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AB222509

Mouse IL-3 ELISA Kit

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Mouse IL-3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse IL-3 with a sensitivity of 12 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Csfmu, Il-3, Il3, Interleukin-3, IL-3, Hematopoietic growth factor, Mast cell growth factor, Multipotential colony-stimulating factor, P-cell-stimulating factor, MCGF

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ELISA - Mouse IL-3 ELISA Kit (AB222509)
  • ELISA

Supplier Data

ELISA - Mouse IL-3 ELISA Kit (AB222509)

Interpolated concentrations of spiked IL-3 in mouse serum, and plasma samples.

The concentrations of IL-3 were measured in duplicates, interpolated from the IL-3 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (heparin) 50% and plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

ELISA - Mouse IL-3 ELISA Kit (AB222509)
  • ELISA

Supplier Data

ELISA - Mouse IL-3 ELISA Kit (AB222509)

Example of mouse IL-3 standard curve.

The IL-3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

ELISA - Mouse IL-3 ELISA Kit (AB222509)
  • ELISA

Supplier Data

ELISA - Mouse IL-3 ELISA Kit (AB222509)

Interpolated concentrations of native IL-3 in an unstimulated and PHAM (10 µg/mL PHA and 10 ng/mL PMA, 48 hr) stimulated EL4 cell culture supernatant samples.

The concentrations of IL-3 were measured in duplicate and interpolated from the IL-3 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-3 concentration was determined to be 1834 pg/mL in neat stimulated EL4 culture supernatant samples and not detectable in unstimulated samples.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 12 pg/mL

Range

15.6 - 1000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse IL-3 ELISA Kit (ab222509) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-3 protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IL-3 with 12 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Interleukin 3 (IL-3) is a secreted 166 amino acids long, glycosylated interleukin coded by the IL3 gene. Previous names reported for IL-3 are Hematopoietic growth factor, Mast cell growth factor, multi-potential colony-stimulating factor, and P-cell-stimulating factor. IL-3 has been found to stimulate a variety of cell activities such as; proliferation, differentiation, survival of pluripotent hematopoietic stem cells, and may be associated with neurologic disorders. IL-3 is expressed by activated T-cells, mast cells, and natural killer cells. IL-3 performs its biological action by binding to a transmembrane receptor complex consisting of IL-3RA, also known as CD123, and a signal inducing beta subunit, which also acts as a receptor for colony stimulating factor 2 and Interleukin 5.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Standard", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.3" }, { "reproducibilityType": "Intra", "sample": "Standard", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.9" } ]
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Recovery

[ { "sample": "Serum", "range": "99 - 107 %", "average": "= 102" }, { "sample": "EDTA Plasma", "range": "90 - 107 %", "average": "= 99" }, { "sample": "Heparin Plasma", "range": "109 - 123 %", "average": "= 115" }, { "sample": "Citrate plasma", "range": "103 - 109 %", "average": "= 106" }, { "sample": "Cell culture media", "range": "96 - 113 %", "average": "= 103" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab222509-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB222509-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB222509-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB222509-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB222509-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB222509-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse IL-3 Capture Antibody", "number":"AB222509-CMP02", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB222509-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse IL-3 Lyophilized Recombinant Protein", "number":"AB222509-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse IL-3 Detector Antibody", "number":"AB222509-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB222509-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Cytokine secreted predominantly by activated T-lymphocytes as well as mast cells and osteoblastic cells that controls the production and differentiation of hematopoietic progenitor cells into lineage-restricted cells. Stimulates also mature basophils, eosinophils, and monocytes to become functionally activated. In addition, plays an important role in neural cell proliferation and survival. Participates as well in bone homeostasis and inhibits osteoclast differentiation by preventing NF-kappa-B nuclear translocation and activation. Mechanistically, exerts its biological effects through a receptor composed of IL3RA subunit and a signal transducing subunit IL3RB (By similarity). Receptor stimulation results in the rapid activation of JAK2 kinase activity leading to STAT5-mediated transcriptional program (PubMed : 10376805, PubMed : 31990690, PubMed : 8378315). Alternatively, contributes to cell survival under oxidative stress in non-hematopoietic systems by activating pathways mediated by PI3K/AKT and ERK (By similarity).
See full target information Il3
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websiteProtocolBooklet
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