-28 Standard (lyophilized)", "number":"AB100708-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Mouse IL-28", "number":"AB100708-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100708-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Cytokine with antiviral, antitumour and immunomodulatory activities. Plays a critical role in the antiviral host defense, predominantly in the epithelial tissues. Acts as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, and receptor engagement leads to the activation of the JAK/STAT signaling pathway resulting in the expression of IFN-stimulated genes (ISG), which mediate the antiviral state. Has a restricted receptor distribution and therefore restricted targets : is primarily active in epithelial cells and this cell type-selective action is because of the epithelial cell-specific expression of its receptor IFNLR1. Seems not to be essential for early virus-activated host defense in vaginal infection, but plays an important role in Toll-like receptor (TLR)-induced antiviral defense. Plays a significant role in the antiviral immune defense in the intestinal epithelium. Exerts an immunomodulatory effect by up-regulating MHC class I antigen expression.
See full target information Ifnl3

Additional targets

Ifnl2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of immunology (Baltimore, Md. : 1950) 192:2770-7 PubMed24532585

2014

IPS-1 is essential for type III IFN production by hepatocytes and dendritic cells in response to hepatitis C virus infection.

Applications

Unspecified application

Species

Unspecified reactive species

Masaaki Okamoto,Hiroyuki Oshiumi,Masahiro Azuma,Nobuyuki Kato,Misako Matsumoto,Tsukasa Seya
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websiteProtocolBooklet
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