AB243681

Mouse IL-31 ELISA Kit

Recombinant
SimpleStep
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(3 Publications)

Mouse IL-31 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse IL-31 with a sensitivity of 24 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Interleukin-31, IL-31, Il31

2 Images

Sandwich ELISA - Mouse IL-31 ELISA Kit (AB243681)

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-31 ELISA Kit (AB243681)

Example of mouse IL-31 standard curve in Sample Diluent 50BP.

The IL-31 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-31 ELISA Kit (AB243681)

Interpolated concentrations of spike IL-31 in mouse serum, plasma and cell culture supernatant samples.

The concentrations of IL-31 were measured in duplicates, interpolated from the IL-31 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 12.5%, plasma (EDTA) 12.5%, plasma (citrate) 12.5%, plasma (heparin) 12.5% and RPMI media 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 24 pg/mL

Range

78.1 - 5000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse IL-31 ELISA Kit (ab243681) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-31 protein in cit plasma, edta plasma, hep plasma, serum, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IL-31 with 24 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.1" } ]

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Recovery

[ { "sample": "Serum", "range": "84 - 97 %", "average": "= 90" }, { "sample": "EDTA Plasma", "range": "95 - 104 %", "average": "= 99" }, { "sample": "Cell culture media", "range": "83 - 94 %", "average": "= 90" }, { "sample": "Heparin Plasma", "range": "87 - 98 %", "average": "= 91" }, { "sample": "Citrate plasma", "range": "80 - 91 %", "average": "= 84" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab243681-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB243681-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB243681-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB243681-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB243681-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB243681-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB243681-CMP10", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent 50BP", "number":"AB243681-CMP03", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB243681-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse IL-31 Lyophilized Recombinant Protein", "number":"AB243681-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse IL-31 Capture Antibody", "number":"AB243681-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse IL-31 Detector Antibody", "number":"AB243681-CMP07", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR (PubMed : 15184896). May function in skin immunity (PubMed : 15184896). Enhances myeloid progenitor cell survival in vitro (PubMed : 17379091). Induces RETNLA and serum amyloid A protein expression in macrophages (PubMed : 25847241).

See full target information Il31

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 30: PubMed39129303

2024

leaf extract and its main component myricitrin inhibit itch‑related IL‑6 and IL‑31 by suppressing microglial inflammation and microglial‑mediated astrocyte activation.

Applications

Unspecified application

Species

Unspecified reactive species

Jae Young Shin,Byoung Ok Cho,Ji Hyeon Park,Eun Seo Kang,Jang Ho Kim,Hun Yong Ha,Young-Soo Kim,Seon Il Jang

Journal of cellular and molecular medicine 28:e18375 PubMed39039796

2024

Celastrol alleviates atopic dermatitis by regulating Ezrin-mediated mitochondrial fission and fusion.

Applications

Unspecified application

Species

Unspecified reactive species

Dandan Wang,Shan Jin,Hanye Liu,Xinyi Song,Hongyu Jin,Yilan Song,Hongwei Zhao,Liangchang Li,Guanghai Yan

Disease markers 2023:9049256 PubMed36712922

2023

Inhibition of Mast Cell Degranulation in Atopic Dermatitis by Celastrol through Suppressing MRGPRX2.

Applications

Unspecified application

Species

Unspecified reactive species

Ciyu Yao,Wenzhen Ye,Mengxue Chen

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse IL-31 ELISA Kit

Sandwich ELISA - Mouse IL-31 ELISA Kit (AB243681)

Sandwich ELISA - Mouse IL-31 ELISA Kit (AB243681)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Secondary Antibodies

Assay Kits

Assay Kits

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (3)

Product promise