Mouse IL-28A ELISA Kit
- Recombinant
- SimpleStep
- What is this?
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- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- sELISA
Supplier Data
Sandwich ELISA - Mouse IL-28A ELISA Kit (AB208989)
Interpolated concentrations of spiked IL-28A in mouse serum and plasma.
The concentrations of IL-28A were measured in duplicates, interpolated from the (target) standard curves and corrected for sample dilution. 1X diluted samples are as follows : serum 12.5% and plasma (citrate) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
- sELISA
Supplier Data
Sandwich ELISA - Mouse IL-28A ELISA Kit (AB208989)
Interpolated concentrations of spiked IL-28A in cell culture supernatant samples.
The concentrations of IL-28A were measured in duplicates, interpolated from the (target) standard curves and corrected for sample dilution. 1X diluted samples are as follows : lung cell culture supernatant 25%, and RAW 264.7 cell culture supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
- sELISA
Supplier Data
Sandwich ELISA - Mouse IL-28A ELISA Kit (AB208989)
Example of mouse IL-28A standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Complementary Products
Reactivity data
Product details
Mouse IL-28A ELISA Kit (ab208989) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-28A protein in cell culture supernatant, cit plasma, edta plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IL-28A with 2.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
IL-28A (Interleukin 28A), also known as IFN-lambda 2 (Interferon-lambda 2), is a class II cytokine receptor ligand with antiviral, antitumor, and immunomodulatory activities.
Precision & recovery information
Precision
[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.1" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.7" } ]
Recovery
[ { "sample": "Cell culture supernatant", "range": "102 - 107 %", "average": "= 105" }, { "sample": "Serum", "range": "110 - 112 %", "average": "= 111" }, { "sample": "EDTA Plasma", "range": "106 - 106 %", "average": "= 106" }, { "sample": "Citrate plasma", "range": "109 - 112 %", "average": "= 111" }, { "sample": "Cell culture media", "range": "106 - 108 %", "average": "= 108" } ]
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Product protocols
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Target data
Alternative Names
Product promise
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